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- 1 (2)
U}IIVERS iTY O? CAL]CUT
.i
-! l-<t *.- r.:'r
B.Sc i'"{icrobiology. (lvlai:r arrrl Sirbsiliary; r::cr-sirrr-ri:irr.ri.:lcgy
iir.:ain) courses - intro4uction of
internal assessment
impiemenred r,vir-r efir:ci i'ic.r, lc05 ac:niiiicn onwards
- approved - orclers
-
issued.
1
No'GAIiJl/8037/99
Read:
- r.
GEI\IERAL AI{I}.ICADINTIC
(2)
I
Daied. Calicut University p.o.2o-07-2005
ffi;; ;_;;-;;;;.
2. Minutes oimeeting "i;;;;;:;;;r;l;ili..*",rr,
o:'Facult;r cf Science heid on r g.05.2005.
2. Minu:es oirneeting of Acacemic cc,_rncii heic on i g-06-2005.
M.inutes oi.nreetins
OR.IJER
l.
The meetinq of Board of Studias in
considered the introducticn of intem
subsidian) coL:rses. irrdustria! ivlicrooi
Further resol,zed that Z0o/o out of rhe t
kept for internal assessment. The C,;ratj
2'
The meeting of Faculry of science heii ci:
t8.0i.2c05, vide item
minutes of Board of Studies without an.,
mcdirrcatr'on.
3'
No. l(14), approved
the
The meeting ol'A.cadernic councii h-'td
o;r 18.6.2c05, vide item No. II A (2g).approverj
the minutes oi Borirrl of stucies as s:.ricved
b' the Faculry of Science anci also the
rninutes of Facult,i of Science.
4'
Sanction is therelore accor'ce.l for impienrenting
the d.-cision
effect 2005 admission ohwards.
5.
of Board of Studies rvith
Orders are issued accordinsiv.
sd/_
REGTSTRA.R (G&A r)
For REGIST].R.AR
To
The Principals. of
coreges offoring B.sc N,,icrobioiog,v- cour.s,es
l.he
(Main and Subsidiarl)
and industriai
,-raicrouiciogry (,=.,ainr
Cop.v
to:-
Controller of F.xa,:r:na;i6q1 {-i6ivs:sitl
Iri,_-,i:rati,t:.i flei:tre,s/
DR, AR (ESc),/SFTFC
Order
OI"-FICEIT
L.VA:\J I 8037.do,:
)
r :
IIICROBIOLOG l.
Sclem e ct'- l:i:r Ir-i trlti r'1'
B.Sc
Ivlarks
E>-t./Int.
[{ours
B3
60120,
4
i vear
Main
Paper
Sub I
Biochemistry I
II
I General N'iicrobiolog.r
4
Bio Statistics ard Computer Aoplicaiiitr.'
-I
(Language 1.2, 3 are for 300 mar[<s) same
B.Com couise
8C
60120
as
13
II year
Main paper II N{icrobia! Taxonomy
Main paper III Medical Microbiology
80
60t20
Main paper IV Immunology
Main paper V Microbiologv Practical Exam
8C
60120
80
6020
100
80t20
\20
100120
4
Main paper VI Mircobioal Physiology
80
60t20
4
Main paper VII Envirorunental and Sanitation
Microbiology
80
60t20
4
8C
60,'20
4
80
60r'20
4
80
6020
4
180
1.1Al40
5
Total Marks (main)
10ir0
7601240
Sub I Biochemistn'
2(ta
II
(Examination
4
4
4
3
to be conducted over fwo
consecutive da;rs each 5 hrours duration)
Sub I Biochemistry
n Theory
Practical
Sub
III
II Biostatistics and Computer appiication
year
Main paper VIrl Food and
RgiiciritLrral
Microbiolo_s.r
Main paper IX Industrial Nlicrooioiogy
Main paper X lv{icrobial genetics and ger:etic
Engineering
Microbiolc,gv piacticals Fxarc iI (tc be
conducted on three consecuiive clays 5
hours each)
10,-l
Grand Tota.
i
+){.
160/40
\
B.Sc INDUSTRIAL MICR.OEI0LOGY (MAIN)
Scherne ofl Eraminaticns
I,lar,<s
Ext./Int.
Hours
8C
60120
4
80
60t20
4
genetic Engineering
80
60t20
+
Main paper 4 - Microbio process I
80
60120
4
100
80t20
3
Main paper 6 Mircobioprocess II
80
60t20
4
Main paper 7 Ivlicrobioprccess III
8C
60t'20
4
Main paper 8 N{icrc.bioprocess IV
80
60120
4
Main paper 9 lvlicrc.bioprocess V
80
60120
4
Main paper l0 Microbioprocess VI
80
60t20
4
180
t40/40
5
I ]'ear
Main Paper I Industry Oriented Fundan:ental
Microbiology
Sub I Biochemistry
Sub
I
II Chemistry (or) Maths (cr) Ccmputer
Application
(same as B.Cont Course)
II year
Main paper 2 - Microbial physiolo_ey
Main paper
Main paper
Practical I
3-
5-
Microbial genetics
and
Industrial Micrcbioiogy
(to be conducted over two consecutive days
ofeach 5 hours duration)
Sub I Biochemistry II (Theory
&
Practical)
Sub 2 Chemistry (or) Maths (cr) Comouter
Applications
III year
Main Paper l1 Industrial
Practical II
Microbiology
(Conducted over
3
days each
of 5 hours
Total Marks (rnain)
Sub 1 Biochemistry
Sub
2
Chemistry (or) Maths (or) Cor-nputer
Grand Total
U.rlfVLkSll,y OI C.+LfCLT
(
:-,os
tract
)
Implerrrerrt,:Lt-i-r cf th= schem€ dn(- syllabus
vf r.Sc iiicrobj-ology
(maj.n), irrc
Incustrial_ Niicrcr,ioloqy (main) anct _.si bioclrernistry
suo) fcr i'rj.crociclcgy un.r rncust.iif 'riicroOiology
(nraj.ns ) _ with
ef fect frcnr 1999 acnrii--ion sprrrdr(s orc,ers
issuec-.
(
==========a===
GEllZi{z,L ,- '_ AC-,:-J
i'Jo:G''rAr/9fi1/gg
'-.etec, calicr-it UnlversitT F.o., r7. ]n.2(t0(r
= = ========
= = == == = = == ===== = == = ==:=_
l-leA.:- 1)i:irrutes of rneetinr_
Of ioard Of StUc,j-s irr .ulicr,crlc,iology
helc cn 3.2.200o.
rneetins of racurry of science helc on zj.6.2ooo
?J::]"":"= "I
r..'t-tnutes
of meetinc. of
Council heli on B.?.ZOOO.
^s3{s5njc
OtiDE
R
The tsoarc of st rf i€s in r'licrotci.o-Iogy at its meeti.nc
helc on
3.2.2oo0, resolvec, ro revise
rhe exi^stiaE E.sc -r.1j.erc;i,;idi^i*Il"I
r: (ie) witir fec-rlat.isp of Gacrrr;;'
t II Secenc Language. The ooard =r
heme and syllabus accorcliog to
9 -2O^O admissi.on onwarcls.
is compulsory for both courses
biology')
for E.sc I'licrobiolbgy
AArr,-^
!i- - optlonal
- -l{u---1 subsir.iary
ccur6e ttre
^.-L-._:
eiostitistj.cs
is
anc con,futei-aiirica"Liorr- I'<-.rr rnc-usg51aI I'licr;bigrogv{,rrbi€ata
may oe one from Clrem,ietry, Iratherq5i
ications.
Bhe ,:car<., of Stucies also approved th
of above
mentionec: t.,icrobic;loEy ani lnCustrial
2 )-.r.'ir= Facurty of science at its rneeting
neri on 27.6. 2ooc,
approvec the nij_nutes of ooarc of .Studies.
3)T,re ACac€n:ic Council at its ;oee.rireg heli on Zg.7.ZOOO,
approvei. tire rninutes of uoari of stu,.
ies ;"-;;;r;r*r-tr
f,actrlty sf
Science anc: the minutes of Faculty ,rf-S"ience.
zl)Sancticn has therefore Deen ace:,rceci
fcr the j.riiplercentati:n
cf the sclteme.ancl :yrlaous of o.5c I\iicrobj,orogy
tij.crooioloEy (mair, ) anc oicchemistry i"""I ,iEn (main) anc rnoustriar
"ril"t from 1999_2ooo
5)Orcers are issuec^ acccriingl\,.
s<.
To
i.,EPij-.rY
/-
REGISi,ir/;I
( c6-A.
f,o.r REGIS. k-rlt
E'incipars of corleqes offering B.sc l"iicrcbjoloqy
lneanc
I nCustrial I"riercbiclogy
.
ccurse_s
.
C,rpy Eo:- CE/University Infs,rinaticn Centres,/
University Litsrra ry /SF fi
C
rQr,r ARTED/
l4
krc/ 7e/
n/
rY
lP--;j \
SECiIt (-,Fl
i.
OF.LER
I
ICLR
r)
/
":!-q t1t
-obiol.oqY
a t i' 4!g-ir =rr,3- 9-f :-I::::-in
- a.rli: iti-ugSZ1,Sgk-l-
Yeo!
maln PaP-r I
GencraL i''i-crcbi':'Ii
cjY
Suo PaPer I Li-: chem j-strY I
and
II ;i.-sr-ati-stics jcati-'-';r
I
-;P'I
",;rt-,rt.t
(Langua;-s 7, 2, 3 are fiE 3C)O irr'-rrk=')
'
sam€ es r'c ni c 'urse
ir
TT
tr:
aI
YvJ!
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-
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1n
i.*
,: ;-'=
II
I
., III
rr
f_r/
lricr' oial 'r ax- n ii'l/
il=clical i icr'- l--'i - I-c;!
irrrnun-,I-qY
(
4llcurs
.FJ
4 )'tr t tr.q
'
4 ri. urs
l).t Av;
8O
4
8C
iI . hc,ttr s
i'tuur s
4 h urs
8''
i.r-c,r.-or'I'c| t;i::nr.?'I
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exarn I
c.-niuctsc -var 2 c-ns.:ctrtive
at,.r=
cacn 5 :i-urs clurati' n )
---1 -
-.j.,- De
5
uD.
T
J-
Bi-chemilt,
II l'he;rY
F
II
iJir-
racti.ca I
statistics anc C;mPut=r
AFPIicati-n II
TT I veaf
-.=:;-r-
FhYsi' I'gY'
lla in Fa! - r \/I i:icr-biaI
11151e-6t--I anC
.'
VII invif.
jnitet
t n tii-cr- bi' l-EY
s
'\iIII I c ancl .1c,r ic'11ur;lI
, -icr ' oiL I' gY
IX Inc,ustr i..rI Licr u1 I
qY
' 'o"+.'L'i' 'ln'l
.
i'-:
-.. ,r.-t i C -n(. f,, - -f I riq
80
4
40
1
h ,urs
h urs
120
4 h- urs
80
!iluuf s
60
hi,
8O
4 hg
uf
80
4 tre
ur-'
EC
4 h' urs
urs
5
(c.nrc-'2)
)
-2
Ieq.y f-,ractic'lLs
liain II
fl
(t,, De c ncuct=ci
180
Cr DSC c Ut i-vt-
h.-urs
each
)
I OO0 rii.,rl<s:
t - taI ;r,itrk-s ( rrr.:in )
2 CCI
Suo I
tji.-.ch.m-is trY
,' II
tsi. statistics
-.
nr1,.,t* r t''l-1- I 'i c'r t i
&
Granc
5 h- urs
200
'
140o l(larl<s
t ,taI
=-:===--===-:=
.. /.CH,'IIJIxAii
1
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";k/13
i
.\
,t
rl
t
I
---
a__--_-
L_
-
:rl1+n r:,:;
cr
.1
nr**ii
,";;il:;;'"""
(syltaL,us
l.l:.o.J"a)
.,\s1
cctic
re c,,noroi
__.,,r;
;'iQin p-agieq_r_l: jlicr.qL j,q{
,
.T.q1c;n-9_rn).
various critei'ia useci in b;-,cteriar.
classificctic,n. Acvrnccc
n:etiraCs liko ;l_r;.,,c ty; int
scr.o-.t,v; ing Li,.r, ._ hybrlcl
lzation f65
I'p;rrcation or the above in thc
Bercyrs
;.1':.,':""r:::::'::":i".
.r.jcation.
'illent of Fun-a1 rrotozoaf .rnc'vi-.al- class-i..ir.e.ar
in r. .qb-!e.r
iql,
f ) Bcrr yr s i'ianuar.I of J e.Lei.,.iiinart
1ve Bacteriolo< y
2) Funcamontals of B:.cteriology
by A J Salie.
3) iiicrobjo1o1y by FeIczarctal..
'i ) . Funcr amentals of rricrcr-riol011'
by irartin Frobisher
5) Generaf l..licrobjoloUy by
Stanier et al.
j,la.i.n
.
.F:
aF.€:q I
II
.,:
j:!e-g.
19.
a.1. i
.
ic.rc l-. io.r o,qy
prrrnaly Sr:concary cross..
in{ections
enc'or
enous a.nc exoceneous
f.
r
inrection .l:;:::li":",Ii::ti:"
infecticn _. n:an aniri:als insects
soil r.,ater anci fooci . .f,cutc
cnc chronic infections contar.cous
ef,iCort:ic encer:jc ;tnd y anc.rr,jc
infetives. i.loc.le of transr,-issj,on
of cj
contact insestion inocuration
insects ::ffir3'""':ti:;::'::r,".
Liff ercnt typt:s of car-;_ iers -.
henlthy convef esccnt ten.l
I
crery
encl chrr,n j-c carrj-ers
contact car.i.j-crs aDC
I,ori,conical
cclri
jrlurr:
iei-s.
jnf
i,lin
ectivc ctose ID -- 5C. ilin:rr_:a1
Lethal
c ose LL
5O. Bacte::iaen,ia anc, selr ticaen:ia.
Vacdincs
.
concept of bcc,s+_r I
cosrs routes or a.ni,i_str;,tion .cral
1:;;;;.;;;":r;.r]]n...,,
ancr c'isa< vantacres lcG
-,
Dr-t lotio - rryer,ltis anc trg.
Frec'_.i.s1-65-;;,,
f acto;-.s 1-.ailror;enicity
vilulence ancl Infectivity of
f athor e;n.
I isr:arses cause( by clif f r.,.ent
p,ir,io,_ens epi{en roro,,r'
Ii.rao,,,.ac1c - ;
cr iagncsis anc
tr.ratment eE inrccti"", uy sta1,h
irureus stre;..tr:cr_l-yo!.,nes streJ.t l.,neumoniae Bor.r.cteIIa
pertr.;;,.;;",]l.a.rrr.,
tubercLrlosis anci i'r leprae safr=,onella
typhi shig.e1la Cyscntr:i.i;:..
on<i cl0str jc iun tetan
i- ancr c. botur tnr* co.yr.,e ba.cteriunr
ci iJ: hther iae ye,r
s in ia .rr.icket.usiz r cisease,
- [:li"r::.*;:;:; .'::";::;:,:;]T:l;;
IiubelLa jnfluenza Hepatitis
=
rabi<-.s virus anc, HIV
Ccntci
....
I
I
,iI
^-y
-: 2 ?Funi
c'r$cc"'
- supcrf ic ia1 sub cutaneous '''n( systci'lic
anc' tryl'aeca1 c'isecae arnoebiasis rnelai'ia tono;Ia'smosis
i.]
c
;;1 6-Lozc
scrn
ias is
ise .:ses
.
fi:.acl.in.r . ii.,I
I)
2)
in.al
aI
ical l,rijcrobioloiy (Tr'^to voI'.rmcs) by c::uicl:shanl< ct' '
ancl Jayar-am
lr'rec ical- I:'licrobiolou)' b)' r:nanthanarayanan
i.lccl
Fan
i-ckker.
3) I,iiclolriclo'Y bY Zinnser'
lla.{-r. !.cp5:.r. .TV .Irnnunolo.iy
anc. pissive i'iecha'n isr
Typt:s of jrnr un ity inrrater acqui-rec' activc
ofjrrnatejmn,unj.ty-bar;.ic-rs-i.lUCUSol.r(ke'r.atinizcr-sl,.j-n
;.hagccytosisjrrf].arllrLietiottLlumcra]-ilnccelf-n1gcIattc.imillurrjLll
I- i[f '''rt:nt classc s
Brsic st:;uc'Lurc of imr:'uncg)'obl ins ec-i: Ig G'
ty1 es he'p'tens
of itrlr unoglob'-rlins anC f unctions' Anti(rlns
rcactions., Ireoil:iat jon
al-rtit cntjc ceternrjnants lrotigen antibociy
cf an-Li-'en antiboc:y
agr Ir-rtimaticns cI jn j-caI aFI Iica'Lions
anc iTcsturn blot'
i'6:.:ctions e9. rviclal VI'lrL I-iIV te st by ELISI'
thcci'y r'ni cv i('cnc(:
The Ccr c,nl.y acceltecl clonaI sc'-lect ion
antiboc ics' C JIs ci
f cr thr sarn('. I'lonoclonal anc l olyclonal
T iLnc B cclls
irr.,'Ltne sys.Lc,nls - leucocytes lyml'hocyt<:s
cclls cor'r1 Icm'nt systems anc
macroJ'hac'r.: s Fli',sma cclls blast
i'Usfunc.Lions.Hypersensitivity-ciffcren'i,.11'1rr.s.rr::tllttnt:
sGrun sicknesss'
corn;Icx Cisr:ascs cg. Arthus rcaction
anacnia erythroblastosis
/',uto j:lrrnune ciisc asc s f:efDic rous
foetal is rhc-.um;rt6ir-i arthr itis.
,S
qri1c. 9.t e.C.,R
c.qcr,
!,qp.er.
.!J
in5-s-
et al
r. I.,leclic;1 l.'licrobiolor,y (True 'olunres) by cruickshank
ano Jayaram
2. I,r,oc' jcal i:ii'crobiolor 1' by /nanthrmarayanan
Fan ikke r
3. i,licrc.b iolo1 y bY Z inn ser .
fi1a.15r.
.ijlic.r-o.b.i.a,1. ,F.h1's
iclo< y
hlc
of i,utrltic'n - autotrophic l-retcro trcl hic lithotroi
sourccs cf
organotrophic photosynthetic ancl colirlrinations
j.aI.''
f
'struc-Lu:.aI car:bon nitrorcn erncl e nerg'y Cl'o''th actors t':cne
ancivi.i,ar..ins.Cellciivisioncel}cluantitaLionmethocls...,
l'iocrr:s
Contci. . .
.
/-\-a
-: 3 :un i-bs
nephelometry colony or-rrincpackec ce11 gglunre (rcv)
curvcs gene:r'aticn tirrc
(Ci'U) electrUn:c countels' Gror"th
and
bacter i-o1hace nutr it lon
;ind
vi::aI
s
culturc
stt,irc.y state
erirbryoriatcd
ceII rlonolaycr t Iaque countirrg
f
cluantitation. oxy< on
<-'rorvth conclitions ]ike
+ricrocar::iel's
ct-llture
c(,jt s l ikc anacrcl ic
erl:'l-u::o I'll - sI- cc ial ie
::c r1u i;':ctlents ictr;
hylic halophylic baroShylic !ro';'th'
thi:nro;hylic
.ti."-....
1-sychroJ
a
{-,,', n.rrte r i I
by A J SaIIe '
I. Furnc'aire ntals of Eac-L I rololy
aI '
2, liicrob j olot- y by pclc zr'r ct
by i'iartin Frobjsher
3. Funcia.nentals of J'iicrcbiolo!)'
ar
,,j Generar n'icrol-icloly by stanier et
j.la.in. !.ap5.r.
.YLl
FnJ.ir'o'n'm'e'n-"-a'I' 'aFJl' '9'a'n'i't';'!rp'4
]'1i-c;'c'b'io'I'o'tly'
significance jn dieease
its
anci
nicrobiololy
water
I. /til- anc
jrr'rals anc lrumans' Ssn itation of
forecast5ng in plan'"s an
SoiI borne
jnclustrlal anc f ooc' - [ rocessing "'raters'
stucly
anci hunans' I'ie"hoci s of
pathoqens of J Iants ilnirnals
air sonrl:ling by in; ilgment
of thcli-.bove systetr s - eg'
of
techneque ' S;f r' Ioc at ion
f
iltei
s
rncrnbrane
iquc
tcchn
f un'ent'ation units etc '
aniiaal hottses llosr jtals Sriustrial
consj-c'erations' Evj-( 'nce
basec pn aj-r' rnicrcbjoloEical
E' Coli potability rat:c
of feial 1'ollutron of v'irter tests usc of t''{ac
con{ irmccl and com; Iete:c]
tivc
r-jrsumf
I
ancl IIGLB meciium il v'ratc:r
ccnkeys Enco and EiB agar:
r':rirtr-:rs ;rfQccsscs like
er;3lysis. Stancl'rcs of potabl6
ication cisjlfcction by
fIocculation sec' jlren'cation c']i':rif s jnf ict ion
UV C i
'
chlor ine or chlor inc oef ots
c'fflucnts'
anc' trcl'trncnt of int'lustrial
t::citrnent
Sevrage
2.
Seconcalny anc tcILiarY
I;lasto raratcr tlc':trrrcnt -. lrimary
feltcrs
systems Iike tricklinl
trcatment'- usc e1 2161irtcci
contra - etcr ' lnacrcl' ic
ilm
b:of
anc
sluc'9u
c'
act ivate
into
i is jnf ection bef ore c isf osr'I
c ir'cstlon att'a} I oncs
tlc tanks' ConceJ t o{ an cnr'
$,3tcl [6r jos' Lcmestic se1
a l"'l"stc'
the cost of ;urif ic;ttion of
sett:n''
off
lrotuct
Ccntcl ....
t
I
i
I
t
,(c.ec. ifi.9. l,ie:tci.
i:I.
I. Iiicrol-, iolo'.y L'y I'cIcz' r r-'1" a'I
2. Func.imcntals cf iiicrcbjcloly by I'J'cano
jn Frob j'sh< r
3. i:unclantcn'LaIs of i'lic:lc'l i-c'Io1y by irlar-t
i,.tain par.rJ VIlI - I.c.c('. .'rnc. /;qr.ic'qltu.r.ql' !i!cr'c'b'1c.logy
Sourcr s of
i. Fccro si'o j.Iage - tiI ls i rtc' causel ar cnts '
t::t::-:t-:
contar,inrrtions i-n f o''-c s. i :vc-l- p::r scrvatt"" jrtretze "l':.,,^
- ( iryln(clrnn
bcil jni: clry jpr- an( si ILin' 1:r stt'urizi'ticn
anc.Garn,.'airraciaticninftoc'lrtservatit'n'-(!'Iotitois
lr] pt'': servat ivc s j-n f o< c - soc iun
on ion s etc . Usc of cl-rt-r;ic
f its of
i'Le ace:tic a.cic' in J icklr's etc' Dene
mctabisrilf
nicrct'iallyforrnentccifocc'sintormsofnutriticnclircstai'ilit1'
oD{' keel: in i qual itY.
Lescasu s causir' by
2. Ila-LuraI derf ense m'' chun i sms of 1 Iants
iens i 1\rcof Iasr'-a
Pre uConon
as
so
ancl Pe stalolia
tunic f ac
Ianace t.'rurn Acrobactcrun
ntc'Lhot s'
Palmarum ;rncr their control
of carbcn nitro:cn 'rn('
SojI }4icrobiology - elerncnl'al c:-c1c's
anc their bt:r'r f :ts
sulf ur. Rhizos;ht re stur' ie; s' Ilycorrhiza
likc syrbiosis
in a!,rj.culture. Sci l'tjcl'o fl-o::aI associa'ujcns
a.ntar;onjsrn;'at.;.isi-LismI''hizobialanc'12ele!act"r-ialfutili7:cr's
L,liciobiaIp'c'stcon'crolusinqba'cte':ria..'ncvi-ruscsl'licI.cbial
bj]o-ILIr]Cciatjontllrus.iorma.uionanCCarbonSlrqucs-t.ing1q;..c.in,
to 1r:tro - dePosits'
,1f5.a.c.
I.
2,
3.
r,.
i,n
r-
.n1a1tP..r.
i.q1
icr
Foocl l.licrob ioloEy by Fraz
Ray
FocC iiticrobiol'ogy by Bib6rk
Soil iiicrobi" logy Ra'stin Alexander
/rgricultul'al l'licrob joIo"y by tL;ngasr^iami'
J,!a,1P. ,!aP.e.r.
.f{
..
.
I,n-c]r1s.t
r1
1;
I.
.i! ig.r.o.b. 1o.I.o.g.y'
I.In('ustrialfurncntations-uscofbicreacto::-itsst':rilizrti'-'n
cl issolvecl
i t'n:per;''ture - f oan anc
a.cr:,rt-.ion ar:,i-ua't'icn Pli "
Bcncf its by v'ay of
ox,y(jen contrcl Proc:uct ';'c'ntoring '
v'astc tackl in"-' servicc '
L-rioniass chcrnical I'1'oclucts
Contcl
" "
-.. 5 :-
2,
cc cher s
itiona.l. usc f cr J'roce ss in'', surp'Ius f occ '
jcl'.Ic i:al.in''. Tocl syt s rc1 re se ntativc t-icr'c rr -''1.
'r.rin( ancl 1
iIl jn
s
inclustries. lilcoi'ro1 by ye l:s'u f rcI'' t--'olasse Jcnc
clcstxiCittr:' 51 ':
by f e'ncllliun' 51';: 'Acctonc/butanol by
ster<'iCs Vi'Lirr'ljt'
lviiylase by b;:ctr-r ia trlrnsfcrmiL-Licn by
jnl' f cr
g-I2 by strc;tcnyci's Sf l- ' f o':n stre:ai: []'ocess
purification of useful r;ct'abc'fiti-s frcrr: fcrments'
bulk -' 1.6vt -' v; 't
G':nt'raL conbcl-t of. ccnversj-on cf high
ncn - iclishal-tle - hic'h v
1:,erishablcs tc' f srrr vclun'r 'f elmcntations '
I ro(lucts using mi9':obil
Tr'arr
3.Le.ctl-ccultureSarlcyoghurtma}:ingchccscirilk"inq'-rJ;l.n.i.
by r,:icr'-bes str'rc.y cf brcit ir'rkin(-' ''n( icLr battcr'
Lr: i.a.I
.l:,,-:,d,i.n,,, .i,'ia
l. Inc'ustrjal l,.icrcbiclcly by Frtrscott anc Luna
2. Inc ust;.'lrI ir,icrobiclcgy by i'iilIcr ancl Litsky'
3. Llict'chnclogY bY kshar Trchan
.Ll'i:9,
I.
.rlic.r.c.!i,ql. -!9.!.c.tlc.s,.a.nc. .t".n.c't+c. 'qn'-.inc.r.r'in'9
(Prloct
jaI
ical exl lanations to tN'r' 's (,en(tic nat<i:
thyr ine
l,iutc't icn s - s; cntc'ncous an( inouce c' rcle cf
<irne::SlncL},1|.reI,ejI.Cher'icalnuetagcns'-n.i.trouS;.".
rrjlr
nitrogcn nustard . Phys:'ca1 trutar'en - UV' ccnccy't 6'f
typeanuauxotrc;t'hy.Trc'hsfo::rnaticnTransclucticnanc'
of
con jugaticn jl b;''ctcr ja. Il';'' Ir:vcl rci uli'tion
I,rticrcb:-clc,i-t
inc]uc:bIc, anc, CC,hStitutivr eFrZ}Di;S. I,.ecrir[:inr.nt
Il',,..
ti'chnolcay-uS(]ofrcstrictic'nen('cnucleaSl]sI(sc1u.L-irn
cf I I"/. f ragncnts by ac;arc sc e Iectro p.hcne sis anu ce s iun
chlcriclejscrhycnic'ccntirfur,aticn.ccnsrui:tlcticnof
llaslriclvt'.ctol.suSlri.-;lcr,lScslntro...uctj.cncfsuchfcr.:
Lti/,jntcrrc.fic-ntr:icrtbialplantcranirealccl-Isusinr
tr:chnir1u":s 15kc 1-hac'e - tr;'nsciucti<n tranfcrrr'ati-r n in
j-nr chf cliCc cIectrc;oraticn nicrc
I'r€ scncc "',f calu
injr-c'uicnuSCusccfTiPlasmiccfAgrcbact.-.riurr,tumc
.faciensJnplantccllsrilnlsfcrrr.tj.tn.Tra.nsgcniclla.r.L.,
Ccntc:. . . .
x
-: 6 sz. CelI c isru;ti:n te chn iciue s l ikc 6rr-Lan anr ;,c stlc use Ftinclr
f rcss ultra s.nicati'.n anc. fractivnatitn using iscl ycnir
centrifugLticn el-c-'ctrcl hcresis ci ialysis chrcrna'tczra'1 hy
.S.u.9.,_9.
s.t.
{
.\C.q.-_ln i,s-:
I. l1o1e,6ulc:r B j'-lc LY of thc ccnL: by J I r,vats.n c-:t al .
2. B ir.chcnistry by ./r. Lehn inger
3. ltcle'cuI;r B ictnchnc tr';y by Prirorc'so ct aI .
Papcr V -. J,ii.c.rr.bjc.I...ty -lr.qc.!ic.a'L
.E1.a.qr-
.I
Stajninr, Iticrt sc(:ly ancl classif icati':n.
Prc-pilraticn of nutrient a('ar an(' autcclavinq; anc cistrjL ti
in stcrilc ;,1atc s.
2. Isc.latic,n c,f purc culturcs ,;f bactori;i frt t;r s'- iI' usinr; tn'
I.
I.
above
rnc
c
iun
.
s'uairinl anc'mctility tost.
(using hanl; inE clrcp pre;;;ra'ticn) r'n isola-ue s fr'-n: expt '2 cY'
1.) CaJ sule f laEeIla vclutin ancl s1'cre sLain irg usinl a1 1 rc;:: ii
3)
Granrs
c
Il.
f-
)
ulture
s.
i-cal & sonitaticn irrlic::rbitlt(Y.
ut il izat ir-n c f glucc: se f rrrctcse rnann i-t'-:1 ancl lact< se
aciC onc cas ;rcCuctirn.
Ilccr
2) Ii,'U ic te st s .
3) Isl Iatitn cf actcric;hag''
paper XI
f r'' m cevragc
.l,iic-rcbh.I.S.';y...qr.a.cj.i9.9.I-
.E.x.a.qs.
ft
'
.IJ
8, InCustrlal I''iicrr I ir 1:1;
r)
2)
3)
-Ep.y.g.c.n.q9.qt.er] AgrlclrlturaL
Usc cf liarc Ccnkeyr s l,nc< anc Eits agar '
.
Lc'i:c'rlritin:;,ti-cn c'f eff icicy cf becterir Fr'-'-f - f iltcr
crt v'c'
Ccml:,ariiscn cf air -' f lcla by plrte enJ'Ic sur-e in
un crc \rclccl Lc c at icn s '
sar" I
Fc r-sunr'tivc inc' c'.nf irrnci tcst '-n "'cl1 - 'rrtlr
jsh'
IslIai;irn c,f s1'c ilers frcnt clccayin'; f
Is. lati,.n cf r"-: t :- 1-1'-6sler brc'Lcria'
t\lcsguitcciclal off icacy (f Ba.cill,crs thurrinr-. iensrs cu.l
anc.l
r,)
5)
6)
7)
B)
e)
Isrlati'-ncfcelJul-oseCiEcstinib::cte:rj'afr:r'.lcr-r;6"1"'
llcttinq; <:f ,.rc'";th curve r-f acr:rtcc' culture <.f bact'--r
r r yc l'.st.
C.-
ntc
.
i
I
I
t
[.
.-
-.'7
IC,) Fumcntltirn rf.carsh:,' a1 1Ic juict'intc alcch;I us:'-niyoa,st
rl) rsilatirn cf micrr bos fr'm rcri batter.
LZ) L^r;r.nstrati.:n cf r.ntibi:tie Ir.cucers fr<.n sc iI using
, ,il lk in s
er r vr lI e:y chn iquc .
a,'-,
-1,
,
I
tt
ros/20,7
,h
.
f
I
I
,H
B.Sc. (Sr.bs_jrlia-ry) elCCi._;tIS.IIY
SCHLI;[-. CF Eht\, ]l.,.TICN
Paper
f u::at ion ,, ye ar
.
Subsicliary: BICCIj;.-t,iISi.iy ir.ij-IrI 3ilCUitS 1yE,.,i.i,
Sub s icj iary: E ICCI-lEj.iISTRy Fr1,PF-R. II
3 HCUiTS II yLiJt
Pr act ic al_
ll,ecorcr s
Iiotirrs
'IOT/:J- Fcrlr
SUBSII lAiiy
il Yllii
L.'lar-k s
6C
6C
60i
AC
200
SYt,L}BLiS
TtiEC[iY P/ip-pi
F
IIIST YI-;rIt
1
'TCliL
')
1. Physicaf'A.spects:
Futrcl
atnetrlrrl
I,t- jrrr:.iy,'i-ers
i,,t.lii(S 6O
TCTI.L TIl.L. 78 tro,rrs.
26 hours.
of Ire
[,tJ
[sr1.rj.y.1,-.,g
of r0ass ac r.ion s-i: ;,ten*.n1-s anci explanations, trre ec,uiribrium
constant o'i ions, cJectrolytr,:s ano norr ef ec-Lrolytcs
Brons-Lecr s
de{' jn jt jon rrf acicl s anc' Darr.i.meaninq
normality, molari_t1r,
^of
morarll'rr, ac'Luar- aclci'uy and*-irable acicity
of sor-utions:
. I ir,r :. .. .;;' - :.r c,f i i..i.:.., ,ra,n"nf,r= o1
ancl
pCll
nI
. Sin:plc ni_rrcric;, I
probI,,ms jnvolvinc cletrmination of H+, OH- pH ancr
,CH.
Lar'r
Erementary study of nnethoci s to de.i:e:nirre the
F.Hra) theor-e,t._ica1
cal-cul-a'Lions, b) using inct icators inci e)
usinq pH mn-Le ,-..
L issocia'Lion of r''eal< ac:cls Hencersr.rn
Hassefbach ecluati(,n sir,ri.lc
numerical problenrs invol-ving ap;.1;ca-ion of .this et
ua.t,..c.rn.
shhpes of ':itra'Lion curvc s of strong ,.nd yeak
ac ici s ancr b., sc s.
L{eanjnc of. (a anr, pl(a v;-rl-ucs. Buf.,i-crs anc,
buffer actioris. 1iinrr,,l
numericaf prol le rns on buffer compos j--t-ion.
Funclarieni-al princi;,rles of <jiffusion ancl
osmosis_ Ccf jnj.Lton oi
o smo'Li-c pl'c ssurc rela'c ion ship o f
osmot ic pre ssuie io ,: as r- a,, .s .
The c'erreral ec;ua-Lion f or- Cllutc solutio4s ( p_g/m
lrT).
Probl-ens invorving the. eene ::ar ec,uation jnf f ucrence
of ion izal,uic.
anc ncl.ccular srze on osmotic pressure. Func;rr,rcn-caf
s,i.uciir 61
I onnan ecluiribrium' and ci.ial,ri-c. Lieaninc, of colloic.af
soru,cions
ano col--r-o i-c s . Llcm,rntary stucy of charc e on cof
l-o ii s
effcct-' f)rophif,.c an: r,'-,-irobic coLloics. trnulsions
enuf sif 1,ing, ar,cn.Ls,.
anc, tync
anc.r
all
-,
.4) -
rr'
Pertinent a..l';r r. I s or' crcan ic che mistry; (.0 rro,.ri,.s
)
reactions of the fc1lc,,r,jng functjrnal croups: _Ci I, -_SIl
-CFIO, {{,
{CCH, ald -lril-i2 groups. ctassification or. isoncris
v,tith examplss. Basrc. jc. r, as altorr-l h)roro!en bonds
.Chernica_:
Ah EXample c.ch from orcian -lc chcnistry f or the f of
.in<, typc : i
-tor
re:;lctions-substi'Lion, accr j r--i-on, erinrination, condensati_.n anr
dec arboxyl_ at ion
tII.
Cheur
istry of carbohlzcrate s3
(
l2 hours)
fsomerism of car-bohyctlates, relationslrip I anc. L fOrms cf
qllyceriilcehyde- examples of e pimc;rs_ mr.r.Laro La-,_iorr ancl i,cs
.
expranat joh by r:nE st-r'rrc.turrosr,. orlorncr-s-st::Lrc.Ludres (linear
..,nc
cyclic) of c,1ucosc, calac-uoSe, fiiannose c.:nc fruc.Lose. rrf;or+"ant.
chemical reac-Lions of clucose apci fi-uctose. str-uctur-e of ncthrrr
t qlucopyranose and 2-ceoxy-B-iJ-ril-,of u1an,rse.
_t
Structure of the {oflorujrlc. c!isaccharic,er,Flaworth i,,oc)61s) mafio:.
isomal'l-ose, sucr.,se, 1ac'cose .jrlci celLobiose, (l ]uciCat jon of t.hrs-Lructurcs of nrono- c; i- r.nc) 1,o11,s2."1-,.ric e s is not inclu6sq .
;
structurc and inpo:-La.n-L proper:"ic s of the fol-lor.,inc 1:o1yslcc5ar;
amylose, am),l.opectJn, glyco en, ccllul_ose ancl chitin (f -.1-,,rcicl a-rir
of structure of polysaccharice is not lnclucied).
IV. Chemistry of Lipiiq
(e hours).
Basic icls6. about physioroi icaL f uncrions of ripic s. F;,,t,,.1i ac jr
classif icatioh. S'bructure of thc -Io1lo,,inc, f atL), arc j_c':: srrc.:,..
acid, ol-eic acici , linoleic acic. sr;l'r:c-'uure of tliElyccr.ir_cs,
saponificationr. structurc of phospha'Lic.io acic', lecithin,
cepharin, anc phosphatidylserinc, lo e f ini-L,ion of sailon jf j.cation
number, ac io number ancl iocl inc n.lmbt r of f arts. ch( i-nic af s.Lructr_,
of choles'Lerol- anci ergostcrcl.' (Llucldation of structures not
inc lrjdcci ) .
V. Chcmistpy of arnjno acic.s. and pro-Leins (-t: hours)
Basic ioeas abor-rt phl,sioloc icaf f r.rnctions of prote jns- namcs anc'
s'l-::uctur-s of the 20 important r.r,rinoacicis occurina in
1_,rotc insqeneraf chemical- reactions of arnirroac iis. Ilcprcscntatlon of
af an ilr: in 'i-hc Zr..,rL'uer jcn or:.r.
:1.
Elementrry s'uucy of primar),,
s-i.r-uc'L:trr
s(
conoary terti-ary anc
611_r;;.rcrnary
of 1-rot;:lrts, Thr: clcn-.-al- n:thocl s of protcolvsis,
')
o, ,,r.-j"i.c acic,s.: (6 hours).
C1a:;s -j.i i- ,'L ion c f nticl-c i-c .c icl s , cl'irlrrlic aI structure s r-,f ad:'n itrr: '
gtranJne, cytoslrtr:, urac il ahd thymine. Structure o I B-I 'iiboturinrie ancl 2..deoxy-B*l--r:ibofuranose. Struc.Lui'c <-,f aclcnos,
anr. ,i,'rP. Structure' ,, ^.;.,, ir i.r,,aiiy anc se corrciai:y st;.uc ltti:c oi
Llri/., regul-arities in b;sc co!-,tros,i1-j.one. The lrjatsoo.rr i(lCr,icl: I
of Ll.l/. (phytrologicaL ro-l.e of nitcleic acids r,,:il-l- be't..ruc,ht onll'
in the s-ccond ye ar )
VII. Hermone s: (.i hours).
The site of biosynthesis anci chinrlcal- s!r:ucturcs of ti: f ol-l-ot:i''
hormoncs: thyroxinr, insulil, ccdtisoal,. coll-uisoI, ac'l-cnal-in.,
II- cieoxycor'c ico st.cro icl , aldo steronc , tc' sto stcrone , e s'i-r'onc ,
e stract iol anC ltyplrophysc al horntone s ( v.'i Lhout s'Lruc'Lu , ' ' n I
hypopyscal hormonc s). (Sturdy' oi y:h1'sic:logicaI lol-c o- Itc,::monc "
is expecti,ci enly in -bhe econC yoar).
Vf.
Chemis-1r1,
.s.LccriiTOTT.L I'U'IiKS
I.
6C
Y!/fl
*. lhE!"RY .r,'_,F_Fs..Ii
]'CT,|.L
Tli li '(,
I:CUliS
(20 I'iLUIiS): Lefjni.t-ic.n of the six bas-ic r.:11-,s*ccs cf
s r^i lth one e xamf ,f c ac h .
El-ernentary stugy of the f o1J or',ing f actors af f ecting '16ler; i'l-1, 6'1
c.nzymci catalyzec reaa'rions: .. cf l-.e c'c of enzyme colrcettt,rai-icu r
t-emper-atur:e 8, pl{ eff ect of substrat.^ concern'Lration- ',ichacl- i'r
-iVienten e(.ua-Li-on (vritirou-L c:,riv.ation)- The Ljncv,eavet L Burl. pict'
-tef initioh of k_^ \';luc s of an enzymo.
Lefin:'ion of en2yme Fpcc-ificil:y: in example cach for crol:;'
spccil rcit.,, opt'ica1 spccr . icil.y, <e oma'LricaI 'slteclf :c j-[y, anc
co-f actor spec if ic it,f of enzymc s f rom the pathtvays to bt sturi 1,this ycar. [,xp1anatlon. of competctlve ancj ncnconrpete';rvc 'uy1-rc
inhibit j-on - the ir c i: L h;tiron on the basis of doub-Io lc. c; ir:rocl
plot-bre if s-Luciy of a.ll ostcr jc inh jbition urith an cXe6rrl6 .
Ei,iZYlvri:S
en zynle
r.:
a
BreOf 'stuciy of the actlvatron of zyu'o( cn f orm of elrz\rnr,, ':
r.ct ivalion of -SH en zyme s ,. ancl act iv at ion of en zl,mc s by
prosthctic r';roups, coenzym.is, airc. n.taI activators. Bri:f
strr,.
of allostcric ac-Li-va.-Lion r;.,i'ch exarnple .
Structule of -Lhc f oll-or';lnE cocnz).mes--l'i,. +, i\;.1. F'i , Fi,]., F,J ,
Iipoic acid, TPP, Pl,ricoxal phos;,hatc , p)'ricloxam jne phosl'lr;rt.--:
and biot:1 . I''ietal oljc reactions (r'rom-Lhos,. larc.rscl'jbcc for sti:.
r.rhere these,coenzymcs p;r-Lici5::'l-e-- or€ examl-. -]-e each (nlciranisrn
of action not e xpcct.cci ) .
II. lnaerobic motabol-ism of car)ohycliates; (Il hours).
I iciestion of calbohycratcs ,nc, rbsorption-rcactions of
glycolytic sc-:c1ue nce s vr j-Lh namc of cnzync s anc jnl:'. 1:mec' j rtds
(r'rjthouL s'cructures) f atc cf pyruva-Lr: in af cohollc f ( r-.mcn+-atic,i'
4-;"liP anC.[crrnoncs in
c'1ycr).,e l.] m€ta])oI jsnr*c,lucon€i ocant:sirj iJtC
pe'i-in'ray
(orrry ou-LLincs urr;hc,uL str-rrc.i.rr;.r s of
i..entosc
''_nterm, .j.phosph.tc
iate s. )
l.l-, I,,'iuscular contraction! ( 3 hours).
,\rame of djfiercnt proteins in *r.. l,"s-the sIiding frl-.r,-nL
i.heory-eryerc,y sources f o:- riusc,rLr i- coil'ur-.,ct jon.
TV, Aercbic oxi.dation of caibohyc,r-atcs anc.i bioenei-c,.-,ir..S,:
ir2- hours).
l egg,cboi,<Vl ;-Lion of py.r-uvat.,-.rc'actions of c itr j.c rrc jc cvi l:
I.'r' j-thout stluctures
of in-Lermcci ia.t.e s-onJ.y or-r'Ll jls cx;,,:c Lcc ) .
,-,afculation o{t.l.TP')iiolo oi anaerol-:rc
ani' :,,..robic ,xlcrirt jsn .r
r:arbohycrates. Thc r,ritocl-ronclria -arrancc:n;nt o.f el_,_ctr.cn
c::rr
rn thc el-ectron transport cl-rain, sLltrctrlrel of /-Jp- sub: ir.r-[c
.l
1'hosphorylation-sit<' o[ ,-.TF f orriat:on j-n -,he chain (m.-chin i sm r
r"IP formation rrot expccted). crassif ication of hich-ene r,;y
compounci urith an exanrple c ach.
i,/" l'ho-Losynthesis; (+ hcurs).
I
,
r-.utrjne 9i cycl-i-c anc. non-.cyc1rc photopl-rosphoryfation. oul.l_inc
stuciy (wi'Lhout structu:-es) of i-he piuh of tarbon in -uhc. c.-r-k
.caction (CaIvin C),cf€ ).
i'I.
of J. iprc s: (< houns).
(;ut.I jle s'uuciy oi l ipio i j-..,c:,tion ancr absorp-t iorr. C'u t-L in, s Lut
It-oxicl ation schcrnc (r,itllout str.urc-Lurcs). ,,tp yiclc: jrr g..
iixida-u:on-outlinc stuoy(rrj.i-i16u{ structures) of 'Lhe cytopl-;smic
''v: Lct'rs of f at..i-1, acicl )iosynthe sis. physiolocical_ f .rnctrons.c:{
Ivietabol isrn
.
ph<, spho
ir'If.
I ip icl s .
of anint-r aci-c s: (: hours).
Prote o11,'uic enzymr.'s of thc qastro jntc stinal trac-L ancl Lhe 1::
activation ( f ronr Zy:to< en f oims). ,,bsorption of chino ac icl s f r-:.
r-f,e intestine - an exat:npIc each inclica'uinq c,ecari_,oxy'I it, ic,n,
c,c'amination and 'ciansamination of arn-no ic j., s (r,rf1i-.rout ml jr crrl
[it:-Labo]-ism
mechanlsms)
\rfII.
of netabol, ism: (f Z frours) .
f he chcmical nature of genc-rcpl lcation of L1,,,, Cocl
f or ar,.: jnc
-i:f 11 q
rc icls by trip,lct.s of b.:ies anc' transcription- of
,.. = f:il" iir;;;
of IttJAr.thc,i:: sti'rrctu::;r1 fcaturi.s, ther-c r-ol-e ii-r protcin
.typu.
) iosynthesis (func.arnerr1.a1 sLucy), tlansiat jon of m,illr:., i.lrc
.t'ibo somal cvents ( cl iac'ranat 1c study on 1y ) - polysome s .
Gcne-Lic aspects
----'\
^.-\
ta
ct
( lrk^-s)
d;.1'I;'i';?.1l;ili:,'i itii,,,l'1":,
i,l:,'-;t ,il.llf:."i1";].,,.;ri;.
r.
. ,.
y.^ I-i()r:.,)'rcs! (4
hours).
l,letaboi of f cct
gf
, insul in, glucagon.
.io'.,,th h6r*un.-,
on ir,n ,u u.ujr!P'!li,i;"'3:l
:iX:::l'.i".icr] una,thVro1,:nJ
.
,
xJ.
Tr:r''."-
lLements: (z lrours).
the sysr-6sl5 afri_.ady s:Lucir 5 1r,l1s1e {_..6,
nts .,ive a rol_c . (lxe;;a;ir"
or ac,.ion not cxpre:1,,6,)
lie Labr:1jc reactions
f rorn.
c'.1-cme
,
fi i',i.
ii,
ii;,**q
i.y; ..
i= H;
'rotaf marks:
60, records: 2o nerks.
Gu
3'?
ant
lt
ia-.
;
ive
e
:ji * 78 hrs.).
TC'1'rI
T l',,i1-_l 56
s-ulmat ion s:
j. Gj-ucos: by
/\r.scnomolybc,ate co.lorinelI'lr
mcthocl .
2' Glucose by Benccictrs quantitativc
rcacent.
li
,.nrjrro a:ics and proteinsS
C<uant
1.
)
a
i,t
jt:t ive e stlmation s:
Gl)'.- inc by
P-ri-; l-e
Pro
/-
-:e
-,t
binhydrin reaction
ir by Biur;t nr--thocl
in by Lo,,vryl me-Lhoc
'rl t
R;-oe/t,r".s
( t'alt
,.<L^t7-t.
ci/
3
b"u
a'u'et
,.
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