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cctic T.a4c.n-ol:1, criteri.a in
,4 .SS... j,llcr,o.F.1p-Io-Ey
l:11:1.
anc As; cctic
.P.a:;'.c.q.-J GeneraJ i',iicrobioIoE;y
(
Syf I abus i.r i:enCecl )
Te
clrnolor
l-qie.a-f,r: Jiic.r.qLli,al T.a4c.n-ol:1,
various criteri.a usecr in bacterial cLassification. Acv.^nc.
nethocs Liko phe,-e ty1 ini sero-tyi- jng LLl,.- hybridization I
ribo soile s-tuclie.s etc . lrnr jcation of the above jn the Bcr
rianrral. Broac t::ea-bment'of Funr at rrqto zoal anc' v jral_ clas
fjcation.
j.ia.lq
,Fte.q,.in
2
c_.
.qqte.r iqI.
I ) Berg,yt s :'.ianual- of I e-Leiil:ina:tlvc Bacteriology
2) Funian'ientals o-i Bac'r-eriology by A J Salie,
3) iiicrobiolol,y by Felczaretql.
'j ) -- Funcamentals of i'iicrcbiofo:). by i.iartin Frobisher
5)
GeneraL t:ilcrobiology by
Stanier et al.
j,ia.j.n .F:+:e:q -LiI. .-: ;t!e919,a.1.
f ,ic.5g.t-.i.o.I.o.qy
Primaly
scrccnc
ary cross .. inf.,ections nosoccmial in jections
infection ciffq'rent soLlrces of
jon
ilf.ect
- n: an an.rrnal s jn sects so iL r.,rater ancr f ood. I.cuto
anc chronic jnfe'ctlms contaceous epiclet:ic encesic an4 an6'e :,,
i
enr-orenous ano exocGneous
jnfeti-ves.
:',iocle 01'
tranSr.'riSsion
of Ciseases rg.
jnhalatj_on
contact ingestion inoculation insects congenial ancr i:.itrocenj.;
Different typc's of cari'iers ., he&Ithy convalescent temporary
anci chrc n j-c carriers contact carricrs anc f:oriice[ ical carr j_ers.
i-iininiura infectlvc dose rD -- 5c l,l:-nirnar lethar c,ose LL
50. Ba.cter'iaemia a.nc' septicaemia. vace jnes conce;t of boc,s-ter:
cose s routes of acimin jstr;rtion - oral Farenterar
- acvantare s
anci c j sac.vantagies BCG I FT IoI io - riryet j.tis and TAB. prectispc: jr
f actors pathor;enicity virulence anc rnfectivity of pathol,en
"
I. ise ase s c auso( by cl j-f f r.rent patlro, ens cF.ic en ioloi;y syrnp.tonr. -r rlc
cliagnosis anc trcatnent eE infections by staph aureus streJ:tcro.
) yofenes strept Fneurjloniae Borr,cteLla pertusis I,Iycobacteriun:
tubercr-rlosis ancl j'l leprae salnonerla typhi shigerla. cysenteri_,,
ancj clostrjciun tetanj. ancl C.'botuJ jnurn Coryne bacterium
diphtheriae Yc,r.sinia - l.estis Leptosr-,ira icterohaemorrhagiae
ir.icket'csia.L ci.ssases - smarr pox chicken Fox mumps and measles
Iiubella jnfluenza l-iepatitis rabics virus anc, FIfV
,
Contci. . . .
Fungall c r'-seaises superf ic ial sub cutaneous an(' systeinic m$c,
I - .tc 2.,''. '-'i-sea$e arnoebiadis rnalaria tonop.lasmosis anc' tryf
scrniasis.
!qc-a,c.lp...
.,
i.tt
i;na-1.
I) i./tec,icaI t,lj.crcbio1o,.;1, (Tr.^ro voI,-:mes) by cruickshank et
2) i',iec icaf i'r,icrobiolc!)' b), -r\nan'chanarayanan ancl Jayaram
a]-
,
f an j-c kkc;: .
3) iijc::ob,icloi.y by Zjnnser,
f
;d,11l .pap.e,.r
.IV
.!rurrunc.Lo.r.y
Typos of irnr,unity jnn:rtc acqujrec'activc ano passive i;Techanisr.
of jnil.r.te imniunity - bar,-'j-crs .. i:-rucus anc koratinizccl skj-n
;'haloc)rtosis inf 1an:r,ratlon Flumcraf alnc ce LI - rnec'iatccl irnirrunitlr
B:,sic struc-Lurc of imr.r.incc;loblins e!:: 1g G. L if f crcnt classes
of j.r':t'unogloi;,rIins anc functions. Hn-Li_cr:ns ty;es haptens
ah'c j r n'c jc Ce terr:rinants i\ntir en antibocly rcactions F,ree ipia-bior
air_ I rt+u imat icn s c 1i.n ic aI aFI I ic at ions cf, an'L i.i on ant iboc,y
,t(,;.ictlons e!. rvic,al VI.FiL FiJV te.st by ELIS.'. and'-Vesturn blot.
The Cc- : r,.i;"; ;cce;tecl cl-onal- scl-ection thcory anc, eviclcnCc
f cr th. ianc . Ilonoclonbl anc. t,olyclonal an'ciboc ies. CcIIs ;'
-,','UIlc Sys l.,,lriS - Jeuc.OcyteS Iymp,hoCyte s T anC B ccIIS
n-llcroi'har.c .i F1i:sma cclls blast ccll-s cot_lfrlemr nt systems and
its f 'rnc-Llon s. Flyper scnsitivity - c iffcren-L'11'po s. Iri-:n,.r;nr,.
coril;Icx Cisci. scs e g, l.rthus roaction scrum sicknessg.
,",'.lto j-rr;nunc ciist a.sc s Fe rnic rous anacr:ria erythrobLastosis
f oetaf is rhc,urn;rto icl arthr'it is.
er
,S
u.gi.1c, 9,t e.9r, J1c. aic-ing_s_
r.
2.
i,leclical i',li.crobiology (Tlro volunre s) by cruickshank et aI
1\'!oc' jcaI i,iicroblololi, by Inanthrmarayanan aprc J,ayararn
fan ikker
3.
:
ila.in
iicrc,biolo! y by Zinnser.
!,a5.e
l
V,I
.Flnyqiclcr y
i,ioc'os of' |,utriticirr - autotrophic hetero tro6:hic Lithotrol,hic
cr'ganotrop hic photosynthetic c.nq corlb jnations sourccs of
struc-Lula] c c.rbon n itroc e n ancl e nercJy cro,,,th f actors rile DO j-aIs
anci v"'i.-.:,ins. Ce11 division ce11 ciuantita'[ion methoCs
Contcl
....
-: 3 :packec cell- volunie (rcv1 nephelornetry colony forriring units
(CFU) el-ectft,n jc countors. Grol,rth curves gener.aticn time
str,:icy state ctilture s vi::a] ancl bacteriophage nutrition and,
cluantitation - ce-rl monola.ycr plaque countlng embryonatei
e!.; cLrrture riiicrocarriers Eror,vth concrtions like oxy(.en
rocllLl j::'ements teni erat,urc lll
srcc ial jties like anaercl.ic
tht"rrno;hy1ic irsychro;'hyr ir harop,hylic barophylic growth.
I:.q,
g!. rIr
.,
rn
ar
t e :: i
a1
I. Func:amentals of Bact - rolo-cy by A J Salle.
2. l.ticrobioLor y b), EeIc, ar ct ai.
3. Funoamentals of Liicr. biologl/'by. I,lartin Frobisher
,r) General rnicrot,icloly 'by Stanier et aI
-l,lqjn Lap.e.r. "t{fL
I.
__L.n*v^1qo.nqe=njc.a.1.
-a.n_cr.s.qlit_a-!:.on. l,1ij:_rob |o.}.g-qy
jn d iae ase
forecasting in plan'"s an jrnals anc humans. San itation of
inc)ustrial an( foocl - prbcessjn! rvalnrr. SoiI borne
p'athogens of J lants a4;rnals ancr humans. I.,iethocis of stucly
of thc a.borre sys+'e1FS ,l ug. air sor-nJ:rlng, b'y irl1 ingment
Ai-r
anc- r,',rater r.iicrob
iology anc its
s
ign
if
ic ance
il
tcchn iquc s rne rabrane f ilter teehnegu€ . Saf r: Ioc at ion of
anlnal- houses hosrjtals jRciustrial furnentation units etc.
basec on air rnicrcbiological, consj_Gerations. Evic-ence
of fecal p,ollutlon of vr;ttqr - E. Coli potability rat:o
6.orsumi'tive conf irmerd and comFl-etecl test,s use of Mac
ccnkeys Enc.o anc g;'g aEar ancl I3GI,B rnecium jn rvatc:r
rriSlysis. Stanc.;ircis gf po$able lvaters processes ]ikc
flccculation sec i:ren'cation -cleirif ication Cisinfcction by
chLor ine or chl or jJre elepot.s UV d j.s jnf ict ion ,
2.
trc i^triient ani trc-itrncnt of jnclustrial af f Iuants.
I'Jasto r^,ate i trc,atrirent -. f'ri-mary Seconc alny anc ter-biary
tre atment -- 'Lrsci 'af ai,rrted systems I ike tr i.ckl ing iil'!c:.:actlvate c slut'gu anc b iof ilm - con-t:ra - etor. lrtacrcb ic
c igestion algaI Ioncs c]isjnfection before cisposal jnto
,.',ratcr boc ios. Lori,est.ic se1 tic tanks. Concel:t of an oncr
I ror uct of f set-L rh,, the co st pf pur if ic art ion o f a trraste .
Ser,vace
Con-1c]
....
-: 6 !-
2. CeIt cisruJ-tirn te chniqucs like n'icr-Lan anc ;estlc usc Fi
using; isci ycn:'
Frcss ultra scnicari,-n .lnL. fractlvnaticn
centrif ugaticn e }cctrci::hcresis cl iaJ'ysis chror:ratczr..I 1y"
.S.q
q
q19. s.t c
.I!g.qc-. in.1..
s-
i
I. i',iclecular Bi'-Ic!)' cf thc cenii by J I- lvatsc'n Et aI '
2. B ir,chenistrY bY i'.. ichn inge'r
3, ;\tcIe cul ar B ic'c^cilnc I: 1y by Fr irnrcrsc of al '
Papcr V
.[.
I.
-:
jli1c.r.:,b i c-"J,.c.;,r' -Pr.ac.! ic.a,I. .E.4ap.-.I
ing I"'ticr( sc (]j' y anC' cllssificatiln.
'':
j-cn-L a(..r anc, autcclavinE; anc cistrjlPre parat icn of nutr
S-ta
jn
jn stcr ile
5'latc s '
2. Isc,laticn c,f Pu,rc culture s cf bacter ia f rcm s'; il
above
nne
c
us
ilq;
irrn.
3) Grams staininc an('m:tility test'
(:I) isclal-ues f rr.m expt ^2 ''''
( using hernq;ing circp prererarticn)
t,) capsulc fIaEcIIa vclutin a.nc.sicro stainjnE usin'-- alJ'rcii:
c
uIt,-:le
s.
& sanitaticn ivlj.cr. bit-1c q'y.
ut rl j-zat itn cf. i,1ucc,se f ructc,se nann itcl
j-'- n .
a.c iC an( c as l^rc c uct
2) Ii'lV ic te sts.
3) Ist 1a.t i': n c f ac-Lt '' icl hag" f r'' n se vJage '
Il.
I)
i.{cclica.I
ancl' Lactc se: f t
--
Paper XI
8' Inclustr j-a1 I'[icrc l-'ir']c
!,.!.v.ir.c-n-m-el1tal; Agr icultural
Use cf li,lac Ccnk€)'r s Encc anc Eits agar'
Ey
I)
jr Prccf - f ilter.
2) te-i.ermjnaticn cf eff j-c;cy c,f bacter
3) Ccml-^,ariscn cf ai -' flcra by plarte enplcsulle in Qlct4ic'
ancl un crcwclecl l-cc a.ti.cns.
F'crsunrtivc inc c'.-nf irncc -bcst rn r"cII - "ratrr sanrpl: '
5) Is:Ia,i.i:n c,f si'c ilers frin cccayipl' f lsh'
6) Is:]aticn cf :-^'-.. '- r,'':uI'' b:'c-Lcria'
cltr'
7)- rr.icsquitcciclarl eff icacrrr 1f Ba.ciIIcrs thurinqliensrs
cl'-''-'
B) IsrIa-Li,,n r-f ce 1]i-iJqsc Cilcstjnr b:rcteria fr:n' cclil
ie) Ilc.tting; : f , rc,.itlt curve ,-'f ;ierrrtccl culture of ba'cter
,",)
'-.1-
.'-.-c* Jv.
y\
Ccn'Lcl
.-.
1,V
-: 7 ijntc alcc'hcI
IO) Fur.:cnt;ti'-n cf ca'shi'' a1:1Ic juict''
II) Isr 1;:tiin cf nicr'bcs frr rn liIi batte'r'
frtn s'iI
I2) Ir"I,:iDst::at-'j':n cf r'ntibl':-Lie Ir'cucers
-L
''riIlkj-trs ir(,l.r ('v(lrIay chnique'
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us inr Yc Cst
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