Curriculum Vitae Name: Date of Birth:

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Curriculum Vitae Name: Date of Birth:
JE Segall cv
Curriculum Vitae
Date of Birth: March 30, 1957
Harvard College, Cambridge, MA
California Inst. Technology, CA
Biology and Physics
Post-graduate Training:
Postdoctoral Fellow, Department of Cell Biology
Max Planck Institute for Biochemistry, W. Germany
Professional Employment:
Assistant Professor, Department of Anatomy and Structural Biology
Albert Einstein College of Medicine, Bronx, NY
Associate Professor, Department of Anatomy and Structural Biology
Albert Einstein College of Medicine, Bronx, NY
2000- present Professor, Department of Anatomy and Structural Biology
Albert Einstein College of Medicine, Bronx, NY
Professional Society Membership:
Since 1993
American Society of Cell Biology
Since 1997
American Association for Cancer Research
Since 1998
American Association for the Advancement of Science
Awards and Honors:
National Science Foundation Predoctoral Fellowship
Arthur McCallum Fellowship
Max Planck Society Fellowship
NATO Postdoctoral Fellowship
EMBO Short-term Fellowship
Mellam Family Foundation Investigator Award, American Heart Association, NYC
Established Scientist Award, American Heart Association, NYC Affiliate
Vice-Chair for Gordon Conference on Sensory Transduction in
Microorganisms for 2000
Chair for Gordon Conference on Sensory Transduction in
Microorganisms for 2002
Vice-Chair for Gordon Conference on Gradient Sensing and Directed Cell Migration
Chair for Gordon Conference on Gradient Sensing and Directed Cell Migration 2007
Betty and Sheldon Feinberg Senior Faculty Scholar in Cancer Research
Speaker, Einstein Faculty Senate
JE Segall cv
Original Communications in Reviewed Journals
Chiang, P.K., Guranowski, A. and J.E. Segall (1981) Irreversible Inhibition of SAdenosylhomocysteine Hydrolase by Nucleoside Analogs. Arch. Bioc. Bioph. 207:175:184.
Segall, J.E., Manson, M.D. and H.C. Berg. (1982) Signal Processing Times in Bacterial
Chemotaxis. Nature 296:855-857.
Block, S.M., Segall, J.E. and H.C. Berg. (1982) Impulse Responses in Bacterial Chemotaxis. Cell
Segall, J.E., Ishihara, A. and H.C. Berg. (1985) Chemotactic Signaling in Filamentous Cells of
Escherichia coli. J. Bacter. 161:51-59.
Segall, J.E., Block, S.M, and H.C. Berg. (1986) Temporal Comparisons in Bacterial Chemotaxis.
Proc. Natl. Acad. Sci. USA 83:8987-8991.
Segall, J.E., Fisher, P.R. and G. Gerisch. (1987) Selection of Chemotaxis Mutants of Dictyostelium
discoideum. J. Cell Biol. 104:151-161.
Segall, J.E.. (1988) Analysis of Responses of Dictyostelium discoideum Amoebae to
Chemoattractant Concentration Changes. J. Muscle Res. Cell Mot. 9:481-490.
Segall, J.E., Bominaar, A., Wallraff, E. and R. de Wit. (1988) Analysis of a Dictyostelium
Chemotaxis Mutant with Altered Chemoattractant Binding. J. Cell Sci. 91:479-489.
Andre, E., Brink, M., Gerisch, G., Isenberg, G., Noegel, A., Schleicher, M., Segall, J. E., and E. A.
Wallraff. (1989) A Dictyostelium Mutant Deficient in Severin, an F-actin Fragmenting Protein,
Shows Normal Motility and Chemotaxis. J. Cell Biol. 108:985-995.
Brinke, J., Gerisch, G., Isenberg, G., Noegel, A.A., Segall, J.E., Wallraff, E., and M. Schleicher.
(1990) A Dictyostelium Mutant lacking an F-actin Crosslinking Protein, the 120 kD Gelation Factor.
J. Cell Biol. 111:1477-1489.
Barisic, K., Mollner, S., Noegel, A. A., Gerisch, G., and J. E. Segall. (1991) cDNA Sequence of
Cyclophilin from Dictyostelium discoideum. Developmental Genetics, 12:50-53.
Segall, J.E. (1992) Behavioral responses of streamer F mutants of Dictyostelium discoideum:
effects of cyclic GMP on cell motility. J. Cell Sci., 101:589-597. Segall, J.E. and G. Gerisch.
(1989) Genetic Approaches to Cytoskeleton Function and the Control of Cell Motility. Ann. Rev. in
Cell Biology: Cytoplasm and Cell Motility. 1:44-50.
Segall, J.E. (1993) Polarization of yeast cells in spatial gradients of mating factor. Proc. Natl.
Acad. Sci. 90:8332-8336.
Segall, J.E., Kuspa, A., Shaulsky, G., Ecke, M., Maeda, M., Gaskins, C., Firtel, R.A., and W.G.
Loomis. (1995) A MAP kinase necessary for receptor-mediated activation of adenylate cyclase in
Dictyostelium. J. Cell Biol. 128:405-413.
Segall, J.E., Tyrech, S., Boselli, L., W., Masseling, S., Chan, A., Helft, J, Jones, J. and J.
Condeelis. (1996) EGF stimulates lamellipod extension and chemotaxis in metastatic mammary
adenocarcinoma cells by an actin-dependent mechanism. Clin. Exp. Met. 14:61-72.
Gaskins, C., Clark, A.M., Aubry, L., Segall, J.E., Firtel, R.A. (1996) The Dictyostelium MAP kinase
ERK2 regulates multiple, independent developmental pathways. Genes and Dev. 10:118-128.
Knetsch, M.L.W., Epskamp, S.J.P., Schenk, P.W., Segall, J.E., and B.E. Snaar-Jagalska. (1996)
Both G-protein and ras-dependent activation of extracellular signal regulated kinase 2 is
antagonized by intracellular cAMP in Dictyostelium discoideum. EMBO J. 15:3361-3368.
Leberer, E., Wu, C., Leeuw, T., Fourest-Lieuvin, A., Segall, J.E., and D. Y. Thomas (1996)
Functional characterization of the Cdc42p binding domain of yeast Ste20p protein kinase. EMBO
J. 16:83-97.
Edmonds, B.T., Wyckoff, J., Yeung, Y.-G., Wang, Y., Stanley, E.R., Jones, J., Segall, J.E., and J.
Condeelis (1996) Elongation factor-1alpha is an overexpressed actin binding protein in metastatic
rat mammary adenocarcinoma. J. Cell Science 109:2705-2714.
Chan, A., Raft, S., Bailly, M., Wyckoff, J., Segall, J. and Condeelis, J. (1998) EGF stimulates an
increase in actin nucleation and filament number at the leading edge of the lamellipod in mammary
adenocarcinoma cells. J. Cell Sci. 111:199-211.
Wang, Y., Liu, J. and J.E. Segall (1998) MAP kinase function in amoeboid chemotaxis. J. Cell Sci.
JE Segall cv
22. Wang, Y. and J.E. Segall (1998) The Dictyostelium MAP kinase DdERK2 functions as a cytosolic
protein in complexes with its substrates in chemotactic signal transduction. Biochem. Biophys.
Res. Comm. 244:149-155.
23. Bailly, M., Yan, L., Whitesides, G.M., Condeelis, J.S., and J.E. Segall (1998) Regulation of
protrusion shape and adhesion to the substratum during chemotactic responses of mammalian
carcinoma cells. Exp. Cell Res. 241:285-299.
24. Farina, K., Wyckoff, J., Rivera, J., Lee, H., Segall, J., Condeelis, J. and Jones, J. (1998) Cell
motility of tumor cells visualized in living intact primary tumors using green fluorescent protein.
Cancer Res. 58:2528-2532.
25. Wyckoff, J.B., Insel, L., Khazaie, K., Lichtner, R.B., Condeelis, J.S. and J.E. Segall (1998)
Suppression of ruffling by EGF in chemotactic cells. Exp. Cell Res. 242:100-109.
26. Bailly, M., Macaluso, F., Cammer, M., Chan, A., Segall, J.E., and J.S. Condeelis. (1999)
Relationship between Arp2/3 complex and the barbed ends of actin filaments at the leading edge
of carcinoma cells after EGF stimulation. J. Cell Biol. 145:331-345.
27. Hill, K., Welti, S., Yu, J., Murray, J.T., Yip, S., Condeelis, J.S., Segall, J.E., Backer, J.M. (2000)
Specific requirement for the p85-p110alpha PI 3-kinase during EGF-stimulated actin nucleation in
breast cancer cells. J. Biol. Chem. 275:3741-3744.
28. Chan,A.Y., Bailly,M., Zebda,N., Segall,J.E, and J.S. Condeelis (2000) Role of cofilin in epidermal
growth factor-stimulated actin polymerization and lamellipod protrusion. J Cell Biol. 148:531-542.
29. Wyckoff, J.B.,Jones, J.G., Condeelis, J. C., and J E. Segall (2000) A critical step in metastasis: In
vivo analysis of intravasation at the primary tumor. Cancer Res. 60:2504-2511.
30. Wyckoff, J.B., Segall, J.E. and Condeelis, J.C. (2000) The collection of the motile population of
cells from a living tumor. Cancer Res. 60:5401-5404.
31. Bailly,M. , Wyckoff,J.B., Bouzahzah, B., Hammerman,R., Sylvestre,V., Cammer,M., Pestell,R.G.,
and J. E. Segall (2000) Distribution of the EGF receptor in chemotaxing adenocarcinoma cells.
Mol. Biol. Cell. 11:3873-3883.
32. Hill, K.M., Huang, Y, Yip, S.-C., Yu, J., Segall, J.E., and J.M. Backer. (2001) N-terminal domains of
the class IA phosphoinositide 3-kinase regulatory subunit play a role in cytoskeletal but not
mitogenic signaling. J. Biol. Chem. 276:16374-16378.
33. Bailly, M., Ichetovkin, I., Grant, W., Zebda, N., Machesky, L.M., Segall, J.E., and J. Condeelis
(2001) The F-actin side binding activity of the Arp 2/3 complex is essential for actin nucleation and
lamellipod extension. Curr. Biol. 11: 620-625.
34. Bouzahzah, B., Albanese, C., Ahmed, F., Pixley, F., Lisanti, M. P, Segall, J.E., Condeelis, J.,
Joyce, J., Minden, A, Der, C.J., Chan, A., Symons, M. and R. G. Pestell. (2001) Rho Family
GTPases Regulate Mammary Epithelium Cell Growth and Metastasis Through Distinguishable
Pathways. Mol. Med. 7: 816–830.
35. Wang, W., Wyckoff, J.B., Frohlich, V.C., Oleynikov, Y., Huttelmaier, S., Zavadil, J., Cermak, L.,
Bottinger, E.P., Singer, R.H., White, J.G., Segall, J.E., and J.S. Condeelis. (2002) Single cell
behavior in metastatic primary mammary tumors correlated with gene expression patterns
revealed by molecular profiling. Cancer Res. 62:6278-88.
36. Vallier, L.G., Segall, J.E., and M. Snyder. (2002) The alpha-factor receptor C-terminus is important
for mating projection formation and orientation in Saccharomyces cerevisiae. Cell Motil
Cytoskeleton 53:251-66.
37. Butty, A.C., Perrinjaquet, N., Petit, A., Jaquenoud, M., Segall, J.E., Hofmann, K., Zwahlen, C., and
M. Peter. (2002) A positive feedback loop stabilizes the guanine-nucleotide exchange factor
Cdc24 at sites of polarization. EMBO J 21:1565-76.
38. Ahmed F, Wyckoff J, Lin EY, Wang W, Wang Y, Hennighausen L, Miyazaki J, Jones J, Pollard
JW, Condeelis JS, Segall JE. (2002) GFP Expression in the Mammary Gland for Imaging of
Mammary Tumor Cells in Transgenic Mice. Cancer Res. 62:7166-9.
39. Wang W, Wyckoff JB, Wang Y, Bottinger EP, Segall JE, Condeelis JS. (2003) Gene expression
analysis on small numbers of invasive cells collected by chemotaxis from primary mammary
tumors of the mouse. BMC Biotechnol. 3:13-25.
40. Kempiak, S., Yip, J.-L., Backer, J., and J. E. Segall (2003) Local signaling by the EGF receptor. J.
Cell Biol.162:781-787.
41. Wyckoff J, Wang W, Lin EY, Wang Y, Pixley F, Stanley ER, Graf T, Pollard JW, Segall J,
Condeelis J (2004 ) A paracrine loop between tumor cells and macrophages is required for tumor
cell migration in mammary tumors. Cancer Res. 64:7022-9.
42. Wang W, Goswami S, Lapidus K, Wells AL, Wyckoff JB, Sahai E, Singer RH, Segall JE,
Condeelis JS. (2004) Identification and testing of a gene expression signature of invasive
carcinoma cells within primary mammary tumors. Cancer Res. 64:8585-94.
JE Segall cv
43. Kempiak SJ, Yamaguchi H, Sarmiento C, Sidani M, Ghosh M, Eddy RJ, Desmarais V, Way M,
Condeelis JS, Segall JE (2005) J. Biol. Chem. 280:5836-5842.
44. Goswami S, Sahai E, Wyckoff J, Cox D, Pixley F, Stanley ER, Segall JE, Condeelis, J (2005)
Macrophages promote the invasion of carcinoma cells in vitro via a paracrine loop. Cancer Res.
45. Yamaguchi H, Lorenz M, Kempiak S, Sarmiento C, Coniglio S, Symons M, Segall J, Eddy R, Miki
H, Takenawa T, Condeelis J. (2005) Molecular mechanisms of invadopodium formation: the role
of the N-WASP-Arp2/3 complex pathway and cofilin. J Cell Biol. 168(3):441-52.
46. Sahai E, Wyckoff J, Philippar U, Segall JE, Gertler F, Condeelis J. (2005) Simultaneous imaging
of GFP, CFP and collagen in tumors in vivo using multiphoton microscopy. BMC Biotechnol. 5:1422.
47. Soon L, Mouneimne G, Segall J, Wyckoff J, Condeelis J. (2005) Description and characterization
of a chamber for viewing and quantifying cancer cell chemotaxis. Cell Motil Cytoskeleton. 62(1):2734.
48. Xue C, Wyckoff J, Liang F,Sidani M, Violini S, Tsai K-L, Zhang Z-Y, Sahai E, Condeelis J, Segall
JE (2006) EGF receptor overexpression results in increased tumor cell motility in vivo coordinately
with enhanced intravasation and metastasis. Cancer Res. 66:192-7
49. Xue C, Liang F, Mahmood R, Vuolo M, Wyckoff J, Qian H, TsaiK-L, Kim M, Locker J, Zhang Z-Y,
Segall JE (2006 ) ErbB3-dependent motility and intravasation in breast cancer metastasis. Cancer
Res. 66: 1418-1426.
50. Chen S, Segall JE (2006) EppA, a putative substrate of DdERK2, regulates cyclic AMP relay and
chemotaxis in Dictyostelium discoideum. Eukaryot Cell. 5:1136-46.
51. Wyckoff JB, Wang Y, Lin EY, Li JF, Goswami S, Stanley ER, Segall JE, Pollard JW, Condeelis J.
(2007) Direct visualization of macrophage-assisted tumor cell intravasation in mammary tumors.
Cancer Res. 67:2649-56.
52. Wang W, Wyckoff JB, Goswami S, Wang Y, Sidani M, Segall JE, Condeelis JS. (2007)
Coordinated regulation of pathways for enhanced cell motility and chemotaxis is conserved in rat
and mouse mammary tumors. Cancer Res. 67:3505-11.
53. Pu J, McCaig CD, Cao L, Zhao Z, Segall JE, Zhao M. (2007) EGF receptor signalling is essential
for electric-field-directed migration of breast cancer cells. J Cell Sci. 120 :3395-403.
54. Kedrin D, Gligorijevic B, Wyckoff J, Verkhusha VV, Condeelis J, Segall JE, van Rheenen J (2008)
Intravital imaging of metastasis through an orthotopic Mammary Imaging Window. Nature
Methods. Nature Methods. 5:1019-21
55. Hernandez L, Smirnova T, Kedrin D, Wyckoff J, Stanley ER, Cox D, Muller WJ, Pollard JW, van
Rooijen N, Segall JE (2009) EGF/CSF-1 Induced Breast Cancer Cell Invasion is Triggered by
Heregulin Beta 1 and CXCL12. Cancer Res. 69:3221-7.
56. Kedrin D, Wyckoff JB, Hynes NE, Arteaga CL, Segall JE (2009) ERBB1 and ERBB2 have distinct
functions in tumor cell invasion and intravasation. Clin. Cancer Res. 15: 3733-9.
57. Gligorijevic B, Kedrin D, Segall JE, Condeelis J, van Rheenen J. (2009) Dendra2 photoswitching
through the Mammary Imaging Window. J Vis Exp. 28:1278.
58. Pang H, Flinn R, Patsialou A, Wyckoff J, Roussos ET, Wu H, Pozzuto M, Goswami S, Condeelis
JS, Bresnick AR, Segall JE, Backer JM. (2009) Differential enhancement of breast cancer cell
motility and metastasis by helical and kinase domain mutations of class IA phosphoinositide 3kinase. Cancer Res. 69(23):8868-76.
59. Piatkevich KD, Hulit J, Subach OM, Wu B, Abdulla A, Segall JE, Verkhusha VV. (2010) Monomeric
red fluorescent proteins with a large Stokes shift. Proc Natl Acad Sci U S A. 2010 Mar
60. Hernandez L, Magalhaes MA, Coniglio SJ, Condeelis JS, Segall JE: Opposing roles of CXCR4
and CXCR7 in breast cancer metastasis. Breast cancer research : BCR 2011, 13(6):R128.
61. Medina-Ramirez CM, Goswami S, Smirnova T, Bamira D, Benson B, Ferrick N, Segall J, Pollard
JW, Kitsis RN: Apoptosis inhibitor ARC promotes breast tumorigenesis, metastasis, and
chemoresistance. Cancer research 2011, 71(24):7705-7715.
62. Loudig O, Brandwein-Gensler M, Kim RS, Lin J, Isayeva T, Liu C, Segall JE, Kenny PA,
Prystowsky MB: Illumina whole-genome complementary DNA-mediated annealing, selection,
extension and ligation platform: assessing its performance in formalin-fixed, paraffin-embedded
samples and identifying invasion pattern-related genes in oral squamous cell carcinoma. Human
pathology 2011, 42(12):1911-1922.
JE Segall cv
63. Boimel PJ, Cruz C, Segall JE: A functional in vivo screen for regulators of tumor progression
identifies HOXB2 as a regulator of tumor growth in breast cancer. Genomics 2011, 98(3):164-172.
64. Roussos ET, Balsamo M, Alford SK, Wyckoff JB, Gligorijevic B, Wang Y, Pozzuto M, Stobezki R,
Goswami S, Segall JE et al: Mena invasive (MenaINV) promotes multicellular streaming motility
and transendothelial migration in a mouse model of breast cancer. Journal of cell science 2011,
124(Pt 13):2120-2131.
65. Smirnova T, Adomako A, Locker J, Van Rooijen N, Prystowsky MB, Segall JE: In vivo invasion of
head and neck squamous cell carcinoma cells does not require macrophages. The American
journal of pathology 2011, 178(6):2857-2865.
66. Henkels KM, Farkaly T, Mahankali M, Segall JE, Gomez-Cambronero J: Cell invasion of highly
metastatic MTLn3 cancer cells is dependent on phospholipase D2 (PLD2) and Janus kinase 3
(JAK3). Journal of molecular biology 2011, 408(5):850-862.
67. Boimel PJ, Smirnova T, Zhou ZN, Wyckoff J, Park HI, Coniglio SJ, Patel P, Qian BZ, Stanley ER,
Bresnick AR et al: Contribution of CXCL12 secretion to invasion of breast cancer cells. Breast
cancer research : BCR 2012, 14(1):R23.
68. Coniglio SJ, Eugenin E, Dobrenis K, Stanley ER, West BL, Symons MH, Segall JE: Microglial
stimulation of glioblastoma invasion involves EGFR and CSF-1R signaling. Molecular medicine
(Cambridge, Mass) 2012.
69. Harris T, Jimenez L, Kawachi N, Fan JB, Chen J, Belbin T, Ramnauth A, Loudig O, Keller CE,
Smith R et al: Low-Level Expression of miR-375 Correlates with Poor Outcome and Metastasis
While Altering the Invasive Properties of Head and Neck Squamous Cell Carcinomas. The
American journal of pathology 2012, 180(3):917-928.
70. Smirnova T, Zhou ZN, Flinn RJ, Wyckoff J, Boimel PJ, Pozzuto M, Coniglio SJ, Backer JM,
Bresnick AR, Condeelis JS et al: Phosphoinositide 3-kinase signaling is critical for ErbB3-driven
breast cancer cell motility and metastasis. Oncogene 2012, 31(6):706-71
B. Books, Chapters in Books, and Review Articles
Segall, J.E. and G. Gerisch (1989) Genetic approaches to cytoskeleton function and the control of
cell motility. Curr. Op. Cell Biol.: Cytoplasm and Cell Motility. 1:44-50.
Gerisch, G., Segall, J.E. and E. Wallraff. (1989) Isolation and Behavioral Analysis of Mutants
Defective in Cytoskeletal Proteins. Cell Motility and the Cytoskeleton. 14:75-79.
Segall, J. E. (1990) Mutational Studies of Amoeboid Chemotaxis using Dictyostelium discoideum.
In: Biology of the Chemotactic Response, (J. Armitage and J. Lackie, eds.) Cambridge University
Press, Cambridge, pp 241--272.
Jones, J., Segall, J.E. and J. Condeelis. (1991) Molecular analysis of amoeboid chemotaxids:
parallel observation in amoeboid phagocytes and metastatic tumor cells. In Cytokines and Cell
Motility (I. Goldberg and E. Rosen, eds.) Birkhauser Verlag, New York.
Manson, M.D., and J.E. Segall (1992) Chemotaxis. In The Encyclopedia of Microbiology (J.
Lederberg, ed.) Academic Press, San Diego.
Condeelis, J., Jones, J., and J. E. Segall (1992) Chemotaxis of metastatic tumor cells: clues to
mechanisms from the Dictyostelium paradigm. Cancer and Metastasis Reviews 11:55-68.
Mann, S. P. Devreotes, S. Eliott, K. Jermyn, A. Kuspa, M. Fechheimer, R. Furukawa, C. Parent, J.
Segall, G. Shaulsky, P. Vardy, J. Williams, and R. Firtel. (1994) Cell biological, molecular genetic,
and biochemical methods to examine Dictyostelium. In: "Cell Biology: A Laboratory Handbook"
(ed J. Celis), Academic Press 1:412-451.
Cammer, M., Wyckoff, J. and J.E. Segall (1997) Computer-assisted analysis of single cell motility.
In: Basic Cell Culture Protocols (eds. J.M. Walker and J.W. Pollard), Methods in Molecular
Biology, 75:459-470.
Zigmond, S.H., Foxman, E.F. and J.E. Segall (1998) Chemotaxis Assays for Eukaryotic Cells. In:
Current Protocols in Cell Biology (eds) Bonifacino, J.S., et al. New York, John Wiley & Sons. (in
JE Segall cv
10. Bailly, M. Condeelis, J. and Segall, J.E. (1998) Chemoattractant-induced lamellipod extension.
Microscopy Research and Technique. 43:433-443
11. Segall, J.E. (1999) Cell Polarization: Chemotaxis gets cracking. Current Biology 9:R46-R48.
12. Pestell, R.G., Albanese, C., Reutens , A.T., Segall, J.E., Lee, R.J., and Arnold, A. (1999) The
cyclins and cyclin-dependent kinase inhibitors in hormonal regulation of proliferation and
differentiation. Endocr Rev 20:501-34.
13. Condeelis, J.S., Wyckoff, J., and J. E. Segall (2000) Imaging of cancer invasion and metastasis
using green fluorescent protein. Eur. J. Cancer 36:1671-1680.
14. Condeelis. J.S., Wyckoff, J.B., Bailly, M., Pestell, R., Lawrence,D., Backer, J., and Jeffrey E.
Segall (2001) Lamellipodia in invasion. Sem. Canc. Biol. 11:119-128.
15. Condeelis, J. and J.E. Segall (2003) Intravital imaging of cell movements in tumors. Nat. Rev.
Canc. 3:921-930.
16. Condeelis J, Song X, Backer J, Wyckoff J and JE Segall (2003) Chemotaxis of cancer cells during
invasion and metastasis. In: Cell Motility: from molecules to organisms. (Editors: Anne Ridley,
Michelle Peckham, Peter Clark) Wiley & Sons. 175-188.
17. Kempiak, S.J. and J.E. Segall (2004) Stimulation of cells using EGF-coated magnetic beads.
Sci STKE. 218:pl1.
18. Meili, R., Richard A. Firtel, R.A. and J. E. Segall. (2004) Molecular Mechanisms of Chemotaxis in
Amoebae. In: Chemotaxis (editor Michael Eisenbach) U.K., Imperial College Press. 253-294.
19. Bozzaro, S., Fisher P.R., Loomis, W., Satir, P. and J. E. Segall (2004) Guenther Gerisch and
Dictyostelium, the microbial model for ameboid motility and multicellular morphogenesis. Trends
Cell Biol. 14:585-9.
20. Wyckoff J, Segall JE, and J Condeelis (2005) “Chapter 22: Single cell imaging in animal tumors in
vivo” in Live Cell Imaging: a Laboratory Manual, Eds.: D.L. Spector and R.D. Goldman. CSHL.
Cold Spring Harbor, NY. Pp. 409 - 422.
21. Wang W, Goswami S, Sahai E, Wyckoff JB, Segall JE, Condeelis JS. (2005) Tumor cells caught
in the act of invading: their strategy for enhanced cell motility. Trends Cell Biol. 15:138-45.
22. Ingman, W., Wyckoff J, Xue C, Lin EY, Wang W, Goswami S, Pollard JW, Condeelis J, and JE
Segall (2005) Imaging Invasion and Metastasis In vivo. In: Cell Motility in Cancer Invasion and
Metastasis (editor Alan Wells), Kluwer Academic Publishers.
23. Condeelis J, Singer R, Segall JE (2005) The great escape: When cancer cells hijack the genes for
chemotaxis and motility. Ann. Rev. Cell Dev. Biol. 21: 695-718.
24. Sidani M, Wyckoff J, Xue C, Segall JE, Condeelis J. (2006) Probing the microenvironment of
mammary tumors using multiphoton microscopy. J Mammary Gland Biol Neoplasia. 11:151-63.
25. Wyckoff JB, Condeelis J, Segall JE (2006) Growth factor receptors – doing more than just
stimulating tumor growth? BIF Futura. 21:219-223.
26. Kedrin D, Wyckoff J, Sahai E, Condeelis J, Segall JE (2007) Imaging of tumor cell movement in
vivo. Current Protocols in Cell Biology. 19.7.1 – 19.7.17.
27. Smirnova T, Segall JE (2007) Amoeboid chemotaxis: future challenges and opportunities. Cell
Adhesion and Migration. 1:4, 1-6.
28. Kedrin D, van Rheenen J, Hernandez L, Condeelis J, Segall JE (2007) Cell motility and
cytoskeletal regulation in invasion and metastasis. J. Mam. Gland Biol. Neoplasia. 12: 143-52.
29. Symons M and Segall JE (2009) Rac and Rho driving tumor invasion: who’s at the wheel?
Genome Biology.10:213.1 - .4.
30. Hernandez L, Smirnova T, Wyckoff J, Condeelis J, Segall JE (2009) In vivo assay for tumor cell
invasion. Methods in Molecular Biology. 571:227-38
31. Zhou ZN, Boimel PJ, Segall JE: Tumor-stroma: In vivo assays and intravital imaging to study cell
migration and metastasis. Drug discovery today Disease models 2011, 8(2-3):95-112.
32. Wyckoff J, Gligorijevic B, Entenberg D, Segall J, Condeelis J: The in vivo invasion assay:
preparation and handling of collection needles. Cold Spring Harbor protocols 2011,
33. Wyckoff J, Gligorijevic B, Entenberg D, Segall J, Condeelis J: High-resolution multiphoton imaging
of tumors in vivo. Cold Spring Harbor protocols 2011, 2011(10):1167-1184.
34. Locker J, Segall JE: Breast cancer: the matrix is the message. The American journal of pathology
2011, 178(3):966-968.
JE Segall cv
C. Patents
Application No. 12/998,237.
JE Segall cv
Grant Support
NIH R01CA077522 (Segall, P.I.)
The Roles of ErbB1 and ErbB2 in Metastasis.
Total direct costs $918,000.
04/27/07- 02/28/12
NIH 1 PO1 CA100324-01 (Condeelis, P.I.)
Project 3: ErbB Family and Invasion/Intravasation During Metastasis
(Segall, J.- Project 3 Leader)
Total direct costs $910,000 (Project 3)
Association for International Cancer Research (Segall, P.I.)
1/1/2011 – 12/31/13
Role of an EGF/CSF1 signaling loop in microglial enhancement of glioblastoma invasion
This project will examine the extension of the paracrine invasion loop found with breast cancer invasion
to the invasion of glioblastoma.
Total direct costs $263,848
NIH F31 CA110269 Kirschstein Predoctoral Fellowship (Lorena Hernandez, P.I.) 9/1/07-6/31/10
Characterization of the Invasion and Intravasation of Breast Cancer Cells
Requested Total direct costs $146,166
DOD BC061403 Predoctoral Training Award (Dmitriy Kedrin, P.I.)
The Contribution of ErbB Tyrosine Kinase Receptor Family Members to the Metastatic Progression of
Mammary Adenocarcinomas
Total direct costs $90,000.
NIH 1R21CA125288 (Segall, P.I.)
Development of An In vivo Metastasis Screen
Total Direct Costs: $190,000
Dana Foundation Program in Brain and Immuno-imaging (Segall, JE, PI)
Roles of the EGF Receptor and Microglia in Glioma Invasion.
Total direct costs:$100,000
7/1/06 – 6/20/09
NIH R29 GM44246. Mutational Analysis of Amoeboid Chemotaxis.
$350,000 total direct costs. Principal Investigator: Jeffrey E. Segall
July, 1991 - June, 1996.
American Heart Association NYC Division Investigator Award (salary award). Mutational Analysis of
Amoeboid Motility. July 1991 - Jun 1994. $117,000 total direct costs. Principal Investigator: Jeffrey E.
JE Segall cv
NIH R03 CA54932. Molecular Analysis of Chemotaxis in Metastatic Cells. July, 1991 - June 1993.
Principal Investigator: J. Condeelis. Total direct costs $80,000.
NSF MCB 9304992. Orientation of S. cerevisiae cells in spatial gradients of mating factor. August,
1993 - January 1995. Principal Investigator: J. Segall. Total direct costs $36,166.
US Army Grant 2466. Molecular Analysis of Motility in Metastatic Mammary Adenocarcinoma Cells.
August 1994 - August 1998. Total direct costs $467,152. Principal Investigator: J. Segall.
American Heart Association NYC Division (Established Scientist Salary Award): Molecular Genetic
Analysis of Amoeboid Chemotaxis. July 1995-June 2000. Total direct costs: $216,940. Principal
Investigator: Jeffrey E. Segall
US Army Grant. Tumor Cell Behavior In situ. September 1996 - September 1998. Total direct costs:
$88,205. Principal Investigator: J. Condeelis.
United States-Israel Binational Science Foundation: The Role of Myosin in Chemotaxis of Metastatic
Cells. September, 1997-August, 2000. Total direct costs $130,000. Principal Investigator: S. Ravid
Mortimer Harrison Trust : The Development of an Interdisciplinary Research Program focussed on the
study of breast cancer metastasis and the design of new drugs that inhibit tumor spread. January, 1997December, 1999. Principal Investigator, I. D. Goldman
NSF MCB9728324: Analysis of DdERK2 in Dictyostelium. July, 1998-June, 2001. Total direct costs
$183,834. Principal Investigator: Jeffrey E. Segall
US Army IDEA grant BC980793. Analysis of Metastasis in Transgenic Mice. June, 1999-September,
2002. Total direct costs $210,000. Principal Investigator: Jeffrey E. Segall
US Army IDEA grant BC980718. Isolation of Motile Tumor Cells from Live Breast Tumors. August,
1999-July, 2002. Total direct costs $210,000. Principal Investigator: J. Condeelis
NIH: CA77522R01: The Roles of ErbB1 and ErbB2 in Metastasis. April, 2000 - March 2005. Total
direct costs $900,000. Principal Investigator: Jeffrey E. Segall
NIH: 4R33-CA89829: Novel Imaging Methods for Gene Discovery in Cancer. February 2001 – January
2005. Total direct costs: $1,313,052. Principal Investigator: J. Condeelis.
US Army BC023216 Concept Award(Segall, J.E., P.I.) 9/1/03-8/30/04
Identification of Intravasation Sites Using Magnetic Resonance Imaging
Total direct costs: $75,000.
Breast Cancer Alliance (Segall, PI)
Development of An In vivo Metastasis Screen
Total direct costs $92,000
1/1/07 – 12/31/07
NIH 1R13GM079853 (Segall, P.I.)
2007 Gradient Sensing & Directed Cell Migration GRC
01/01/07 – 12/31/08
JE Segall cv
Total Direct Costs: $13,000
JE Segall cv
Invited non-AECOM Presentations (since 2000)
i. Talks at Meetings and Workshops
Cell Migration and Invasion Keystone Conference, “In vivo Imaging of Tumor
Cell Invasion”, 2001.
Annual Meeting of Japanese Anatomists, “Signal transduction in cancer using
advanced imaging techniques”, 2001.
NCI Metastasis Workshop, “Tumor Cell Migration”, 2001.
Sixth Annual Symposium of the Department of Immunology and Oncology,
Centro Nacional de Biotecnología, Madrid, Spain, “The Signals that Keep Cells
Moving: Migration and Metastais”, 2001
UCSF Workshop on directed cell movement, “Bacterial vs eukaryotic
chemotaxis”, 2002
Sensory Transduction in Microorganisms Gordon Conference, 2002
NCI Glycobiology and Cancer Workshop, “In vivo imaging of tumor cell
metastasis, 2002.
Dynamics of Cellular Organization Keystone Conference, “In vivo Imaging of
Tumor Cell Invasion”, 2003.
Cyto 2003 Astra Zeneca Lecture, “Analysis of metastatic tumor cell behavior”
Beatson International Cancer Conference, “Analysis of metastatic tumor cell
behavior”, 2003
Beatson Internation Cancer Conference Workshop, “ Localized signaling from the
EGF receptor”, 2003
AACR Sunrise session, “ Analysis of cell behavior in metastatic tumors”, 2003
CBCE Second annual symposium on anti-signaling strategies in human neoplasia,
“Imaging therapeutic action in tumors and blood vessels”, 2003
Sensory Transduction in Microorganisms Gordon Research Conference, January
Weizmann-Einstein Joint Symposium, Rehovot May 2004
European Cancer Society Annual Meeting, Innsbruck June 2004
Dynamic Microscopy 2004, Wuerzburg December 2004
Tumor Invasion and Metastasis - Bridging Basic and Clinical Research, Vienna,
March 2005
Systems Integration in Directed Cell Motility, ASCB Summer Meeting, Seattle,
July 2005
Engineering Cell Biology: The Cell In Context, ASCB Summer Meeting, Seattle,
July 2005
Titisee Conference “Mechanisms of Chemotaxis”, Germany, April 2006
Van Andel Research Institute (VARI) Scientific Retreat, Grand Rapids, May 2006
New York Cancer Consortion Developmental Therapeutics Symposium, May
Reproductive Biology Gordon Research Conference, June 2006
Congress of the Croatian Society of Biochemistry and Molecular Biology,
October 2006
Novel Imaging Technologies in Disease Models Workshop, Weizmann Institute,
October 2006
JE Segall cv
Cell migration in development and disease, Paris, November 2006
Orthopedics Research Society Annual Meeting, San Diego, February 2007
3rd International Symposium on Translational Oncology, Barcelona, May 2007
ASCB Annual Meeting Workshop in Intravital Imaging, Chair, December 2008
Gordon Research Conference on Gradient Sensing and Directed Cell Migration,
March 2009
Beatson International Cancer Conference on Motility, Invasion and Metastasis,
Glasgow, 2009
Gordon Research Conference on Gradient Sensing and Directed Cell Migration,
June 2011
ii. Invited Seminars
Brooklyn College, NY, NY March, 2002
University of Pittsburgh, Pittsburgh, PA, March 2002
University of Toronto, Toronto, CA, March 2002
University of Virginia, Charlottesville, VA, April 2002
Louisville University, Kentucky, January 2003
University of Toronto, Toronto, Canada, October 2003
Childrens Memorial Hospital, Chicago, IL, November 2003
Visiting Professor, McGill University, February 2004
Animal Models Initiative, NIH, March 2004
CUNY Queens, May 2005
Beth Israel Deaconess Medical Center, Harvard Medical School, October, 2005
University of West Virginia, November 2005
University of Vermont, November 2005
NYU, December 2005
Van Andel Research Institute Annual Retreat, May 2006
Johns Hopkins University, September 2006
Vanderbilt University, September 2006
Rockefeller University, October 2006
The Feinstein Institute, January 2008
University of Michigan, February 2008
Texas A&M, October 2008
University College London, July 2009
Freidrich Miescher Institute, July 2009
Cleveland Clinic, October 2009
Northwestern University, November 2009
Georgetown University School of Medicine, February 2010
Columbia University, March 2011
iii. Other presentations
JE Segall cv
1. "Cancer, what you must know TODAY" broadcast on "The Today Show" on NBC,
November 18, 1996.
2. Television interview Sixth Annual Symposium of the Department of Immunology and
Oncology, Centro Nacional de Biotecnología, Madrid, Spain, “The Signals that Keep
Cells Moving: Migration and Metastasis”, November 2001
3. Television interview at AECOM with RTVE Spain: March, 2002
JE Segall cv
Other Professional Activities:
Section C below
AECOM College wide Committees
Associate Professors Promotion Committee (1997 – 1999)
LCME Subcommittee IV: Educational Programs (1999 renewal)
Pre-Clerkship Clinical Education (2000 to 2003)
Computer Based Education (2000 – 2003)
MSTP Steering Committee (2000 – present)
Professors Promotion Committee and adhoc reviews (2001- 2002, 2006-present)
Compensation and Tenure Review Committee (2004-2005)
LCME Subcommittee on Information Resources/Library Services (2006)
Neuroscience Faculty Search Committee (2007)
AECOM Senate Benefits Committee (2007)
Secretary for AECOM Senate (2006 – 2008)
AECOM Senate Council (2006-2008)
Biophotonics Center Advisory Committee (2006-present)
Analytical Imaging Facility Advisory Committee (2005-present)
Cancer Center Advances Organizational Committee (2008-present)
Vice Speaker for Einstein Senate (2008-2009)
Speaker for Einstein Senate (2009 – 2012)
Departmental Committees
Faculty Search Committee
Graduate Student Committee
Departmental Publicity Committee
Departmental Senator (2000 – present)
Grant Reviewer for
1. NASA Biological Sciences Review Panel, member, 1995-6
2. Army Breast Cancer Pathobiology study section, member, 1999,2001,2004, 2005
3. NIH ZCA1 SRRB-9(M1) Study Section, 2002
4. NIH CDF-4 Study Section 2002, ad hoc review
5. Army Breast Cancer Translational Research and Concept study sections, 2003-2005
6. P01 Site Visit panel, Charlottesville 2003
7. California Breast Cancer Research Program, 2004, 2005, 2006. 2007
8. National Science Foundation, ad hoc reviewer, various years
9. NIH ZRG1 Fellowship Study section, 2005
10. Member of site visit team for Canadian Breast Cancer Research Alliance (CBCRA
research grant competition in the area of "New Approaches to Metastatic Disease"
11. NCI Small Animal Imaging Resource Program Special Emphasis Panel, 2006
12. American Cancer Society Study Sections, 2006 – 2008, 2012
JE Segall cv
13. Ad hoc member of 2007 DOD Breast Cancer Research Program Integration Panel
14. NCI Tumor Microenvironment Study Section: Ad hoc, 2007/2008, permanent
member, 2009 .
Reviewer for
1. AACR Annual Meeting Program Committee
2. American Journal of Physiology
3. Biophysical Journal
4. Biotechniques
5. Breast Cancer Research and Treatment
6. Cancer Cell
7. Cancer Research
8. Cell Motility and the Cytoskeleton
9. Clinical Cancer Research
10. Current Biology
11. Experimental Cell Research
12. European Journal of Cell Biology
13. International Journal of Cancer
14. Journal of American Chemical Society
15. Journal of Biological Chemistry
16. Journal of Cell Biology
17. Journal of Cell Science
18. Journal of Eukaryotic Microbiology
19. Journal of Microscopy
20. Journal of Neuroscience
21. Journal of Proteomic Research
22. Nature
23. Nature Reviews Cancer
24. Molecular Cancer Research
25. Molecular Cancer Therapeutics
26. Oncogene
27. Proceedings of the National Academy of Sciences
28. Science
29. Trends in Cell Biology
Meetings attended (last 5 years):
see invited presentations
JE Segall cv
C. Teaching and Related Activities
Histology: Previously course director. Currently 2 lectures (fall course) and 1 lecture (summer
Cancer: A Basic Science Approach: - Co-director. 3 lectures.
Molecular Cell Biology: - 2 lectures
Mechanisms of Disease – 1 lecture
Responsible Conduct of Research – 1 lecture, 1 small group discussion
Personal Statement
Teaching is a critical part of the academic life of the College. For both medical students as well
as graduate students, the teaching that I perform is focused on developing self-sufficiency in terms of
analysis and understanding of the material that is taught. In the cancer biology course, we try to
inspire an interactive discussion as part of the lecture, as well as requiring that students present and
evaluate critically important cancer research. For students and postdoctoral fellows in my research
group, I try to emphasize independence and acquisition of new skills as a way of preparing for a
career in research.
1. Courses for which I have primary responsibility
Cancer: A Basic Science Approach:
Charles Rogler and I initiated this course in 1995 and coteach it every other year. We
decide on the textbook, lecture topics and organize lecturers for the topics. In addition to attending
lectures (18 90 minute lectures), students are required to do homework sets and discuss a cancer related
paper from the literature during the course. I gave 3 lectures, on growth factors and cancer, cell adhesion
and angiogenesis, and tumor progression and metastasis. We write and grade the homework problems as
well as help in selection of papers to be presented. Students are graded based upon performance in class,
on the homework sets and on the presentation.
Other teaching activities.
Histology – I previously directed the Histology course and now give 2 lectures in the course.
Cell Design Principles: - I give 2 90 min lectures and write questions on the lecture topics for the
final exam.
3. Graduate Students
Names of current students in laboratory
1. Zhenni Zhou (since 2009)
JE Segall cv
2. Sangeeta Jayakar (since 2010)
3. Lizandra Jimenez (since 2010)
4. Ramon Cabrera (since 2011)
Graduated students
Yiwen Wang (Ph.D., 1998)
Stephan Kempiak (Ph.D. 2004)
Songyang Chen (Ph.D. 2006)
Dmitriy Kedrin (Ph.D. defense 2008, awarded after MD 2010)
Lorena Hernandez-Teller ( Ph.D. defense 2010, will be awarded after MD 2012)
Tatiana Smirnova (Ph.D. 2010)
Pamela Boimel (Ph.D. defense 2011, will be awarded after MD 2013)
Rotational Students
1. Susan Masseling (1994)
2. Amanda Chan (1995)
3. Francine Garrett (1998)
4. Dmitriy Kedrin (2003)
5. Lorena Hernandez (2004)
6. Tatiana Smirnova (2005)
7. Pamela Boimel (2006)
8. Adina Buxbaum (2008)
9. Zhenni Zhou (2008)
10. Natasha Chandiramani (2011)
Advisory Committees:
1. Dianne Cox (1990 - 1995)
2. Robert Eddy (1990 – 1996)
3. Angela Rene Taylor ( 1994 – 1996)
4. Cynthia Isaac (1995 – 2000)
5. Amanda Chan (1996 – 1999)
6. Vaughn Latham (1996 – 1999)
7. Ilia Ichetovkin (1998 – 2000)
8. Jeffrey Levsky (1998 – 2002)
9. Qionqing Wang (1998 – 2002)
10. Ghassan Mouneimne (2001-2005)
11. Nupur Kittur (2002 - 2006)
12. Christian Faul (2002 – 2004)
13. Xiaoyan Song (2002 – 2006)
14. Aashir Awan (2002 – 2005)
15. Yu Liu (2002 – 2003)
16. Aaron Bell (2003 – 2006)
17. Mazen Sidani (2003- 2007)
18. Wassim Abou-Kheir (2004 – 2007)
19. Corina Sarmiento (2004 – 2007)
20. Mirvat El-Sibai (2004 – 2007)
JE Segall cv
21. Laureen Ojalvo (2004 – 2008)
22. Salvatore Coniglio (2005 – 2007)
23. Reniqua House (2005 – present)
24. Pei-wen Chen (2005- 2007)
25. Alfred Adamako (2006 – present)
26. Matthew Oser (2006- present)
27. Marianella Arias (2007 – present)
28. Christina Medina (2007 – present)
29. Ying Xiong (2007 – present)
30. Dan Ishihara (2007 – present)
31. Zachary Katz (2008 – present)
32. Yael Barach (2009 - present)
Qualifying Examinations:
1. Robert Eddy (1993)
2. Diane Cox (1992)
3. Angela Taylor (1996 & 1997)
4. Cynthia Isaac (1996)
5. Yan Fan Chan (1997)
6. Sergei Levin (1998)
7. Aashir Awan (1999)
8. Qiongquing Wang (1999)
9. Ilia Ichetovkin (1999)
10. Dahlene Fusco (2000)
11. Jeffrey Levsky (2000)
12. Xiaochua Gong (2000)
13. Xiaoyan Song (2002)
14. Eustratios Bananis (2003)
15. Aaron Bell (2003)
16. Sal Coniglio (2004)
17. Mazen Sidani (2004)
18. Wassim Abou Kheir (2005)
19. Reniqua House (2006)
20. Su Chung (2006)
21. Roberta Kiffin (2007)
22. Subjahit Mukherjee (2007)
23. Matthew Oser (2007)
24. Jelena Pribic (Hunter College, 2007)
25. Maria Kon (2008)
26. Leora Nusblat (2008)
27. Zachary Katz (2009)
28. Sami Hocine (2009)
29. Adina Buxbaum (2009)
Ph. D. Thesis Defense:
Anne Bresnik (1992)
Dianne Cox (1995)
Robert Eddy (1996)
Yan Fan Chan (1999)
Vaughan Latham (1999)
Wenjie Luo (2000)
JE Segall cv
7. Cynthia Isaac (2000)
8. Ilia Ichetovkin (2002)
9. Jeffrey Levsky (2002)
10. Qiongquing Wang (2002)
11. Sergei Levin (2003)
12. Chen Wang (2004)
13. Christian Faul (2005)
14. Mirvat El-Sibai (2007)
15. Corina Sarmiento (2007)
16. Wassim Abou-Kheir (2007)
17. Pei-wen Chen (2007)
18. Rosanna Pezo (2007)
19. Kyle Lapidus (2007)
20. Salvatore Coniglio (2007)
21. Yu Liu (2007)
22. Maria-Berenice Duran (Hunter College, 2007)
23. Mazen Sidani (2008)
24. Binzhi Zhu (2009)
4. Postdoctoral Students
Names of current postdoctoral students in laboratory
1. Salvatore Coniglio
2. Uttama Rath
Graduated students
Sangeeta Tyerech (MD research rotation, 1995)
Ross Hammerman (MD research rotation, 1998)
Maryse Bailly (1998-2000)
Fayyaz Ahmed (1999-2002)
Stefania Violini (2003-2005)
Chengsen Xue (2002-2006)
James Hulit (2008 – 2009)
5. Summer Students
Joey Liu (1992)
Herbert Lee (1993)
Nouri Zalta (1995)
Lauren Insel (1997)
Sandra Bravo (1998)
Vonetta Sylvestre (1999)
Kun-Lin Tsai (Tony) (2001)
Shlomo Koyfman(2003)
Melanie Lee (2004)
Rachel Schnur (2005)
Daniel Kimm (2006)
Rachel Yamnik (2007)
Kira Lawton (2008)
Tirtza Spiegel (2009)
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