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ii:I :l
IJIVIVER,-TT. OE
C.\LTCUT
(Abstract)
fmplementation of the
{ rocational.) attachecl scheme an.i syllabus of tsSc Biotechnc_lo,ryv
'1r
re )9 admission on,firds a" pa: Botany course rvith effect
fro-,.
- orrJers issued.
\\rD tCA)nj{IC J J ,=arro*
Dare'r, ca]icrrr rrni.ver.si-ry,
?. o. 27.10. ss.
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.Science held on u. BIB$:t's of meetlng of nacultrz of
+)
:;'i;:r8i;xi';""t",""rior in the ri.re No, cdrftL/+7s6/ss
"
'
or Board
93?E3
provisional
,vas grante.d virle
*;i
above f or sr arri-n affililgion
rl,o.
s ;.. -:;
( *"it
r;;"
iJ, _ ii"..,."
:3iiJ:, "i:.i*;;; ;;; irre"t acaa".iJ'vear
leee-20:ro"ff:s:.:f..rr.
in sNq,s
technoloq-y a! its meeting
held
Ced the_ i"quf.tiJnl
scheee anci
urse (.,/ocat j.ona-L) .ttacfrJr
io..
.f;: ,rIX;":.:8.;5":5 ;;l:lsir3!. lr.s meerj.ns held on u.In, n, approvbd
5'
rn view
ff yo"n"y, the Tice-3hahcel]o..exerci..sing the po_
ffiI'.:f,ff:S"H:
caricuL=u.,ii"rrit,
;ilff:l Ii*XrHt:iH.::
r
the
t"n,
a,;";;;i;"ili"
Ii#*::
fect from
1999 admission
9
l)
imcl-e,-nentd,nq
;cheme and
syllabus is
1
oq'
aoponde.t.
sd/_
R'I]GT S"RAR
TO
?he pl.i.ncipa.l-,
Of the cbllege offerinq
BSc Bioteehnologry cour.se.
go-PI'
,contror]-er
!g
Examj-nat ions/GAI F, scct,ic
fnformation ofcenrre/un
n/ universlty
i ve isi ty - r,i
rr.J*) r, r
Fc.
RDER
,s
.
P.
2)/to
/
\.tt?
\
--at---;
!
t
-+-
B.Sc. Biotechnology
There wiII be eight theory and three practical papc-rs
for the examination. The orCr-'r of pap.:rs, duration of
examination and marks allotted arL- as follows.
duration
Paper I. Biophysics, Biostatistj-cs, first
3 hours
and ComP\:ters.
.Paper 2. Biochemistry
Sccond 3 hours
rr
rt
General
Papt=r
Microbiology
.3.
Paper 4. Environmental Biotcch,,
nology
Paper 5. CeII Biology and genetics ' Third 3 hours
Pag:r. 6 l,lilecular Biology and
Name
of the theorv DapL,r
vear
rr
rr
rL.combj-nant DNA technology
Paper 7 . Immunologty and Animal CeII
Pape'r B.
?
technology.
Plant tissue culture and
Biot-.chnolcgy
rr
100
100
100
100
100
rr
100
"
loo
100
o
Total for theory=
,..
Name
marks
BOO
=======
of thc. Practicals
Paper 1, Biochemical and Microbiological techniques
Paper z. 'trlethods in cellular and
Biolcgy
l,lolecuIar
?-)
Paper 3. Immunoloqical methods and
vcar
hours
Second
12 hours
BO
Third
12 hours
B0
marks
Third
12 hours BO
cultuie't-.chniques
15
Report based on educaticnal visit
Laboratoty Records: (to be equally
distributed as 15 marks "ach for
the above three PaPrrs)
---31-3O0
Total fcr trractical
f fOO
a.ld practical)
Total fcr I'{ain (theorv
-r(
KGlLzTe/
.-ir''';;
.4.
*.
.
-i
s-IL Ir4UIg
i -x : : 9.__? lgr:. cJry g! g g: _(rt i Iw)
Paper.r - BroPHvsr:s, rlrosT\Tr;?rc5 \"rD COMPIJ:PER9
-q9
: .. 60 Hrs
BIOPHI'
i
I
I
STC S:
Energetics cf living bc.ly: High energy an.l low ener?y 15
phosphate compounCs.
Lambert-Beer law, sDectrophotometry an-1 eolrri.netry, frame
photomeLryr physical metho.ls of fin.i cut nolecul_ar
structure:
Microtechniques i-n bi-oIoqir. principles of
separatj-on techniques l1ke electrophoresls, nrecipitaticn,
iialysism, chromatography, TLC, col_umn affinity etc,
I
Hr.s
I
Intro'luetion to imagj-ng techniclr:es, e.g. ultrasoun.1,
X-ray, 'ICG/ EIG, NMR etc.
RIo-?.1_:1-I_,tEL9s ,
Set theory,- Procerties of subsetg:
Definition of sets, fi-nite sets, countabry irfinite
sets,
uneountable sets anl exanples. Set cner.:ti_ons, ullons,
i-ntersectj-cns, com::lementatiof,.
35 hrs.
Llnear an.l geometric fonctlonb:
Limits cf functions, ierivltives
cf functicns.
Deflni-tlcns :f rimits an'j examor-es. Continuity cf
functions, exam.oles. Su[t, ilfference, -r:rotuct of eontj__
nuous functicns,
?uoti=nt tf coni-inucus functicns.
Derivatives cf functi-ons. Derivatirres of sums, proiucts
an'1 quotients. Example:.
The binomial- theories-
Eor raticnal Do',rers ani exampleso
Loqafitlm: Defj-nitlon of Logarithnric frtnction, iifferent
bases, Basic propertj-es of Lorarithrns, prcble.ns invoJ_ving
these r:rocerties.
i
Differentiation.
Defi-rirtirn and exarnp]-es. conji_tion
for maxima an:i minima, Rollers theorem, Mean va1ue theorem,
Taylcrs expansion.
Inteqration:
Definitic_n of inteqraticn,
inteqral, exanDles. r*S;t;t."-i"."n."a,
EunCamental theorem of inteqral
the i-lea of rii,:mann
exampres.
.!
calculus
I
t
conti....
2
--1.
'
--/ l
-
z2.i
.)robabiiit
c- L:r- ,Lt icrt.s:
Concepts 3: :1 r ,'^:r'l-i - iL.,, f regr1o13r,., axi cr-na:ic lef iaiticns
cf :rcLahi'- i ,-
, Propcev-ties at-r1 )r')],t ciJ-ity cal-cul-ati-on.s, C,-t'.t.it-i:na1
brobability an-l a-re s' theorem.
l{ethods -rf s3n'-l': irtrl.
conceots cf po),.Lcrtir)n all samrl-e. sanr!.r': ran-1om sai-nfl1inq,
sampl:nll sy1iternatic samDfin'l
stratifiei
l'leasures of c.ntral
teniency"
l,Iean, median, Tr ^, 1-3, .f,efmetric !-nean anJ Harrnonic ncan.
Prooerti-es .)f a '-r'fol r:r.easuLe ff e--ntral Len-lencv,
It{easure.s of ''l eviaticn:
the idea .f lis--,;:rsion, ri 1le u
quartlLe Levi ation, variance anL staniari'r.eviat-i'rr-rs,
coef f icients r E .1isn. 'rsion ani variaticn.
!
Computers:
General introducti-on tc concuters; ccmponents, character
10 Hrs.
cc -'les , E]-c't cirarts an i rper:a t in2 systern, -,onlicaticn
of database in bioloTv an-l r:.sea::ch, eJ. statistical
analysi's.
References:
1. :'r text bock .cf caiculrrs, Part f : ,Sh.anthi liar.t--fn.
2. il text book ::= c,rleulus, Part f f : Sh.rnthi- rdar-.1\,'an,
S. Slan'-l .v C:. . ''{e'v Delhi.
.rrinciple.s of Rcai inalysis: S. L. Cupta, 'disha R.ani,
-/ikas Pr:l:Iishinq rcuse.
+. ,rouniati:rns of r4a Lhenatical Stai:istics: '. l. 'rupta , -1.K.
3.
Kapoor
5.
6.
j.cal ,Stati stic s. Parinal l'ilukhcpa.,lh}ra\r/
.Ierar central ilcok -igencr.r (p) Lt'j.;
Calcutta.
Cantor, ;.R. aryi Schimmel-, ?.'" 193C. tsicphvsical
.T
Che"nis'i_.ry: Part f f & fII, lrr:eman ,! iornpan.r.
l{athem--
-u
l'\
/
:
',- t
\f
\/
.?
l'gq=
IT
_
NTr)-IJ::\4T:
r.
) LV
t
f
J
ND\/
1
10 l{rs.
Chemi-caI brn'is: fonie bon''1, covalent boni/ co )r-lin: re
coval-ent bcn-1 , m=talic bon'1 s, hv'l rcgen ].;ln^'1 r;, h'r'lr;ohilic
interacti a, ,ranLei:-[aal f]rc=s, icnizati:)n').= wat3r, PH
lC Hr-s'
rneter an'l 'neasunement ,a
FEH, Baf E rs, lltrrti,;n ,
I*.
f^
qb.gc_1l -{e-e.cti m.s-: lneni'caI esuations, oxl-lation-rerluction
r.3gljlosr cxi-rati )n n,rmber, r-rrclecular It. ca]-culatirns,
solutions, solvelt tr sol-irte/ ccnr:ntratirns in solrrtions. 1C !{rs.
S{
|lydfqS.5l!.!9-q:-,'rlkenes, r:acti:lns rf aLkenes, polvenes,
alkynes, reacti rns of aikynes, pclymers.
Hrs.
^.1chho1s_: Gen''.-. a1 Itrnular clas:ifications,
reactioosr
gxidation catcllrrtic r)r:iletirt:t r? tI.ce:r:nt of OH by
5 Hrs,
halogens.
it
:t={"-[!q.: :_.!9_q?,g1s*.i R,rac t i cn s of the c,.rb)nvI.r:r)up,
"a
ri113n :;f Li'l'd/ rercti'ln
r,,rith
..Jil/
2'1, Oxi.lation, perioiite
oxiiaticn.
l1g:9r sr.
ass
5 Hrs.
:
s l!- --.1 ?i?g I c-+-!v- -i11
0_
]q.t 9n t c ornpc un'r s_ :
occurrrnc./ sLructure
lj- rssi licati:n,
rrt,iqi
an.l functitr t>f rr,onrtsachari'l es, oiiqosacchar:iJes an-r
pol-ysacchari les.
g-a&o_}1Y$,r-':
10 Hrs.
b':s:1 cn pclaritv, structlre,
,-gli"g-.S_f_-lq: llas;ifi''.tirn
amphcterlc nr.lne'-ty lf a.rnino aci's, peptile itr"naticn.
10 Hrs.
'-:,ase'.i .ln Iu-]cti )n, bas. I .>n
Proteins:
--ias l-1 f ic.lticn
phlrsi-cal ah i cil,-rical- ti::c=r---ies, .:r.-rucr-,tl:e, r:les tf th:: 15 Hrs.
rr.reak interaction
in t-he stabilizaticn cf pr.ctein sr-rueture,
hydrolvsis cf n*:1--eins.
Clas',rr i:ication '>f Iip11s, f atty aci.ls, f at s, waxes/
I,+p+js:
qlrTcolipi'Is. Terpenes an-l
phospholj-ioi's, sphinrolioiis,
lu lrs.
steroi ls.
cont1. ..
.
:4:
'Jue]eig qc_j._.Iq: .ourlnes ani pyrimilines anC theif
structures. r,Tucleosile and nuclecti'ies, classes of RAIL
and DNA, brief aCcount of thei-r .structure ani. f,rnction. 15 Hrs.
Enzlgne.g: General aecount, Qo-€Dzlmes and. enzyme cc-factcrs,
classifleatj.on anC nomenclalure mechanism cf enzyrne action,
lnhibltlon of enzyme, regulation of enzyme, allcsterl-c
enzfrmes, rsoenzrTmes (mentfon cnl.r).
15 Hrs.
BgEeleBees
i
*..
1. Stryer, L. SloehanlsEry. 1995. rf.H. Freeman
&
Companyr New York.
1. l{atthewsr H.Q-. , Freedland, R. , },1iesf e}d, R. L . 1997 .
Biochemistly r r short ccurse. ,:,t1ey-Liss. frre.
)'
3. \leaI, -,.L. Chemlstry and bioehem{rstry: a comprehengi.ve lntro.luction. Mccraw Hill gook companv,
O
:-
:-- r,
5Paper f fI.
G::dlD,.lL MfCROBIOLT-a',
95 hrs.
Hlstory cf microbloloqy - Leeuvenhoek anC his microscotre,
bJ-ogeneiis versus abiogenesis. Germ theory ,f liseaseKoeh's postulates, .levelopment in disease prevention,
antisepsls, irnmunisaLiohr chemotherapy. The concept of 15 Hrs.
sterllzation, methods of sterflization-dry hqat, wet heat,
Preparaticn of
ralj-ation, chemical an.i' titteration,'
me,lia an.d their us€s. eq: nutrlentiagar, potato fextrcse
agar, i.{acConckey agar' Eosin-Methylene blue agar, erilliant
Green Lactcse-B1le 3roth.
Classes of microcrganism: bacterla, virus, fun,qi, l:lue15 Hrs.
green alqae, protozoa, classlficaticn lf rlacteria, morphologJ-caI characterist.ics, n,rmeric'a1 pigonomy, temperature seflsltlvity, staining lropertv, biochemj-ca1 characters,
DiV'r b.rss composition.
)
Differences between eukaryotic anl procaryotj-c cells.
The morphology an'-r ultrastructure of leacteria, f1aqelIa,
piffi,
ce]I wal-I an'l its biosvnthesis, spore, volutin,
20 {rs.
nuclear material. Growth anl reDrr-ruction in l:acteria.
FActors affecting the grorvth, require'nents for carbon,
a
nltr)qen, phostchcrous an,l sul-fur. Ilotake '>f nrrtrients
lif fusion, act.ive transplrt/ croup
'ry cell-facilitate'l
Gro'uth: Measurement of grornrth, iry rvsigill ,
translocation.
colony count, oacked cell volume, tubilcmetrv etc.
Iscl-ation of pure cultures, srr'--ai plate, streak plate
pcur plate etc. l{j-crobial metabolism, acrobic an'l anaercbic
respj.ration, glycolysis, I(reb cvc1e, Dentcse ohf'rfr.t"
1 5Hrs.
pathway.
General characteristics of viruses, cultrirvati>n of viruses,
bacteriophages,,, reprgir:ction of ).{\ & R}J1 ohages, the
L,irtlic cycle, one steD grolvth experiment, aiscrption to
Pha'teacil,
1-5 Hrs.
the hrst ce]l & penetratlcnr synthesis'oflnucle|c
A
assembly ani release cf nhage perticles.
.D:
Brief acc-: r.l)t , : -n,-t ro\i 31
i i se : se )f h r:n:1, e.
1. I
tychc:' ^, c:.r--. i .
- rlhr.-:1-r:-,11o Si
l
iOth._-ri
_S,,
a, f a bi : s , rrol_ic,
lLl f t heotai__i_,_:: r,I.ria
-in 1 arnoeb-ia,si....
i
15 hrs.
[ef_crences:
1. pelz,ilr ,
.i_. -h._tn :,
l. I i{ ;arie?/ ,.J. ). iticr:li,)_
loJqia COncr:ti:s tl.- rtriic,:tions
(f;l:) .
2. ilenson, ,. t . (lsSO).
Microbiof.:q_j-,cal_ arpl_lcations:
,i Lalrr-, lorrr ,.ian
r:I in 3eeer.il .4icrobicl >m,., r.n.
, C. Br-,,.,Tt .publish.:rs.
3. .Stanie::, a. .,
J,n?r-h iln, I.L. r,fi1eelis, i4.L.,
:)aj_nter,
.D . ?.
Grln ,f.1
' Ij-crcbl-oIc:.v. 1936.
ltacmi
:
;
t
,1.
!
.l-
€
!
J
{
t
n
r3
'd
lc
!l
,,1
I
fi
it
.t
l-1,-rn )ub]-.
tdT
L,
.'-
?ai-,.:::-
T-
t-
l 'Il/I''].O irlf'IT'-il,
,-;U*1
'lfCDO:IIOLO-l'z
t
95 Hrs.
'
C {^t
Micrclorganisms ..r' bt.'i1nryI+risl crrcl'=s - crrb:n' c'rclnitroqen c"rc1:1 , ph::,,:i-r)rfus c-'cIe ani s':Ipll'Ir !--rc1?/
svrnnictic anl
f i-xation
plant micrclcc' jnta,:acti'n'
'2
non-symbictrc'
10 Hrs.
E-rl.r o:rislning e'q" botulism a1'l
Mierob:ol11-ir ,.lF ,")^ I
Staphyloc.;ccaI?;:)l)oisoninl,]ifferentc]-.tssescffo:d
/
ter:hniques'
fcc'l preselivat'i'lr:
r,
nr=.
-Jater micr:bio]cgy:
sfur:c -s cf microorganisms in ]^/ater.
:vater pcllut'irn:
wtrter
E. coli as the in^-rrcatcr lf
rn dn-l
purificaticn methc'"s se'r'1m-ntatilnt filterati
i:];tcl-ericl:tr1cai ex:minatj-on of w;lter-ore grrmchlorination.
a.o
* hrs.
otirre testr C>nfirri"l test and ccmpletel t':st'
Municipal waste anl in lustrial ef f luent treatnent '-rn'l
lnt
4ispcsal, -n :':'re'-ricai treatin"nt' biologi-cal treatn
filter:s' ::ot-itin-'r
actj-vate olu'l?e proCessr biclolical
anaerobic
biclcgica' C.nLraCtors, f luidj.sel bel react,.>rs,
Qjnl;).Ct dil=sters, oacke'l' cll''mn r-actDrs'
treatment
effluenttre,]t.nel)tinTannerwan^]ouiplnfrrstrv.25hrs.
n,
I
I
I
I
i
I
Degrai.ltiln of pesticire anr ;tircr toxic chenicals brr
micro crganisms, lriotechnclrqical a'or:Iic 'ti'rn lf thurinq20 hrrs.
ensis toxin as I natural cestici'1 e'
..Iaste iispcsal
i/errnitechnol'>qv, rol-': of eartll rcl:'ns j-n
ani biomaqnificrtion ->'r nr-lt'i3rlts'
Ref erenc.:s
:
!
l.
Jog4an r, ;. i" 1995. Envi'lrnrnental Biot=chnclcqy'
i{imaIaYa Pu,'-ishinq -{:use, 3ofi}cdv'
2.
l\4oo-'a3unq/ 1'l- L)96.
i-*--
3.
-
Envircnmental'3ift=chnolol'r'
-(iu'ver,
fnc'
r)rinciol,:s .,rr.. I "poiic':t-ilns,
"{cGraw-FlilI
1?93'
Pelezar, l'i. Jn' ;lhan' l':' l' & I(rleqr N"']"
}.{lcrc.bioIlc,Crnce'tsan]1ooIic..ti;ns,llcGra*.HlIIrnc.
5 hrs.
(
'{;f-:
.\
Paper V.
CELL Rf aLO l''/ t GE\-lTf aS
95 hrs.
CeIl tsiologY
Cell- as
a.
basic unit lf Ii-rinq srrsr-ems" The cell theorz 5 hrs.
PrecelLular evclution
lrcai cLassification 3f cell- ty)es: PPLOs, bacteria,
micrclles, plant- ani .lnlmal- celIs. ,i ietai..l.er
",rio3pr7ctj-c
el-assificati:n cf cel-1 tvpes "rrithin an rrqanlsm' Ce]1'
.)
tissue, orqan an.l orqanism as ri i ier:ent- Iev-'1s i lrgani5 hrs.
zation ff :tlrerwise qeneticall\/ simil.rr celf s.
itr-r-:.cture rn r function '.->f olas'na 'ne'nlcrane, ceIl :rganelI':s'
cwtf solr golqi b:-lies, =n-loolasmic reticulum (rou.Th anr,
smcoti.r), ribotsorTlesr CytoSk' letal strr.Ictur-s (actln, micrdtUbul_esr lntermeiiate filament), l4itochoniria, Chloroplast.s, f,ysosgmes, persxysrlrlQsr nucleuS (nuclear membrane,
15 h,rsr
nucLecplasm, nucleolus chrcmatin)'
CelI-iivisicn:mitosis&meosis.Briefcvervier"rcfceLl
cr7ci"
CelI lo"o*otion (amoebcil, flaq:Ilar an'r ciliar'')
gerelisq-rlature ,of qenetic.i materialr nucleie aci's;
5 hrs.
Dr\-l repliCaticn
Men',elian laws ;E inherita.nce, l2ne inter'ctilns
5 hrs'
Sex -leterminatj-:n in clants :nl .:ni"naIs, sex-'linkaqe, n1n(as a pro6f lf ch'lm-rsrnal- t- '3rpr7 ->f inheritance)
.risju:'rctj-on
Linkage, maooinq qeneS, interferenc--,
coincilence ln pro-
anA. eukar',rctes.
5 hrs.
crgapisatj :>n
cf chrrmatiCs, eentromercs, tellmeres, chr:matin' nr'tcl-ers'Te
crganisaticn, eu.ani hetercchrcmatin, s,r:ecial chrcmcscrn:s
patt':l'ls
(=g. , oclytene anl Iamdrush ehr:mrs:mes) , ban'l1nq
A
10 Hrs.
in human chromcsomes'
Chromcscmes: Chemical ccmDcsiti:n, structural
:---,
^I
\,
:9:
Structural. anl numerical aberratj-lns iir-rllrrlncT chrotno-'
somes,' evolution of 'vheat' cctt2n anil rice' Herciit'-l:"
defects - i(l-einefelter, T\Lrner' Crj--iu-Chat an-l D:wn
10 ;)rs'
synlrcmes.
Mutattcns - splntanerus an'1 inluc,:d, chemical anl phvs:ca]
muta.lens, j-n{r.:'ce-l mutaticns in plants' eninals an-l
5 hrs'
. micr>bes fcr eccncmic benefit :f 'nan'
Basj-c microbial geneti-c,sr ccnjugati-cn, transiuction,
transf:rmation, isolatlln tF- auxctrophs/ replica platinq
techni-rues, analysis )f mut;iti)ns in hicch::''nlca1
. p.-lthwal/s, cne gene - ln" =nzyme hyOcthesis'
Extrachr-cmcscrnal- inherit
r.lc.r/ mit-:chonlrial
an'l chloro5
pla.sL g=netie svstems
P
@bcr:ulation qenetics:'1ariyan1 qenotvPic frequenci'-s
5 l rs'
l,DeRoberti-srf.D-P.!DeRol-'er-tis,l'l''l'-'1995'
CeIl & l4oleculer gi':I')'r\rt R'I' ''Saverfv, i'Iew Del]ri'
2.
Irclish, H.., rl.:.ltimore, D. --lerk, :'! lip'rsky,
ggS) , IIclecu-l-ar
S. L. i'4atsu:l.aira Perrl & Darnell, ,I. ( f
€eIl- Bj-o1ogy, Scientif ic "merican '31:ks'
3.
Fri-efeIler, D. 1990 )'tj-cro'!:iaf ienetics, ''Iar)se
Publishing Hcuse, rrTew Delhi'
4.
Russel , P.J . ,
Lcnqrnan Inc'
f sqg)
.
-r; 's'
Weinberg equilj-briu_mr qene
aef.w-:
(
20 hrs.
Genet-
j-cs- 'rrd:lision lllst:i -''-
t
,
-,
\.1
tt
.
Paper ,n
-ll
1n
.
av
I''IOLECUL;|R Bf OLOGY ^'trD
DNO T'lcHldoLo'l'1
I
rlOI'{3I'J',1TT
?5 hrs.
Mclecullr basis )f Iifc
Str,.rcture if
1 Hr.
o4 hrs
Dr.T\
Dl{,i replication in prokaryot}s 'ln-'l euk.:rrrot.:s
stmcture in prckary"t.?s;
Transeripti:n in Prckarvotes opdr)nsl;'c
Cene
-
5 hrs.
Tr-ats€.***er+h-.-'
Gene structure j-n eukaryctes'
t
3ii_ef acccunt :f transcrj_otl:n
anl RlJ,i processing
5 hrs.
Genetic Coie: )ecipheriruT th: q::fletic c'rie: Eeauul:e's
the gepetlc co ie
10 hrs.
:>f
.10
hrs.
10 hrs.
Prctein synthesis in crckaryltes an-r eukarlr:tcs
r=sgqreT.:
- -basic steps in q3nr:-clrnin':
( outlin:)
fntrc^ueti-on - Defi-nititn
Basic tocls tf tne t:chno1c7 : D.estrictiln
Iig-ases, mc'li-fying enzarm?s
:nr-'n'-rclersls/
10 hrs'
elcning v:ct:rs: Baslc 'lesiqn 'lll cl,lninr vectlrs f lr i:'se
in pr:kary)tic anl suka.ryltic svst3rn - olasmi-'l v:ctor,
bacter.icphagc- ]:,isel v?ct)rs, clsrni-ls, phaq."mi-rs
dl moleculcs i-nt;
an )vervi':'nr
IncroducJ-ng recorn]:inant
clo^l
animal cells-
D
lr
15 hrs'
lcteri -ir clant
10 hr's'
Sel,tction ani screenlnX )-- r:eccmbinant clcn:s-DI-l >verview 10 hrs.
.-ipolicaticn rE Rec ) i i tecnncllry-an
,v=rview
5 hrs.
l/t
\-
l:
- 12.r-r
ii
.
11
3gf_.:Sg"-_".9'
Treifelier,
1.
D. & Malacinski, G.'n-. :1 lcl (or latest
elitl:n) '
Essentials cf ucB-cutar Biclf,lrv. Silc:nr' Elitlx
rones
Prrblishers, fnc' Lf,n-'l3n'
1997. Oxfcr-i llniv. Press,
Leruj_Dr B. Genes -Ir.
Oxfortr Neur York, Tokvo.
stent, G.S. 6nl calenler, R. Mclecular Genetics 1')35'
i'
Narrative, IBS Puhi[ :]-: r'j'+t'{
.-,ifr rntrcluctive
Distributcrsr I:Jew De1hi'
Bartlett
2,
3,
i
4. t4,--lty, S.R. Gcnan, 'T.f ., & Treifeller:, D' 1994'
Micrcbial Glerneticsr Secrnl E4iti:nr Jonas anl Bartlett
Publishersr rcston'
5.
6.
';[aBEcIl, J.D., Gitman, M', ''rlitkowskiz'T' a-n-] Ecl-ler' M'
L992. ReCcmbinant D;ll-.. seclnl Ir,1ti:)n, scientif ic
' Ameriean Bcoks, W.H. rre')man anl Cc', New Ycrk'
R.'{. an:l Prinrcs:, s.B. 7994' Principles cf
qene manipulatj--)n -i\n introducti)n to ccqn*'ic
glackweIl'sciantific
Genetic Englneering. 5th E-lition,
PubLicatl:n'
oli,
ns/Lz/a/
&
l
l.
\
*
4
:I:
Paper vrr- lMMuirolocy &
A,':rrprAL
bell
13-
troTEcHrJol,bcy
95 hrs
fntroducticn to rnnune system/ types cf immunity, j-nnace,
acqr-irec , pissive v tc a.ctive " Antigens : types, haptens,
immunoc;}obullns -siructure and function
i
5 hrs
A--rtigen - antibcc.y reaction. affj-nity, avidity, crcss reactivity,
scecificity anc types & application of antigren antibody
15 hrs
reacti-on
Ti:e lymphoic organs : Primary lymplroic organs anc
seccncary tymph<cic. organs, ce1ls of the immune system,
cel-ls, nuII cells, mc,nccytes anc Polymorphs
B&T
15 hrr
response, Humc,ral .end cel-1 mediatei immunity, clonal
selection tneorY ?jlc memory. Generation of antibody
'r ivcrsi-ty.
10 hrs:
i11im,-;-;e
,
Aut<-irnniulity a:lr- :utoimrrrr.iile diseases, e .9. thyroc.;iLis ,
perlticious a-na.eni.., S--,E, Ct cdpasture's syncrome '
5 hrs
i-lyperse,isitivitl' reactic,,-ls, types of the hypersensitivi'-ir
reactlons, introc,uct-j----l't 1-C tunor -immunology, immune
respcnsr tc, tumc:s/ iri,.T' cell- response to tumors, turiior
specific antigens, j-r.,rl.runc ther.-rpy of cancer, ceveIop15 hr::
ment c;,f v.lccines "
AnimaI cell cultu::'c :
introductj-on
History of oevelc.rpment cf cell cultures,materi-als.orcrans.,
10 hr
-f
to basic principies of biclogy of c6Ils in culture,- cnolce/-tissues : Prj-marY anc'
,
r II
Importance cf growth factors of
establisiiec, cel-l- Iines
ht
the se rum cell- fusicn, t ybricoma technolcgy anc monec]o- 20
nal t',b ..odrl6t-icn, .rp:lication .-rf animal ceII cufture
for stuci es on gene expression/ procuction of vaccines in
q'rovrth ilrcmcting factors on proliferation
a-nimal- celIs;
of animal cells (eg, IL-I t IL-2, erythropoietin etc.)
t:::::::::_:
1.
Drc'st-ff , J t' I'Ial-e t
Immunology / Mos:y Publ-ication '
Roitt t
I
D' L996'
New York '
2..Tanis,K.L)9?''Imnu1.,]--gY,Freeman-'.}"]re:wYork"
\
\
\
lL
. t.
References
1.
fcLtn
:
,r" ,lccrqe (fg9:).
F-Iant Propagration by
Tissue Cu: :urc,
2.
Ixeqt-i. j :s
l_tc
Ecrvin, F.
.
fhe Technology
Georqez 1993/ 1996 . plant
Prop._igation by Tissue Cijlture,
part rf
practice If Ecl.
pier-ikr R.t.M. 1989.
higher p]-nts. Martinus
Dorirecht, Netherfands.
4.
Bhajmani & Razdan"
Theory and practice.
tr
Reinert
Narayenaswamy
Cul_ture, Tata
Ltc. r ltew
Def
,
L994 |
Mcgraw
hi
fR
In vitro cu]_ture of
trti jhof f publishers,
Plant
& Bajaj,
anc Organ'Culture,
S.
rr
.
3.
a.
-
Tissue
Cu_Lture,
Ec.
,.o-'".
ll- F
Fractical
Faper I - tjIOCIjEI,IIC: L
,.,0,D
IiICRC-IC,LGGICz.L TIC'il:fQLl-s
Bioc_hemis_t5y,
Freparatj-on of ouf fers: e.g.phosphateouf fe::, citrate ouf 1er, Elycine
HCI buffer, Trj-s buffer etc.
Estimation of suqar j-n Eiven sclutii.rns anc. -In oi-,Iogica1 sarr;,les.
Colurii,,etry anc I,aI-er cjrr.rrcratcgraphy
Extract.icn anC separati':'n cf Iipii's by tlrir: laycr cltr-rrratrqraphlz
Separati. n ani e*stiii.atii-n of proteirrs by c:Iori i.etry
IsolaLJ-on & Estirr.ati.on cf D!1A/RNA Dy coli.,,rirr,etry
!+cro!+lggI:
r'septic techniques:
Cfeanirrg cf glassware
rreparaticn c-,f mecria, cotton plugging and sterilj-zation
Eers rral hyglene - l.,icrcoes fru,r, hancs, tcoth-gur:, doc cther body parts
Is.-Iati. n - f microorganisr,,s fr ' aj.r, rnrater anc soil sar:rplrsDiluti';n anC pL,ur F,Ia ti-r'q.
Co1..rny purif icati:n
Dnurr:erati.-n of m.icroirganis;,r-<. TctaI vs.ViabIe ccunts
Gram staining, i,,ther stainj.no
Id,entificatii..,n -f isolatec bacteria,
rnethcos, metaoclic characterizaticn (e.g.Tl'.l/i C test)
Gr.:.:wtl-t curve -rf nuicrc't rganisms
Antibj-cl-rc sen :-tivity
of microb s, use f antioiotic
c .rs --s
Srsg-.-=
, D.-L'.1988. trn f ntruductili-r to i:ractical ii,-chenristrlr
Tata i'iccraw HiIl Cc., liew Delhi.
n Lar-oratc,ry
2. Eenson, t-I. J. 1990. Mrcrc,biological applicati-n.
i',,anual io General t'iicrcbil;Iogy, 5th ec.'i.li.C.rrwwh Fubf isieers.
3. Cappuccin,;, J.G . anc' Sherir,an, rl . 1996. li j-cr-bi.-.1:,gy. A Labordtcry
iianual ,:en j artiin/Cur'ulrj-ngs i ul-lishi-r',s, Co. Inc.
1!. Fep;er, L.L. , C.E.Gerr:ra anL J.J.irendeCl'ie. 1995, Envir-nliental
jr Lao;rat-ry ,',anr.ral-, r-,Cocenlic iress, Inc.
liicrubic,lagyl
1.
Pl-ummer
\^
--
.tr_l:
tv
tg
rl
h
il
E'ract,icel Iap-r If I.
.
2
t:El,,{C],S
Ii,i CEI,LUL
.
I,
I'lCLECUL.e'R
9y-t'.]:Slg a_1. p--r-elga_rg..1fi:!-s-:
l+ Fixati,_,n, Cehyc rat ic;n anL staj-ninc' .rf tissues
o. Squash in stain
c. Embedcling anL sectj_uninc. cf tissues
9gll_"_"Sl_t-r:I_9- t,:e.r1r_-gf
:
ar The heeirraeytvmeter ancl cther
3
..'.
lf_a_Fg_r-eg1e{r_t_s;u-i.!=h_ -*S_.r,__e_t.p_.
j,l*
aj_cs
*1"r9gr_!_1rpc_r:i-sSgfi-:
A. Cali,prati.i'r cf LCC _1ar mrcr,rnreter
I). Fin<-rj_ng out dveraEe cell size
c. Chr;:mc,scnle Iengths
t"iolecular oivl.oav
-+-
.
is\JIdti-n fr-1, suiLable rr,aterials
plant material.
2. DNA estiiir.tj-cn ( c<.-l-rirnetry methci )
1
DNA
(
e. g . E. c;:.11/rat
3. rncuctiun r:f Lac cpercn usinq rnicr,--rceni-srn
4. Ccnjue€ti.u'h in E.ccJi.
river/
e. q. E. c,-,Li
3*_fSJSr_cSS
1.
;.Ilyr: Brecman. 1996. Lac i-t ry i.vrstiaatj_r,6s
ltoLecular Bi.-lcgy.
J,-]-r, ,,ifey & S s.
2,
Flummer, D.'1.1988. no Intr-cu:ti-n
'I'at.a }lcGraw HiLl Ce ., i.iew De1hi.
t-- f ractical
in CeII
and
Biochemistry-
\
-
f? -*
Fractical ia
1. 'iiist:1cgy
r fII
Ii,i1ui:CL(-GIC.L . ]-T'HCDS -.]tD CULrL--rl: .j'ECi. if/,;UES
,,f Iyn.pnr.id
i.fr_ eos
2. Elr.,c;c Ar-upinE
3- irre'-erntiun ,-f antigens pri-t.c,-l :.1 ii,,n'untzati. n ir,. rabb1ts,
ttiethcc.s uf oleeci-nE, prr-( ucti -n ,.f p-.rycf -nar antibc.c,.j_es.
4. 5eparati,.,n J Iym;h. cytes f r.. rn o1,,._ il
5. r.ntiqen - r.lrtic_cy reacti ls _ Haeri:accl_utii:ati.n
6. l,' rrparati-n - f rnec.ia, Iy,, ph .cyte cuf tiv.ti.- n
l. Denrc.,nstroti,.n -f rac_.ia1 irirnun..crfjusi_it
8. Separati, it , f ir;'nrun,- g1 _buliirs oy precipltal_i - r anil electri.ph-resis (c,ern,-nstrati -n -':,Iy)
9. Lem nstrct-i-.n f lrli-ea
I0. T;enr.,nstr.-ti-. t-, f -cec -,.tcary f. lLicles in spleer: ."f rriice
1l . Cl ninE ,.,f anir,al cel-l-s -y cell- ancl c1c.n1, purii icatir n.
12. i:eC iurLi pre !-crr<jti ,i,s, surf ace steril-J-zo't r F, in-,cul-aLi- ns - f
pJ--n t rr,aterials.
b.
. i jecir-:lt, i re l.aratj -,,ns
a. St-.,c',< I reparati-,ns
i ) i acr,- anc llj-cr:- r,utrlcnts
I
) Li frli., n; S
l-f l- ) v l-LdlTit-:; s
j- j_
I.l. ijil uCir=tnrents
c. !terillzatir
a
1) Cutt. h p1rag9-inE
ii) -ut,cIavinE
iij- ) ixplant c: 1l-ecti.. ns
iv) S t-rrf ace s-.t-ril-izati'-n
v) [ ractic'.:s in Lamiie, f 1--'"v c]-ratnocr
vi) . erS- ir:El- ilyceni-c
d. In- cu1.:ti - lrs
i ) I'1,- nit -.rj.nc f-r callus i-nc,ucti ,.i di'
lt_i
G
:ie('e.l-rf,
ti,ilS
f-..renc-es:
1. :i€r lcrni, f' & .-:Vic , L.'L. (Ec s ) .79g-y..-ut io,,clies:, ,+ Lab-rat.-ry
i anuGl-. C- l-t :jl--ring Haro,-r Lao-.rcrt-ry
,'r.il.,,droul-ies, D-ll .Shevacl1, E-r',.an( 1:.
2. C lioan , J.E.Iirui-s-eci.,
itr,-ogr
7996. Curr,.nt I r.,t t:- l-s in Itnrt.t.rr.t-,I e11 , .)-it,-: r,iley & 5.,ns fnc3. Liir t-i, it.ii. i. C,-i;'Lales, R.- (Ecs) fg:C. iIanL Ce11 Cu1tirre - l.
,.Fi-r.r dC.,, IRL I res'-, Cxf .rct.
I ractical
.t
-.'issue
'I'echliiQues
an( rxperirnents
Culture
4,. Snitn, .. i.I9c,2 . i'Iant
i-codelnic i ress.
- --4: -a.-- ------^\
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sa
"B.Sc BIOTECHNOLOGY
There
will be eight
theory and three practical papers for the examination. The
Order of papers, duration of examination and marks allotted are as follows:
Name of the theorv Daoer
Paper 1 General
Year
Duration
External
Internal
Marks
Frst
3 hours
80
20
100
Second
Second
3 hours
3 hours
80
100
80
20
20
100
Second
3 hours
80
20
100
Third
3 hours
80
20
100
Third
3 hours
80
20
100
Third
3 hours
80
20
100
Thtud
3 hours
80
20
i00
Microbiolow
Paper 2 Biochemistrv
Paper 3 Biophysics,
Biostatistics, and computer
Paper 4 Environmental
Biotechnolow
Paper 5 Cell Biology and
Genetics
Paper 5 Molecular Biology
and recombinant DNA
Technology
Paper 7 irnmimoiogy an<i
animal Ceil Technqlqgit
Paper 8 Piant tissue culture
and Biotechnolosy
Total of
Name of the Practicals
Paper 1 Biochemical and
Micro Biological Techniques
Paper 2 Methods in Cellular
and Molecular Biolosy
Paper 3 Immunological
Methods and Culture
Techniques
Report based on educational
800
Year
Duration
External
Internal
Marks
Second
i 2 hours
80
20
100
Third
12 hours
80
20
100
Third
12 hours
80
20
100
15
15
60
60
Comprehensive Viva along with Practicals 25
Total for Practical
Total for Main (Theow and Practical)
25
visit
Laboratory Records (to be
equally distributed x 20
marks each fro the above 3
DaDers
400
1200
'
rt
-4' t
+
Attendance
:
4 Marks
2 Test marks
.
5
Seminar
:
Assignment
:
X2:
l0 Marks
'1X2:2
Marks
2X2:4
Marks
:20 Marks
Total
Marks awarded for Intemal Assessment will be displayed on the Notice
Board. Grievance redressal
Els
per existing university R,Ies.
B.Sc Bioprocess Technology (Subsidiary)
Name of
Papers
First Year
Paper I Bio
External
fnternal
Hours
Total
50
l0
3
60
50
10
J
60
50
l0
12
60
20
Process
Technoloev I
Second Year
Paper II tsio
Process
Technology
Practicals
II
2oll
Totai
Practicals
20a
will be spread over two days in order to accommodate over night
incubation observ'e, the results and to conclude the experiments on the
second day.
Distribution of Internal Marks
Attendance
2 'Iest Ivlarks
Seminar
Assignment
Total
'.
.
.
'.
:
2 Marks
4 Marks
I
X2:2
Marks
2X1:2Marks
l0 Marks
The rules that govem for the Chemistry subsidiary shall be followed as in
the case of Bioprocess technology (subsi{rary).
Fly UP