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Document 1732001
UI'TiVERSITY OF CALICUT
?
* E'sc--Eio Chemi'stry (mai,r ar:d
(fibsirai:t'
str'csi'Ciary) ccr.irs,=.e
from 2005-06 admission c,[\r/,31.d:j - apcrcVsC
G ENr
-
EI+:.L l,l,i D _A,l.i,F
E
Ftl.i\lc l-: |,.i'
ill irl
"3
Nlo. GA.. ll J 1 I 44 I 3i2001
;;;;-,
- i,ri,'.iauci,cr of intt;mal assessment -with effect
i:l:- : j.
.:]r,:ltrS
Daia
; ilil;;il;il;;;;'il;L";t;,;,,
2
]!.t\o
18.05.2005.
l,
C.aiic._r
S EC-i-l
t
tJ
ON
n,ver,sit)r. p.O., 20.07.200s
;;;,,,;;,;il;,;' ;; ;; ;; ;;
l(,l3) oftheminutesof ,t',eeti,gcf Facuii.rof
sc,;nceherdon
,
I
3. ltem No ll A (28) of :he minutes ol rne:ilng ci, A;acenrir; cior.rr.:cil
held on
18.6.2005.
The meeting of Board of Studies ,n%ffif,",*,n, held on fi.04.2005,
vide item no. 3,
resolved to introduce internal assessment to boih B.S; Eio cheinistry
(main) and subsidiary
courses for theory and practical papers as foliows.
a)
B.Sc Bio chemistrunain :
10 (ten) marks each for the eight theory Fapiis !: set ai:art a:l
iniernal marks for B.Sc Bio
chemistry (main)
The split up is as follows
Paper I to lV - External
lnternal
Paper V to
Vlll-
:
Extemal
lnternai
- 55 rnarks each
- 10 maii<s each
- 50 rna.r.s each
- iC m;r;is
b) For B.Sc Bio Chemistry (rnain) practicais
practicals shall be the intemal mar:<s.
-
ear;h
2C rcerks
or_it
of 100 marks for each of the two
") 10 (ten) marks each out of G0 marksEch
ior t!:eon1 paper I anc ll end pracii&l paper
shall be internal mari(s.
2)
3)
4)
I
The mee'iing of Facult'.r oi.icience ield or: 14.05.2005, \.,ide itern no. l(13)
appro'red the minutes cf Eoarc o,i.Stuc!e.s.
The nreetlng of A.cecercic Councii lreid on 18.C6.200S vide itern ll A (2g),
appro'red the minutes oi Board of Studies as approvec.t by the Faculty of
Science and aiso the minutes of Fa< ulty of Science.
Sanctlan is therefore accorded fcr imJending the revised scheme of e:<am of
B.Sc Bio chemistry (rnair; an,i i. -jb. wiih effect from 2005 admission as
suggested in para 1. (a c. c ) abcv=
Orders are issuetl ac,toid.n{lly.
"
sd/.
DEPtr']- r' REGISTRAR (G&A r)
FAT REGISTRAP.
fo
Principals of Coileces cfl'ei:ni: 3.S: Ei.:. Cternrstrs, lrain & Subsidiary)
- toThe
copy
. cE/ University lnfcrmaticn cent:-es i AR, D,i /f sci; si/FC
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Physieel_ j,si;:cts
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Bio-r^,:-r:,c,r;ic Chliln_istry
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General- :ic-Chernistr
of d-:::
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Clinica.t- & -inal.rt-icai
Biochemi qt-r'./
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(Theor-I & ?r_.ctical_)
Total. f or: 2 s,.rbsi ir.-,r-,
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PFr\asroloc'r/ x(r.lRo'lroLa':,': t ir rlrrr )'d 'rR .BroTECIfJor,ccy.
subject
The svIlab,rs of r:iDer i- (treeory) : pi-vsical .sp:cts;tnl UethoCs
in giochemistr:y uras a't'trr:v:ri i:y the nc.rr-: or- .Sl-,rr1j,r:s, lacr.rlt-., Of
l- Science an I thc -.ca lr-Ti.,: '- :r.rnciI.
-'t
R. ;c. (srocHttl-t ir- .' .i'I'.rN)
TI{EORY.
P,iPER
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BfO - O?G-'NIC
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1. Carhohydrates
Basj-c ideas about tl-re loiocheraical functions of carl:ch=zjrat:r,s.
Classi_fication of 6-:pbohy<1r,rtes. Cl_assj_fication of isomerj_sm 'r'ith
optica-l- activity- definiticn,
exarnrcles o:Ec eah.h sub civision..
determination and apolic.rtions.
MaQarotation anC its explc-a.nation by
the rinq sr-ructures o,f ribose, glucose, mannose, qalactcse .and frucr-ose.
Structure of the foll-oraring sugalr Cerivativ.es: meth.z f-alDha-D.qlucopyranoside, 2-de:x--., - :eta-D-ri-1:ofuranose, Qluconic F.cid, glucarlc
acid, glucuronic acid, ::l-.,rc:ro1 and scri:ita1.
Structures of the Foil-o-.rinq Cisaccharides (Ha'vcrth ncclels) Maltose
(:l-ucidation of the
isomaltoqe, sucrose, l,.,rcto.se anC cellobiose.
structure cf nono, di rr':<1 polysaccharide.s is,not included) r
ir
Representation of :he -:.r-'rctural f{=,3tur.js of the follorainrr
polysacchariCes,' lrnvl3s ,. ,-:rn.,'Io pectin, ol\,.cogen, cellrrl-o.se, cl-litin,
heoari-n, an.1 ::eci--irr ':l-irci.1.---icn of the strrrcture of Dolysacch=,rides is
not i-ncI,rded) . lhemical- r-:.'ct1:ns of qlrlcose, frr.lcto,se, maltose.
Iaetose, suc.ir,3g,;, sts-arch and gi-yeogen.
2. Lipids
Definition
of l-j-pio,s. -;1c il.:ls alvlrrt the bioch=nic.-.1 functj-ons of
l-ipids. Cias-sii.i:.ttion i':rl nomenclature of fatty acids. ,structures
of steqric aci.l. p,:i;ii,.:-: . .:id., oleic acid, ]inol_eic acid,
linoleritc acid (:l-rr- and garnma) anC ar,-tc:iclonic acirl. Structure of
.laDonifjc:,-icn and ernulsi-'icatj-on. Definition of
triglycerirles,
saponif ication 61qmbet, ac:d, .numberiand loCine lnpinber of -f act:
.,
classifieatlon of phospholinj-.1s. - Structure ofphosphatidic acj-d,
Lecithin, cephalin, phoB-)1,11. ify f serine, plasmal-ogens, sphin.Tcsine,
eeramj-rle, sphintoriy:Iin -nC -r.,rlactocerbrosj lr,-. f soprenoids-exemples
of monoter?enes,chi:micaI structure of carnphor, rnenthcl anl qerlniol.
Chemical strrrcturr,s of th: foll-owinq st:roids : Chol:sterol,
. :
cortrs'li, aldosterdre,
ergosterol, chole chole caiciferol,
testosterone, estradiol, sticXnasterol. R:lactions of sterols.
Definltion of saoonirication numbr:r, aci-d nuRber and iodine number
of f.:ts. Chemical structur,l of cholesterol and eroqosterol
(without proof) Col-'our rc.rctions of st,:rols.
Chemj-strr of natural
rubber.
2
cont.l
t
f:
t.
:
3"
r
i
I
I1
\----
":---3.
Classi.fieation of amino aclds, name.s and structures of t-he
2C protein
amino acids chemieal rea'ctlons of ami_no aclds. Represen:,rtion
of
Alanine in the zr+j.tter ion, anionic and cationic.forms.
Basic ideas
about the classificatj-on and functlons of proteins. pepti,c:s;
forrnatlon of peptiae bbnds. .Structures of glutathlonez oxytDein
and
insutrin' Elementary study of the prlmarv, secondarr, r,ertiai; and
qua ternary structures of prot--ins. Basis prineiples of
the methods
of proteolysis, and sequencinq of proteins (sangers and Edmans
methods
only) . Denaturatl:on cf proteinsr precipitation reacglsn.
and col_or
reactlons of proteins.
,
4.
structures of ptirines and purlmidlnes: adenine, guanin:,
cytoslne, uraciL and'thymine, structure of .edenosine, 2 Dcoxv
chrernleal
adenoslne, ,\MPr
ADP,.1?P
anrl CyCLfC lMp,
Classiflcation of mrrclic aci_cls : primary anC sccondary structur
_- of
RN"r and DN\. (physlological rore of nucreic
acids will b: r_artght
only .Ln the third year.)
5. Secondary Metabolltes
Brlef account of the distributlon and i-mportance of seccndary
metabolities ; phenolic compounds, alkaloids, terpendj.ds and
fl-avonoids with exarnpres. (chemical struetures are excruded)
.
:
.
a
I
&
5
PAFER TTT
..'-)}IY.S
TOLOGTCAL
A,:p:cTs olr
Broc'.lEI./fsTR1,a l.l,rn
rrTr-n?
TTTar.,.
. _ -__n and
absorptlon
and atrenrn#{
.1. Diqestion
Digestion and absorption of earbohydrates, proteins,
and r_i-roids.
Digestive
enzymes amd their action. steps ln trre
actj_vation of
proenzymes in the
tract.
ceneration of gastric r-ICr. Composition
-q'i'
and functions of bi1e, enterohepatic reciruration
of ]..ir-e _ salts,
the structure of villi ancl the portal vein. Defects
in the
.dicrestion and absori:,tj-on of carbohydrates .
.
2.
Biochemistrv of b]ood
constitutents of hr-ood-t1"1"" of ce,ls,
comtr)onents of plasrna.
'Types of plasma proteins
and their functions. Technism
coqulation' The coagulation factors, antj. coagulants, of br-ood
fibrinolysj_s
Structure and functions of hemcglob-in. Brief study
of abnormal
herocrlo'.''-ins:. .Siel<Ie ceI1 anem-ia, thalassemiag,
HBM. Formation and
fate of
]-.il_e piqments.
iti_on
Transnort cf o2 and co2 in bl-ood, carbonic anhydrase-the
chloride
shift. The structure of nephron, renal excretory
meehanisms,
nature and composition of urine. Tests of renal_
function.
Rer-ul-ation of water and electrolvte rnetahoij_-sm,
huffers jn blood,
respiratory and renal regulation of the i.!l
of bl_ood.
4. Fig-gllem:isr!ry of reproducrion
Chromosomal dterri,inat.ion rf sex, The hur
an reproductive system.
Hormonal regulation of testic,r-ar and ovarian
function
Puberty,
pregnancy, and lactation_Biochemistry of
mif]:_coLostrum.
5. ' Biochemj_stry of Nutritjon
Major human nutritional re.Tuirements. Calor-ific
value_Bjological
value of proteins, effects of protein and calorie
deficiency.
Essential alrd non-essentj.al amino acids, essentia-l_
fatty .rcids.
vitami-ns-names, comrnon socurces, maj6r r:iochemicar
roles and
effects of dietary deficiency. Dietary tr__be.
E_rqnemical role of
trace elements.
sues
r
different proteins in
of energy for rnuscular
n of nervous tiisue, .""tr""i"m
or
]e of Ca,p, Vltamin D ancl
Composj.tion and orqanisation of
rlss biologlcal memblanes
sou?:ces
BM/t4/e/
\
r-
P^PER TV
l'r\ITEi1: EDT^R\.'
i
l-, --L-i
f
1 . _[g!Eg]g
-of study or_. -SlgPg]irm
Outfines of the use of la}--oratory anir:ra1s, tissue
slicer;, rnicro
or/ a,"isms and jsotopes jn the stud.., of metahofism. ltltra structure of
the cell-prokaryotic and eukaryotic. riub cel fuiar fractioraticn.
properties cf the major subcell-ular ir€rrtic-'l.es.
2. Enzyri:o1oqy
Elementary studrr of isol-atjon of en"ymes rnd the criterla of purity.
T.TI.'1 . System of classifj-ca.tion and nomencl-ature of enzymes wj-th one
example for each cfass. Ivlethods of measu-ter;,€rrt and expression ef
enzyme actj -,ity.
PrincipJ-e of nrethods of _isof ar-:on of enz\rmes.
t)lementary study of the fol-lor,vjnq factors afj-ectinii the velocity of
enzyme C,:ttalrrsed react-iOn:-. E-!f .1it't of cr, zvme concentrat-ion, temperatUle
PH enr--' sr,-1:str:at.e conentration, The M-ichaelis lrenten equ-tion
(without der j-vati.)n) ffre Lirie '.. sver Burk p1ot. . Definition of IZm
and \rma" values alrd t;reir detr-.rmjnatisn by the DouL--Ie rec--lprocal plot
neti.:d..
Definition of enzyme spec-ificity, l.n examj:1c caci. ror substrate
.:pecifi<':iLy,, stereospecjf icit',, ai-rd cDfact,)r slre'-:l..i 'itv of enzymes
from the pathways prescribeC f.-l s'i--'--,. _iu '-l'ie cour-se.
Explanation of competiti-re an,-: t 1n ctmpetiti',ze tlrlles of e:rz)rme
r-he ]:-s:is o: the dou]-1c reciprocal
inhibition and their distinction:n
t*ran
plot,
Brief study of al-fosteric inrik-,itio,',
an examl e.
METTTPOT,TSM
Brief study of activation of z',zmogen forms cf enzymes. .,:tivation
of S'-t enzymes. activation of enzymes by pi'ostlretlc crroup:i, coenz\rmes
and metal- activators.
erj-ef study of allcsteric activati';,n wi-th an
example. Chemical structures of the fol]crvinq coenzymes.
contd. .
i:
t
._)
I
-1
+r-
NraD', N'DP', ri,i'r, F\D, TPp/ plp/ lipoic
aoid ancl b.ilti;.
Bia.chemical- reactions where these coenzymes
partiripate
exampJ-e each' ('mechanism of a-ction
is not expected).
3.
I na e lgbi c
J_e_F hgl
wlrl_r an
i sI. q f_-ca lboh$l.it_e r
Ir4er,zerrrof - Darnas pat-way)
G1"co1r'5i5 (r:nroen .- .
Fate pf pyruvate 1n arcohor-ic fermentatrLon,
outrine study cf
qlyCogenesis and 'cr]-ycorenolysis. RoIe
of e\icI.e ^Mp and hormones
in grycegen. metak-.,orism. rrlr:.-=oD€ogenesis anc oentose
phosphate
pathway' outline of ths pql3bof ic siqnificance
of the above patirway-s.
.4. .z'erobic oxl Sglf cq...aId
-
,
.
lllgn9r.:tet'ics
.
Decarboxylation cf pyruvate. F.eactions o., tr:e. citric.acid._eycle"
. Calculation of .r.Tlr yield 1n anaerobic anc
aerobic oxj.dation of gfucose.
structure of the mi-tachancri- r, arrar-r('ement of electron
carriers in the
resp-i ratory chain,,substrate level- phosphorvlatiori
and cxjcatir,.e
'phosphoryration. .Tnl-,il:itors of the resciratory chain
and their action.
Brief account of the chemi-Osmotic theory of
.oxidatir.,e phraolcevlation.
BM/|4A
/
o
?
Pr
PTr-l
-
1.
BOL-rt" If
Ph.\tosynthesis
Definition of r-icr-rt - dependent a:r,c right lndepenaentreacticns. Outl-ine of cl:rcl-'ic and ncn-cyclic photophosphorylation.
Outline sttd.y of t:re C-al_rri-nrs cycle
2,
Endocrino0_:ov
.---#!4r
grief study of the sltes of blosynr-hesis
and the major physj.ological
functi'ons of insulln, glucagon, epi-nephrine, thyroxine, glucocorticoids
mineralocortj.co j-ds, anclro.iens, estroqens, gfrcwth hormone ,.
. CT,.t, TSH,
q'onadotroo-i-c hormones, vasopresson, oxvtocin, parathyrold
hormone and
cal-citonin.
3.
Metabdlj_smjf .rjg!5]s
outrine study of bbta oxidation. ca]culatjon of ,r,Tp yield
in the
beta oxj'dation of palmitic acid. outl-ine stuciy of tlre cr,rto..r-lasmic
system for fatty acj-d biosvnthesis.
Lipoprotejns
Crassification
and physiologlcal functions.
4. Metabolism of
/r.mino
lci_ds
Metabollsm of amino acids - decarloxylation, deaminatlon,
amination and transamination (two examples each) Reactions
of the
urea cycle
5. Nitroqen fixation
Nitrogen cycIe. symbj-otic nitrc-gen fixation
system.
Brq/t4/e/
nitrc
renase
,^
P,;.P.,-R 1,rr
BIecHEyrc
1
.
!
qE..rtTr.__
s_
Dr\.- c
or; DNr\,Rr)I.- '.;f DirIA
rnUL .rgrns.
Rv:p :i1. f
-?dr-inSc5ipt3 .:ncl tr.rnsl.::ti.n
Trrnscripti'-n-Bi':'sunth-'sls
of RNA' F:st tr..n;cripti,
:.-'f RN;'
n r1 proc,-.ssing
Structur.-rl
'
f r':t.rr':s :nc physi ,r
.-r.nt
-Ei:
1
functi:-)ns cf
tiiff
typ 's of RNA' r:rcfj ,p '-rf
:ncla:nus1- s,-.s -:n,
exonucru.mscs.
rcirrs.
rhc
]:".::;"i]:r:,H ;;:":;...,1
rib:-s.m3r evenrs
-""=rr..l:
^.r. .'-rno. ),:.
"
rn c.iric :t i:.ns
wirh
_n
_
;:;;,,r;:..:
::":"r:T;il:il::i"'.
3' Rec,ul ti'.n-c'f g.:nii--Jxrys1-n
:nc-r. grc.netic
-..lginecrrng
xegul rf_n
^=.pr,;t.,in bi.::synthcsi-s_ the
Lrrc -"p"r()n conc
rnducti'-,rr .::Ds-l
: :-"-"-,pt.
repr.Jssl.n (Fund
,mcrrt
maj'-,r inb'-,rn err.)rs rf pn..1 -b,rr-ism. .I stucy) . Bri,_.f stuc]y ,_1
arr., rutlin-ss of r_.crrn.b;-n
D\IA t:-chnoI:gy rnc gener.ll
-nt
.ppli"-:tions
!-': +av--L-r'-)IIU .)f
.rt ooni.ri_
goDs.tic
4. _fmmuncloqv
,rntcb::ety form:tin. :.nti9jn. tntib,:cly r
_...::ctr.
.enL structurc_., I fe
tur-.5 rf immunrrslobul
JlH,
;: l_: J: #" ;: "l I :";t rt
'
e 1.'ctroplt:'r-,:sis.
sk/I3/e
__,
-
n. Cltssific_rti .r
"
- ; ;,,,;'
ffj :,' ;uuLo-'
..ngin:-r:.-:rg.
-unrnunc
l: ",';."
i .n'. _, .,
"
Ciffusi,-,n end :rnmuno
I
P,,PER
--lO+
VfI
CLI-:I l ,
jf t.lHll{f ST?y
1.__B_!:?cr ,jl._Iysas
:nJ Hu.m.toI.
,J_y
nc.:,.f th f:.IL:wj-ngr
- / n-,n-pr:',tcrii1 nitrogi_-n, Uric
:_, _in_ r.hcnr gi-.rbin
ir-n,
d st ij_p p,: .tein r , ,)r- t ins i.l_:+-L
sphrttsc, -i-k-.Ij_n__ ph^s :i., t,s.,
_l_c.
Th=' princil'r9
.,st-im ti n lf th-. fr fc,rr::,rrinq bi.ch_mica1
prr:m-t,*.rs ,;f -rr!
blc,:d: glu- sez s:dium
_rl,_r. -.L?.
h,:mic L m.:ch-:nj.sms rf
iociplcs
f th_. folllwing
ti._:n rf th_. rlsr_tlt s: t,>tl t _ sts
_:,1..
, ,./G r ti
, .jnzynr j s tf
.>f
ic . I sigi-:ii=ic.-r oC,:) ,nu c -us,..: s
f tjr. f,-IIrrr^ling I)-f-rT.t .,t.jrs_
. ll5?, h.-.lm tccr-_i-t, &
itli;,:,c_ gf (,:u s. Stl r gs) encj
Urin-. ]:nd c-.rebr,r-s1tin I flui.,_n rrm:I
an..l _.bnl.i-m.I
c 'nrplsiL i
r i-rf r-rsq'r-ls1-. ii c1u : rit t iv-. .n : rysrs
:n.r i-nt.'apa, rt :ti:rn
Tc.sts for r,riucing subst_ncl.s j.n urin
..
3. Nutriti .n I .nd Horm .n. I .uis .l. rs
11
4. Errzrs
.2l__!!--t .bllism
Inbcrn errr.j,rs r,f glyclrgen _rntl. :rn.J.nc-, rci-<l
m.-.t b .Ij_sm , g-t I ,ct ls!-mi:.t,l
I'ctcs.-, int-tI-.r:nc,,
Hyp-,rlipicicmi,nil
:thcr ,sc l_-r.:sis .
.
f
!
t
sk/ i 3{e
I
I
I
I
I
-._...rJ
t.,r-eD\-.r v rcLyeE, cmulsifying
cmursifyins .:gents,
p:::::"rtives,
r
f i..vouring
escnts,
Elcmrnt_,:ry
Ercmrnt,:ry
stuiy 6r Ei.:i;,
stuiy
I;, il"";::i"i.;:"r:I:":.i"_g
i]n-f, ,rt .rai^re ..,"r,r;aicl
]9.]ll"r
- +_- :
_,uq!LL-!..-L.1 .,' ..r
t:ll:t:1t1.1,
"r [n,,
flccj.--3t,lcy
-'f roc..
,..t the
,.f
Stuc,,y
pq-.g6,:ds tt-; id=i;a;fy
the pq-.g6,:dS
i<,r.--nr.ir., -^,rr',
.-,stlm.tc .,JuIt..r:tiln in
c:,mm
n f,),-rrlst
.-
en.f
,r r,-,-crs by ana.--robic
-;;:;;;;.:n ,',iri.'nl"'lI'ifr=i;"3;;
,,f__p:"".rv:.ti
i
:;:iiil"":;of ::?:
.;::-:t irr
l:''. s .
prc s_.rv
ill;I.?l":n'"T::n:::
,rdoiti,',n
CI:ssific rti:n of micrc org:nisms.
c
n w tcr p:I.t-ut
purrfj-c-rti..n n.. -rsinfecti n ,,f, ,t' :.,
lt:,
w--t,Jr, s ftcning, ::nC C_.sllting ,.f wet..i,
:bs',rb:cl r xygen/ 3cDz r./r1-r. in=m ,;, ;;-i.no.tr.;lfm..nt .,f se.Irge ,
mm
3.. Tcxic'-!,
gy___n,-.
icls.rd:r_s_ _rur,_ t;
ch-.mic
-:,.L'r,rv..t
rrru p(ryqlsL_:gcnts
ic-I
!-."|"x..,"".".:r;fr.?",|.i-?}.^,.."*}-...::.-P.l1.sir;il..ffi.
l. n.
sk/ta/?
;
.
BSc 3IOCIJEI"] jTF:Y
I.IAIIJ
PRiC.IIa:*..
-t'r iT'rrY
GEl{':l
R:,.I]ES
a. .Qu'rli!_tlv.:
a-
:l.n
ll+g
Gcnerrl te cti ns ::f c-rbcl-',ziir:tgs-M: irsch's t;st, :nthr rlg: tegt,
-c:l--i t..3t.
S';eeif lc rcrcti ns
thynol test -,t: e"1€D-:J -- su) i:huric
r
cf r.:iucing s,.rg--rs- Bene.C.'ct s tcst, .:lk:liinc picr^:tc i-est,
f,erricy:.n j.rle te et, IltryI":nr: r! s test; . jlr.jrn:nstr-ti tr nf
fq:ftr-Dt-'.ti n ::.tri .'srz l;: f rrn ci n. 5i-;-cif-r.-c-f ru-c:1 ns 'f m.n' s:cch:rrcies-B:rf ,.:c-rs t-*'st" 5't-.cif-ic r-: cti- n--: 'ket',scs- s-,Iiyrn.ff ts
test . T,; :tl ns f. . '- . r;ir rd-.xtrin, ,,,cxt : nc1 glyc i.gw-D, Dem s-trrti n .;f ry,r'-rlirsis f st--rch by HCI ---:,-'. by sellv-fy trrwl -s;f solrti ,n of st. rch i=.:rrr .'.lb-.r.
b. Curnti:.tiE_.JliTli I
s
Estjmrti n ,::i r;luc :s,e by lroi:n--Wrr c -I -r-i,rn.:trlc rTr..thotr, istim-ti
.cf fruct. s" by res rcinl I-thi ur-,;: ncti', .: - Estim'ti e :f
st:rrch or E--yc:qcn by ph-.nrJ-- sulphuric'-rc i,.^ meth -1.
2. Altrlto ..crjlsj; pp,orEri{3.
S-
iLcj--.!s' -rnJ. ..j:f lmin:
n
acld r(--si(-ueg af
ct'i,'n. x:nth:pr:-:tc-!C t;st ,, MiIIcar s t.-'st .
r-1
':tc-i
t:s
t
glyo>ryIic
. I'Iitr' 'prusslcl:j test , Icli bI::ck.'nli-tg '.t -'s't .
,=st &'sak-gluchirs tcst. Gr:n,,.r:I -' r-our
EoJ.lnr C;
Rr-,cipit rti::n rc ctj.:ns f pr:;t :ins--'
reacLir ns ,f or'*-t-ins,
"
11F!
n,'::t
L
tcnsT
eth.n--I., rllc:I,-if ''I r=.:,g:nts, amm'-nium ri*
ugincr
rtecle
nc-ntr
sufptrrte,
hitr-'i-c,. tcicl. & fu'Er('Cv:nicjr:,
-::
b.Qu;rhtit:* rve i--st lT,.rt,_l+
.
'by
l?lln.ui
r.'rct1-n,
Estlm:t ..tn
ninhy*rin
.miFTc-f
Estimlt:-:,ii
,',f tyrcsini by Fr:Iin- Ci':c-It"u. re ''cti 'n. C-, I'-rrim-tric
"mcth^J, ,n.-l L:-wryt
:tcin-by biuret
L:-wrytss m-.th-C.
m-'t
biuret --mcth^Jr,n.-l
estjnrrti:n --:f pr-tcin-by
protcinsniny<1ri-r.
:: i.-'
3. LIPIDS
Rc':cti- ns ,f tr'iglycr'ri,lo-s- tc6t)"f,-r uns-fturnt+ -n, seponif;1c-t
Re:,cti,)ns .:f glyc...i,,I. Rr-, ct-i. ns .',f .s[r-:Ic'btcrol-ti*.bermlnn
Burchard t-.st;'S=rlk-.wski t - c-- 'rn,-. f ,-=rric chl:.lric-.- t-,st .
,
ut
t ivc .. st -i-m.: t i ..n
C.,I rim-tr'c cstim ti n :f ch'I:st-:r.I
n; g lyccrc,I "
4- ' M.-rt^crn ..li::ch-mic--l ',1.:chif]gll= (Dcm'nstr'ti'n onry)
b
.
Ou
i-t :
LtLt-ur.tl
Scp,.'r-rti-n r.-f tw ' sug rE r lmin., rei,J,s |312 pfp r chrcmat+gr rphy
,:r thin-I-yer chl,:rn-ti..:- ,_r
p p-r ;--ccr-.phorcsis ''f serum
prr_!c.1ns.
---
1. s:?:rm'- gy ( ,--r r-'streti
-1
only)
De.t;rrnin:t rr : ^ l,- s!;.1 ; f ic -,ctivity
f s "Iiv :rlf .-mll-s:,,
Study ': th cf : cts f sui:str-t - c -nc;rrtr -tl-;n nc', temp=r ture
,f ?fu tir,:l Vm v Iucs by
-,. te.rmin -ti;n
n.- s- Iiv .i
:I t.
crUbl' t:C-L',t
t
sk/t a/)
.-l
t
11*.
CLT}ITC:L
YllI l. .i, 3ILrcHaivir i?RY
]. _q.-i remic I -n.rIysis f lr'I -,-r,;
0ulmtit:iti-vc .-.stim.!i_n f gluclst:7.ur._
t-,L prot.:in
( s :rum ) triti -u::in-. iiil*uil
i-J cic, r_iniri, tc nt.:nt
ln blr,r,, .
".ssly t)f sg'rulTl cnzymus-, .rci. ph.'sph-t.sir , ork- lini ph.sph:t-.s_.
AIJ? -,n... ,.r:ST.
?. Urinrr snnlvsj_s
Qulli-t ,.t.ivc t:,sts tD c--t.:,Ct n,Ym..I - nci -bn rmil ci,nstitu-,nts
f
urine . ?s-S,c s f .:,r .pr rtcin , reelucing sq 3 -,f / bl I: piq;me nts
le
bi
-K..t,.,
s'r It s ,
ne b::c'l-io s tnu b I,. ,J
3 . F,-r -.1 lnr-l l.vsis
ou'erit-ti-ve.;;nd qurntit tiv- an'rysis :f mirk- tctol
u:lld.r. ,-_..,
r,:ct'.rse, erlcium -nJ c-.sL:in. an- rysi_s .r .ei=.1;:-;Jducr_nq
nnLi
n:!- r-Cucing sug-,rs.
S.;l).1:. -.ti._ r :nJ r.Stim,-tir,n )f .il.!:umin
:nc grc,burirr f-irm .99s. ,asLimltl
n .^f rc.<lucinE su,gr. in h.ncy
:1nri ,- ...'sucr- se in j rgg_.rf . Est.r_rmlti ,n .f gl1rc.:q,.ni; tlt.:I
pr';tej:r in l-ivcr" -esiimati
'r
eci_l
in
or
="..bi"
juice2 prepir,:ti .r ;f ;- j._ " ., l:ir tu:_._.rs _ri,.'cstim_-,t1.nge .rf
n
tli_.
st':rch c':nt..rt
o
D--t:rmi_n-ti
n
4 . tdtrti r :tn - 'l rrq'
Estim:t
sk/L4,/'-',)
-.
t).
CL
"L
'l
ph-.n
-,is in
t.-,
"
l
I
I
I
_sq" .OH ::.n-.r
.j-ss,_..
1.,,-,i; xy.rcn in
w'tt er
.
Pr 'ctic l_T "G. n..r _ l- tsi.rch..mistry
Pr,.ctic I-fI.Clinj_c rI & ..n,Iytic
:I
Bioch..mistry
Tw,,, R_c..rrls. (pr:ctic
,l I &If ) 2x35
Stuciy f -rur L f rt,j,t I,rss
th:n
s_:vg_h
,_l
6h
100 rn rks
6h
100 Y.,-5i1s .
70 M rks
,vs
30 M rks
___--L__
T-t -I f'.r pr.ctic I
300 trl rks
D
Pr .ctj.c .I I
.
0uusti. n f.
Iucntific
st .rch --r r
ltrr-_t in .r
substaoC.
50
m
.rks.
_
Qu-.sti tt ff . :.i qufn
pr_-f -.r
.-l
st : nrl:
supplj.e
I
,I
?h.'y h:v: t-., usc: thc s.)rn.j
Ph rt , ':l.ctric c,:;I .:_*.rlL
50 M rks.
I
I
,in turn.
l
e
a)
Thc r.rticri 1 -b:.ck gr url_1.r_. rm th-,
pr.rcj-clur- submittcd f,z g6.ass.,ss.-;l
st_u,*1jnt
i.t: / if
n!:ccss lry, by c''ir-ct .i_nt _rr:,g,ti
n.
16 m rks.
b) Prcsr.nt:ti-- n :rrl-, t .r:uI :ti n ,f
._bs_rv tj- ns
c) skill in h:ndling _f app.r_tus .nr_-:
\-.quitrrn_.nts
C) Accurcy cf rg.sul_ts
e) Numrric tI c.:Icui-ti
f) Gcn.:rlI DJ-rtnuss
ns :nci st,-.ps inv
,Ivce1
10
,rks
5 m 'rks.
10 m rks.
10 m rks.
5 m-rks.
TottI f-r prctic--,I
f.
1OO m_rks_
j=======_=====
sk/L4/s
m
(...2)
tt
lL-
rl?fC l,- if
PR-
.
Qu,
t)
sti n -f .
f -.,nti_fic
-
n. ;/ .;r
.1-l
in thc su
(sp. cify
i-!
er
,_,_
t
_--j
Ouesti:-n- II.
,
)}/ 1-1_: StU
rLi
'f
]
-,
f!i).:.',
50 m rks.
t1- rn hiS
)
I
|
,..Tu,rntit tj-v_ _.sti-m ti
nf
olj-4ic I birchcmistry,1.,r:l
-.n Iysi s r
:rm
.r,._
I
50 mat'ks.
I
I
l
SC-+Iii-:'n V i,U .ti -: .lir .iTJ-:-STJr\irr
- If
(s,:m- s f..:r uu.stj-':n-- f.l :.1 ::r-ctiClt
_ f )
f -r r--r ctic:I ff
T:trI
l4iRK-i
FOR : -
- Prctlc_.I
f
trr:ctic:I
If
R-.crrrds-pr ctic I f'
Rq.card s- pr:rctic ., I f I
Study t- ur
?.t i f ;r i r.ctlc,rI
=100+100+35+35+30=
1CO
m-rks.
1OO
marks.
100il.
35
r'
35il,
30
rr
,:
3O0 M.rr-ks.
*r(****
sk/1a/e
.-
t
Harp,:rr s ll .ch:mistry - Llurr:ry. t rl (L .ngMirc.ic .,I
npi:I _t, n ..: L.nge, pr,:ntic,,
HaIi)
1.
2.
3.
4!
5.
('
i
7
8'
9.
10.
11-'
It*";:i,1;i
f 3i
ch':r:.;istrv '-
Bi., ,ks,
-'hninE-r ct rr (ces pub-rish-'rs.
Outi'n.'s f 3i ch rrri stiy-- C5n,.{z E;E tn.J
stumpf p,K,
('dil-.y Etst.jrn vt. lt-. iv_,lv u<Iiri.)
Bi:ch:mistry
:,f hum n DiS.:s: _ H fm:n.
Dj rgn stic 3i ch-.mj.stry _ iR,.tn:., I.-, sJ rcy.
Rcviow,'-f ,{-:'-lc'r physi-r gy- G"in'ngz
. ;k, 'r.p-:.I,t n & L-ngr=., pf ;ntiC._Ui if i/.F. (r,,rr:g3 M-.cric:r
)
Hrwkr s physi:,I rgic I clr_,mistry _Os.-.r.
e.L
Text 3r'k-i'r:ch..mistry -.I{ rr.-w & Mrzur (s-runc^._.rf s)
Bi:ch;mistry _ Orten & N-uh.rit.--:s (c.v.tullsby
C1.,.)
f ntr._,uuct j- n t: Bi :ch..mistry _ Sutti:;
:
Cr-rncls' Tuxt B:-r k rrf Bi-ch-mistry
-T.r{. F:ttrbirrmf
(aff fnL*i.r publish.__rs a Distribut..,rsr
Ch.:nn_i_
n
B4)
72
-
13.
74'
15.
16.
17.
18'
19.
Princii-rr:s :;f g1..s6,Ti:!.y
-,G rr*,r^:r. ,,sp:cts smith,
E.L. .-,t :I (tIc Crrw HilI i.:,:l<
M,:mm-Ii:n tsie,ch:mj_stry Smith"",-l
E.L. c.t tI (Mc Gr-rw Hill
,3,-' k C:'. )
Tcxt 'r- 'l< ':f Bir:ch-:mistry f r j,1-,^ic
V-,suc-lcv-n & S. src.,kum^rl_ (J-yp_:-!r i stu,J--nts - D.M.
th..rs, Mjrlic._l
.,f Bi _,ch..mistry _ U, Slthyin ,r,..ry-)n.':
T-.xt B,.k f .Mc,_.j_c:L li gh_rnistry._ M_.N.Ch _ttcrie
nrh' shin,,c (J;t6:.j'ar6tt,i"'l-fi'._ri. r pu,ciiJ[_1;,: :n,f
iVe ur Dclhi - Z.
Text B k .f tsi.ch_,mis1_ry _ D-b
B:r,.tk
pr ctic r Crinic i -i_"h:*istry (v
r. r & fr )_\rrrreyz
Hi G,:wcnI,ck, A.H. & 3cii-, l{. (C.S.S pui:lish..rs,
iy,:w D..Ihi)
R
Inst .nt ltr t -s j_n Bi ch.mistry _ H-.1 ,s,
B/D. t lf , ;) r /
N'lui' & .J ught-n, J.D. (ei- s scicntrri.
pubrish_lrs,
oxf-rd
Viv B: ks pvt. LTD. fJ_.r.; D_.Ihi)
sk/7a/e
c
F
20.
27.
22.
D
24.
25.
26.
z/.
?a).)
-
2:ji ch.:mistry : Th: N.rti ,n -r
rn_t sirtm
r s_r
rn, D.B . (a. r,v v .rr,iyl*(_.ic
. vr , -..,.r;_lJ :_y*i_ll,r""
Ll
Tcxt B_, k r,f M_.i11",1
L.b r_,t ry Tochi I
Gr:,^k-'r (tsh-,r.:ni pubi..r-,i"q
H'rl ,'i'._nn lrTo - Fr-fur.B.
-,n Intrr,:duct-i^n
pr ct_ic ,l Bi ,ch:mi-st_
D'T- (T'ta Mc Gr,*t I,riirl'*-,I*'.1i*:n:mi-st-rv
D;rhi --- -- Frurnmcr,
Micr. -n,,ry.i]';l
pr,,ctic l crinic
,2
t
;:ji:,T.',
r1 Enzrzm:.r_"j;"::I;trv
-Kins & iv :r:,n.
C1inictl Lrb r.-t
ry M.:th__.:_;s & Dj qn.sis
A.O.;,.C- Offjci I
M..th,:,_.is ,f
n.ivsis
Thc Ch_.mic I. l.n:lysIs ,..f
F:,.,,1 ;M rri-.s.V
ch.-.trric I ,n-:
lysis f F ,-.i_ n-vj
' P- r-s
r,r..,7 lrti
30.
31.
32.
;;
:t;',
r,xic:I,-,ey
=G
Gr.cj wrhl
J_.sa,|
n-
Ir rf
'".,1
:r ",
st
??
tF-
,i.lii"l
"'Jurt.-,r''r--.i- ,n
,rct ( ,rmy cuc:ti
n_rr
Mir-'1':fi' iosv pr'-sc')t
& Dcnn q.,.cv trublic:ti.,ns)
j.i.ij;Ji'l"sv -. Macfti-' & Mc c rrthy
1'_;. B._,k,r^
''r 5l- ;ch',lllistry
Brugg-.n -'- i/-.st, Tc,r-lj, M-rs n & van
Physic.]I Ch.:mistry _Drnicls
c ,.lb-.r.ty, (l,Iilcy)
-
34.
35.
36.
37.
38.
39.
40.
47.
I_'gv: th-- b-sic
-*v .scic
.or-rCrlcL 'f :.ri._ts.ns_
C:s r_,tt
l:'T'-",
an-.1
D,rtfls.
Met}l6':J,-'1':,,9y ..6
an.rlytic-. l ti_vi^._.r
-oxic':rr:gy
- lrving sunshinc.
rncustri. f F-.rrlg.nt
ti-n_ Uni-riE ft_,r
& Hick._,y
_u.
sk/L4//9
**rk**)t****
- D lvicl
pe
trs )n.
...\
UNTVIRSTTY
OF
FIRST YI R B.S c )IGRf,I I)..I1IN ,ITON
PnRT
P \PER
III-
,Bf oCHi]T,iTSTRY
C r, ,rSp:.,-CTS
_1!;_PF[qf
MEIHO.DS f
N 3rOCI]
Tim.--;Thr..,:Hurs
z\nsw;r m-y bc rvritt:n
(IT.riv)
'i:
]'JT ].Y "
M-:xirnr-rr-n
.:i_thcr in Enqlish
S lf CTi Oi:.'t
:-
.]rlD
z
65 l,t rks.
:,.r- iru I,l -Ll -y .I:m
i.. .
-- ;.
1.
2.
3.
4.
5.
r.- thc -rrr:ngi:m-:nt ,-,f e lcctf L)nS, pr _tons :.n<.1
n;utr. ns in th_. thr_,i_. is ,t ,p.--s f hyJr.,giln.
C, ml:' ::_ thc n t ur-. tnil
m-r
r prlpcrtics ::f tlpn., b.-t.r :nci
g mm.' lryS.
Glve -n cx .mpl-. f ,tr th-, usc ,-:,f s is<rtc;pic tr ,ccrs
i.n
bi cir_.mi_strv
C-IcuI t,_ the pH v,,,Iue :>f .-'r.0.01 N soluti -n ,..f srcium
hyrlrcxii_le .i-n w:tts-r.
C,:.mp
-i
N-m='
thc typcs r-,f clcctr.-tl,:S
uS._.,.-
in - pH M_.t.r.
st te th': Hc.ncrers.)n- Hass ,rbarch \rqurti n.
N--rn.: rf - or.
::,f its :pplic tj_,-rns.
7. D.:fin., thc pKa v,Iu,. ,.f tlt :ciu.
H,,!v is j_t Fi, I t._.., t
th-- Strength cf tn :cij.
8.
H:w wiII ytru pr,.p:.r.,- tw,r Iitrcs .:f 0.1 X )rm,_,
I soluti n
:',f s rrlium c-)r'b.\n.t. 1n w-,t:r ?
9.
CrlcuI :tc the ,,rsm,-tic prr-.ssur,.
. 1_.g/. s._, luti .n of
glucc,sc :t 21 c (R=0.0821 litr-, f :tm,-.=fh_..-_";.
10. of th,, tw,: soluti ns_ 1% qluccs_,
n_: l%:Ibuminr rdhlch
. '.x:rts hir7h,-''r ::smotic pr ]sSur-? lrhy?
''
11.
Titrich ,_.f the
pr,;rssurL
fr;Ilwing scluti-ns
wcul,_
..X rt h1gh.r csm-rtic
fC S(ln.
72.
!"rr.it.. th,_. .rXprr.ssi n f._.r thc rctui librium
crn st :.nt .)f
_iric i r.:.lct i .n .
A+B ,
C+D.
ilIh tt is ..n L:mulsirn? rVrm.-. rn
cmulsifying _iq,*.nt.
Cl:ssify chrom ,t rgr phy . tir., b ,sis
-:f ,-rincipr.: -,f
sup:r-rti o.
ch
13.
14.
sk/7a/e
thr_-
t n-.
r..o15.
,.
16 .
I
c'>
ca-
-
7'7.
18'
th_ .:,j,t :rts usccl in zan I :1
ctr-:,:h-:r _.sis
'?.|.';"lh,, rp.clic ti . f stu- -,nt=' ,t, t.',st in
79.
lI
20-
Outiin_: _n\ .:h n: ,
I fS quc*sti n.i. 1 m _r:k = 15 m-rks
N.rrnc
,m:, t,,
r.:1._. 1:,.n
I [i
.ls
2L.
D;fi.nu th: t.-i-ms
22'
1?;lr.t:.?^l:r'
23.
bi_ ,chc,mierr
c :ii-cut_r n t work.
s_: ring t.chni..u_..
-
r-'
rt_:
-lr'l
-
)
3
.n_rEy , *.nth llpy
nc: cntr^^py.
csuiribrium ? I'/h-t is its bi.,:ch-,mic-r
fr-,.
Distinguish b.rt.w,,en tru.:.sr Iuti_!sr -,Iir.ii
c.:rs., sus[i.nsi,.i:. lt :w-iJ i*i,-,f
I s lrti ns n:..
_c
i.,i::l-i:
i,.rtir
Wh.-rt is tyn.J II ..ffect? ,,,^nt
st:bit-is -,i_l?.
a ii" its .nplic_ti nns?
Distinguish b.:t,;vc" n fy .;,hiIic -.,nr,
T),:..phc,lcic cr..IIoicls. Gj_vr,
an cx,:mplc f .r _^r Ch tylr:,.
24.
25,
l1
Dcfin. RF V Lu-. i,le t is its
epplic ,ti..,n?
Dcf in - :r Ic.Ct r.-,ph.)r _,tic
m:.bi lity .
ih .t .:r!: t h; f lctilrs
tffcciln.rt?
Bri,,f Iy _x; i,j-n h,rw .l mixturi_.
LW lml-ni- lCl.:s C_n b,:
scp:r ttcd bv thin_ I,,y-.r chr mcft .qr
phy,
outllni th.r princii:l_, ,ni_L :pplic j- 'ns
t
-: rn.-1 I e cul.:r
sieving
outlin- tlc principl.- -n c.)nstruct_-:
n f .Ln urtr
c ccntrifugc outlin,_ thc eppijs-1 i :ns
-.f ..rfL c,-,ntrifu_
g:-.ti n j_n biochcrnistry.
,'.h ,lt is ui-_Llysi-s?
M nti
:pr:Ij_c rti n r)f ,,,i_ Iye is,
;xpl-ining th. nrinclpl . n-n nJ
,. 5 ,,- ...-,.
.,f,h :t is th_ principl_:.
_f
_mc._nstructi n ,nu :i:plic--ti f I ns. phct:;m_.try? ExpI:in its
!--
26.
27
'
28.
29.
30.
Bxpl-in th-' ph:n-m:n f frur-'sc.,c; -'ncl
th-. '-,rincipl. i ff".i..til;
_,,^h:tph-,sph,. r,scence .
r.r. its lpplic_t.i
1'rincipI. c,f stuc _.nt s , t, t st
in ,ci ,l.,sic
i:::"::n:n',t,'hrt i-s
31.
r:.
32.
-'t .rri: thc p::j_ncip1.
s n -r -rtpljsrgl ns .rf c_rr I_,ti
cccf ficicnt :ni_l r_,gr._,ssi
n-c .-:f ficicnt?
Wh
sk/ta/9
c.nt,....
n
ns?
t3.
33.
Outlj-n. th: cliff-l
Jo r-
J
a
,,nt typ_.s ,:_f ,mem.-)ric.s us-; in
'v'.Ih tt
tr_. its -lpplic-.ti ns?
34.
Clmput,..r systems,
I.Ih'-t is
rrr:.r-t.:rrr?
35.
Enuncrtte diffg'fg-flt 1.:yers of c r)m,Jut_r n-,twirk.
( tO qU:stl: ns x 3 m.rks = 30 m-.rks )
SEC?IC}I - C.
Answer :ny
tw,.--. questi-)ns f ,rm suctl ,n - C.
6:ch qucsti,n c-.rri.-s t_.n m-:rks.
36. Outline the- sh:pcs ,rf titrrti.;n curv-.s cbt incd whc-n
.:r s,-Iuti::n cf scdium hyclr>xiCc. is titr-itcr_. rgeinst
(':) tryclrcchrcr.ic aci.i :-rrr,.-i ( b) .-rc..tlc .e,ciil. Expr.r jp thc
lcuffering lcti-'n :rf ': mixturc 'f sciliu m ecet.:t,-: -rnd
-rc-tic acici. i,rlh-t -r._. th.: f ct.,rs tf:_cccting buffcr
crp-city?
37. ;lLr.t lr: thc gsfl,rrl ch,r']ct._,ristics :f crt-.Iysed
fC,-:Ct j- -nS?
D-.fj-n-. ,.t-rsitiv-,
n-.g.'tirz: :ntl aqt _-. C::t.,IySiS with
. :rn cxempl- :. ch. Expl:,in th.r .cti n r.f pr.m.t.lrs :nC
c :l- Iytic p:is'',ns.
38.
St.t,, B.:.:f- Lirmb-.rt's I-.w. I,,Ih t is th: r:lrti D bi,tw.-,n
-rbs-.rb ncc nc, c..ncentrrtirn
tr-lnsmittrncj,
f s:ruti ns?
C' mprr-' th; eansti'ucti n ',nc n-:'r|js11i n rf photcrelectric
cl I._-,rirn.-t.:r's anc'l & socctr,.,phr)tomct _.rs .
--i
sk/L4/e
( 2 quest1 ns x 10 m,rks:
********
2Om
rks).
I
rl
ri
i
I
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