Course Number: Palm Beach State College General Class and Course Information

by user








Course Number: Palm Beach State College General Class and Course Information
Palm Beach State College
Course Syllabus – Classroom – Internet Component Class
General Class and Course Information
Course Number: BSC 2421L Course Title: Intro. to Biotechnology Laboratory
Term/Dates: Fall 2016 (2017-1)
Reference Number: 217110
Credit Hours: 2
Days: Tuesdays & Thursdays
Time: 2PM- 4:45PM
Room: SC 214
Course Description: This laboratory course provides hands on experience for basic and common biotechnology
laboratory techniques in the areas of laboratory safety, aseptic techniques, measurements, calculations, preparation
of solutions, use of pH meters, spectrophotometers, centrifuges, etc., as well as training in specific biotechnology
techniques, including DNA extraction and amplification, plasmid transformation, agarose gel electrophoresis,
preparation of LB broth and agar plates and restriction digestion of DNA. In a lecture science course where there is a
required co-requisite lab, students may withdraw from the lab class, but stay in lecture class. (Students may also
choose to withdraw from both.) Students will not be allowed to withdraw from lecture and remain enrolled in lab.
Component Class Information
This course is supported by an Internet course website. Some of the course material is available online and requires
you to have Internet access. Before you decide to take the course, it is recommended that you:
Visit – www.palmbeachstate.edu/elearning/online
to obtain Detailed Facts & Information about
Blackboard Access
 Access to your Online Course is AVAILABLE each term on the FIRST DAY OF CLASSES
 Access to your Online Course will NOT BE AVAILABLE unless the course tuition is FULLY PAID
Blackboard Orientations
 How to Register for the Free Online Orientation Course: ELO1000
 Where/When to Attend On-Campus Orientation
Blackboard Tips & Tutorials
 How to Ensure your Computer is Compatible
 Ensure you Understand Examination and Assignment Rules
 Learn the Functions of Blackboard
Then Proceed To – https://palmbeachstate.blackboard.com
to Login to your Course (not available before the first day of class) using the same
USERNAME and PASSWORD you used to register for classes.
For Additional Information and Notices Visit – http://www.palmbeachstate.edu/elearning
The free Blackboard Learn Mobile App can be downloaded to your phone/tablet for access to Bb course content. It also provides pop-up notifications when announcements and
grades are posted.
Professor’s Information
Name: Becky Mercer, Ph.D.
Office Location: SC 205 (inside Office Suite SC 204)
Telephone: 561-207-5059
Email: [email protected]
Faculty Web Page:
Office Hours: By Appointment
Information on REQUIRED Textbooks:
Template Date - July 2013
Palm Beach State College
Course Syllabus – Classroom – Internet Component Class
Biotechnology: Science for the New
Millennium Laboratory Manual w/CD
Revised 1st
Spiral-bound Carbon-Copy Laboratory
Notebook (barbakam.com)
100 page
Copy-Page n/a
(Green cover w/yellow spiral binding)
Suggested Supplementary Books:
Basic Laboratory Calculations for
REQUIRED Materials:
Safety Items
Lab Coat (student supplies own lab coat)
Liquid-Safe Vented Lab Goggles (student
supplies own goggles)
Also acceptable: “Visorgogs” #1 Model
referred to as “Clearview” and
suggested for general use
Template Date - July 2013
Daugherty E.
Edition Author
Seidman L.A.
Trench coat length
with full-length
Publishing Inc.
Education, Inc.
publishing as
Special Note
Can Be Purchased at
PBSC Bookstore; Booksmart;
uniform shop; etc
Must be vented
goggles or
“visorgog” style;
NOT safety
PBSC bookstore; Booksmart;
uniform shop; Home
Depot/Lowes; etc
Visorgogs produced by: Jones
and Company in Providence
Palm Beach State College
Course Syllabus – Classroom – Internet Component Class
Other Information on Textbooks and Materials needed for class:
This course has a combination of a required textbook and separate pdf format protocols. All experiment protocols and
necessary files/information can be found on the Blackboard course website.
Please Note: Some students find that they need to purchase a second notebook because they fill the first one.
Barbakam.com offers the notebook in both a 50 page and 100 page versions.
Other Required Materials:
• Calculator (any type: standard, scientific, etc.), Black pen, Ruler, a LOT of clear tape
• Access to a computer containing Microsoft Office with connection to a printer
• Separate Binder or notebook for storing hand-outs
• Digital camera / smart phone for taking pictures (not allowed as a calculator for exams/quizzes)
• Sharpie black ultra fine tip marker (for labeling your tubes)
Course Learning Outcomes: As a result of taking this course, the student will be able to:
• Identify and apply the common safety precautions in a biotechnology laboratory.
• Recognize and employ standard aseptic and sterility techniques.
• Calculate and measure different compounds and reagents based on the metric system.
• Prepare normal and molar, weight by volume, volume by volume, and percentage solutions, as well as different
buffers, and be able to operate pH meters and spectrophotometers.
• Handle and prepare bacterial cultures and media.
• Perform DNA extraction, quantification and analysis.
• Perform bacterial transformation and antibiotic selection for recombinant bacteria.
• Perform polymerize chain reaction (PCR).
Full Course Outline: http://www.palmbeachstate.edu/asp/IE_ShowOutline.asp?show=Y&CourseID=BSC2421L
Full Course Outline - Click on the following link: Course Outlines and enter the course number (no space between the
prefix and the number).
Class Information
Co-requisites: BSC 2421 (with a grade of C or higher)
Recommended Prerequisites: BSC1010, BSC1010L, CHM1045, CHM1045L (with a grade of C or higher)
Class Schedule: Class will meet on Tuesdays & Thursdays from 2PM – 4:45PM in SC214. Lab meets twice a week for
2hrs and 45 minutes each time. Students are required to attend every session. Students are required to take all
quizzes/exams related to the course and participate in all assignments. Students are required to come to class prepared
for the lab experiment that will be conducted that day, and with their pre-labs completed. Students are expected to
turn-in lab reports on the days they are due.
Academic Dishonesty Policy and Penalty for Violation:
Any suspected act of academic dishonesty or cheating, either active or passive, will result in a grade of “F” for the
course. If the suspected act of cheating occurred after the deadline to withdraw then the grade of “F” will be issued for
the course and you will be removed from the course permanently. If the suspected act of cheating occurs before the
“drop/add deadline” then the student will be asked to immediately withdraw from the course. The faculty member
reserves the right to bring the issue before the Associate Dean at his or her discretion and to refuse to allow the student
to participate in any of the Instructor's future courses. The Dean of Student Services may also be notified and further
action by the college may be taken against you and anyone else associated with the incident(s). Please be advised that if
you are a student within the biotechnology program and undergo disciplinary action by a professor for academic
Template Date – July 2013
Palm Beach State College
Course Syllabus – Classroom – Internet Component Class
dishonesty, you may forfeit your opportunity for an internship position. Ignorance of the rules is no excuse for your
actions. 100% Academic honesty and integrity will be expected from you.
Assignments, Tests, Quizzes and Final Exam Schedule, with percentage/points of grade:
The grading scale is based upon attendance, participation, lab reports, and exams. This means that participation and
attendance makes a significant portion of the final grade. An accurate record of attendance will be kept for each course.
If a student misses one-third or more of a class session (or “disappears” for long periods of time) the student will be
counted absent. Three tardies will count as one absence. Leaving early is the same as being tardy. If a student is absent
on the day of an exam/quiz/assignment/experiment, they will receive a grade of zero. It is the student’s responsibility to
ensure all assignments are turned in on time. If you know that you will miss a future class period it is your responsibility
to ensure that any work due that day is delivered to the professor by the start of class. Due to the number of lab reports
that will be generated, the total number of possible points is variable from semester-to-semester, so the grade
breakdown is given in overall percentages.
Exam Formats
- Format is mostly paper short answer & problem-based; very few MC or T/F. No Scantron.
- Midterm exam may have a Practical (Hands-on) portion in addition to the written portion.
- Any assistance can be provided via Supplemental Instructor, email, course website, or your own study
*Tentative* Exam & Event Schedule
All quizzes and exams will take place during the on-campus Laboratory Session in room SC 214.
• Midterm Exam: Tuesday, October 18 at 2:30 pm – 4:00 pm. Room SC 214.
• Tentative Field Trip Date: Tuesday, November 22nd at 2:00 pm – 4:45 pm. Location: Scripps Florida.
• Poster Session: Monday, December 5th 1:00 pm – 3:00 pm. Room SC 127.
• Final Exam: Tuesday, December 6th at 2:30 pm – 4:00 pm. Room SC 214.
• Quizzes every Thursday
o Lab Reports; each report is worth 20 points (1 lowest will be dropped):
40% total
o Quizzes & Homework (2 lowest quizzes, 1 lowest Homework will be dropped):
10% total
o Participation and Attendance
10% total
o Midterm & Final Exam; each exam is worth 100 points:
40% total
Students should make note of the importance of lab reports to your final course grade (40%). Because this
is a LABORATORY COURSE, the lab reports weigh heavily on your course grade.
Letter Grade
Template Date – July 2013
Percentage (if applicable)
≤ 59
Palm Beach State College
Course Syllabus – Classroom – Internet Component Class
Class Policies
Attendance: Professors are required to take attendance.
Attendance to the first day of class is Mandatory. Failure to do so will result in the student being dropped from the
course. Students must sign the attendance log at the start of every class. Failure to sign in will result in a recorded
absence, even if the student was actually in class. You must sign in to receive credit for being in class. No exceptions.
Students will be expected to obtain all necessary lab materials from the Blackboard course website and participate in
course discussions on the Blackboard website. Students are required to access the course website at least two times per
week to read the required lab materials, check the discussion board and course email regularly. Students are expected
to attend ALL class-related sections/events. 100% attendance and participation is expected. Students are required to
sign-in on the attendance sheet immediately at the start of class-time. Failure to do-so may result in you being
considered absent for the day.
Electronic Device Use and Email Policy:
The use of cell phones or other electronic devices during class is prohibited. If a student is caught using any such device,
they will be asked to leave. Cell phone cameras and calculators are allowed for use for lab experiment purposes only; no
personal use (i.e. non-class related use) will be permitted. No cell phones will be allowed during exams or quizzes.
Late Assignment Policy:
All required assignments are expected to be completed on time. LATE ASSIGNMENTS WILL NOT BE ACCEPTED. If, due to
an emergency, a student cannot meet the due date, s/he is to provide evidence to AND request an extension from the
professor. The professor reserves the right to deny or grant any request. Documented evidence from a
doctor/emergency room etc will be necessary for extension requests. Assignments turned in late will have significant
point value deducted from them (minimum of 10%). It is at the Professor’s discretion to either accept or deny a late
assignment. All lab reports are due immediately at the start of class; failing to turn-in a lab report at class start time may
result in the professor deducting points or granting a grade of zero for the assignment.
Make-up Exam Policy:
Make up tests/quizzes are only given in emergency situations. These exams may be in a different format than the
original class exam. The student is responsible for the fee to take the proctored test in the Test Center. Special
arrangements for make-up exams should be discussed with and approved by the professor. For any other situation, a
grade of zero will be given for missed exams/quizzes. There are absolutely NO make-up lab experiments.
Withdrawal Policy:
Students who fail to comply with the initial course requirements (i.e. attending the first day of class and logging into the
Blackboard course website by midnight of the first day of class) will be required to drop/withdraw from the course. After
the first day of class, any student finding themselves in a situation where they are required or chose to withdraw from
the course should notify the instructor and also follow the prescribed college procedures for withdrawing from a course.
Students should be mindful of the final date to withdraw for each term. Any student failing to properly withdraw from a
course will receive a final (possibly failing) course grade issued by their instructor.
Email Policy: The blackboard e-mail system is the official electronic communication from the Professor to all the
students registered in this course, and from all the students to the Professor. The students are considered officially
informed of any changes or special instructions or announcements through this system. The instructor's Blackboard email address and discussion board will be checked once per day Monday to Thursday from 9:00 am – 6:00 pm. This
means that weekends and holidays are not officially check days. The instructor will do their best to respond to all
emails within 24 – 48 hrs, however, response time may vary due to holidays/weekends.
Template Date – July 2013
Palm Beach State College
Course Syllabus – Classroom – Internet Component Class
Professor’s Expectations: The student is expected to participate in 100% of the lectures and laboratories along with
completion of all assignments, quizzes, and exams on-time. Students are expected to conduct themselves in a mature,
respectful manner at all times.
Methods of Instruction: Online web-component for distribution of laboratory materials along with communication
and on-campus laboratory experiments. In-class laboratory experiments will be performed during the scheduled class
Unique Requirements of the Class: Following the online content of lab material, plus attendance and participation of
the weekly lab sessions on campus, plus all exams/quizzes/assignments/course-related material.
Ensuring Success
- Read and Understand this Syllabus
- Perfect attendance
- Completion of exercises/reading assigned to complete before class;
- Asking questions one of the most important skills you can learn in a college course. (Not the ones you may
have to ask because you were not paying attention or didn't do the reading.)
• Ask if I use a word you are not familiar with
• Ask for me to explain why, or how, or when etc. when needed
• Ask when you want a deeper understanding of a concept
- Use text book, library, provided CD-ROM, and internet extensively
- Ask for help if you are unsure about anything; many things in the lab are more expensive than you would
believe; shyness is not an excuse
- Because of the nature of the course, there is virtually no way that everything you need to know can be
written into class notes - pay attention and add extra notes of your own when necessary. (I will not test
this extra-knowledge, though – it’s just that, if you are serious about this career, you have to be able to
‘pick up’ on bits of trivia.)
Classroom Environment
- Clean up after yourself-failure to do so will result in deduction of points from lab reports
- Behaviors which will result in grade reduction:
• "Disappearing" from class for an extended time
• Not keeping/leaving a clean work space
• Negligence and/or abuse of equipment
• Questions or actions that indicate you are not prepared
• Non-participation
• Disruptiveness (interfering with teaching and learning)
• Academic dishonesty
• Disrespectful behavior towards classmates/professor
Laboratory Safety Guidelines
1. Laboratory Manual (Daugherty) – You MUST read and familiarize yourself with the “Background” sections on pp.
2. You MUST be attentive to any additional guidelines and warnings given by the instructor.
Laboratory Notebook – Basic Guidelines for Legal Scientific Notebook
1. Use only ink; no pencil
2. Use a spiral-bound carbon copy notebook (press firmly because carbon copy doesn’t always transfer well)
3. All pages numbered in ink before use (on upper, outside corner of page)
4. Page 1 for identification information
5. Pages 2-4 for Table of Contents (unless a ToC page is provided in the notebook)
6. When filling only part of a page, you may cross through the empty space
7. NEVER remove non-perforated pages-under any circumstance
Template Date – July 2013
Palm Beach State College
Course Syllabus – Classroom – Internet Component Class
8. When photos or computer printouts must be inserted, tape them in on all sides and sign/date over the
edge-NEVER staple anything into the notebook.
9. Do not use abbreviations, names or symbols that other people will not understand
10. Be honest – even mistakes must be recorded.
11. NEVER use “white-out” correction fluid or “blacking out”; incorrect entries may be lined out with a single
line only and must be labeled with initials and the date. NEVER cover-up data in the notebook.
Correct: Mistakes bm 7/8/16
Incorrect: Mistakes
12. Write data generated during the experiment directly into notebook. Do NOT keep scrap papers that can be
misplaced or jumbled out of order. There are NO ROUGH DRAFTS. All data must be entered directly into the
lab notebook at the time of the experiment.
13. “Your” notebook is typically the property of your employer; in a workplace setting it should never be taken
home. Expect others to read it and therefore write your reports as though they are directions for someone
14. Each day, sign and date at the bottom of each page that you use. Have a “witness” sign and date as well.
• This IS done in industry. Your witness can attest you performed the experiment on the days you specify.
15. Under no circumstance is the witness to sign before you do.
16. For each experiment/project, your notebook entries should include: Title, Purpose, Materials, Protocol,
Data/Results, Conclusions, and References (where appropriate). More details will be given in class.
17. For each experiment, you are required to have a PRE-LAB completed before you come to class that day. The
pre-lab consists of Title, Purpose, Background, Materials, and Protocol. The pre-lab is worth 5 points out of
the 20 total points for your Lab Report. Failure to have the pre-lab complete will result in deduction of points
from lab report grade. Leave room for notes/changes/calculations. Also leave room for the Data/Results,
18. Lab reports are 40% of your course grade. They are important! Each (20 points each) must contain the
following sections:
Title: At the top of the page.
Purpose: A “To” statement explaining the goal of the experiment. “To isolate DNA from strawberries.”
Background: 1-2 paragraphs explaining the background of the subject and/or technique being performed. Can be
directly from the lab manual.
Materials: List of ALL reagents, all equipment, all instruments and all supplies, used for the experiment on that
particular day only. Each new day requires a different Materials section with a complete list of all reagents,
equipment, supplies used that day only. Materials requires listing the VENDOR, CATALOG Number, and LOT
(batch) number, and expiration date, where appropriate.
Protocol: Itemized (ie numbered) steps performed (in order) with details. Leave room for any changes or
calculations that may occur on the day of the lab. Written so that someone who wasn’t there can repeat the lab
following your written instructions in the protocol. May include tables /charts of reaction setup instructions. Each
new day requires a different Protocol section. At the end of a day, write, “END DAY 1” or END DAY 2” etc. Include
all steps, including Wash hands, get ice, pre-warm water bath to 65C, etc.
Data/Results: Labeled and numbered figures, photos, and tables each containing an objective description of your
findings (Fig 1. Photo of bacterial colonies growing on Amp agar plate (plate described in detail); Fig 2. Photo of
agarose gel containing DNA samples under UV light (list all samples in each lane); Table 1. Table of class
NanoDrop readings, showing purity and yield of each student’s genomic DNA and class average. Fig 3. Graph and
NanoDrop report of my (student) DNA. Where available, include data from the class as a whole, the class average,
along with your own results for comparison.
Conclusions: Answer these 3 questions for the Conclusions section of each lab report.
a. Explain the results of your experiment (1-2 paragraphs). Include an explanation of how your result(s)
compare to others in the class, and whether you got the result(s) you expected.
Template Date – July 2013
Palm Beach State College
Course Syllabus – Classroom – Internet Component Class
b. Describe what technical details you would change about this experiment to improve your result and why
this would improve your data (3-5 sentences minimum).
c. Describe 1 or 2 real-world applications of this technology and/or protocol in the biomedical, agricultural,
marine biology, or pharmaceutical world (2-3 sentences minimum).
H. References, if used.
College Policies and Web Information
Academic Dishonesty
Academic dishonesty includes the following actions, as well as other similar conduct aimed at making false representation with
respect to the student's academic performance:
(1) Cheating on an exam, (2) Collaborating with others on work to be presented, if contrary to the stated rules of the course,
(3) Submitting, if contrary to the rules of the course, work previously submitted in another course, (4) Knowingly and intentionally
assisting another student in any of the above actions, including assistance in an arrangement whereby work, classroom
performance, examination, or other activity is submitted or performed by a person other that the student under whose name the
work is submitted or performed, (5) Plagiarism.
Please refer to the Palm Beach State College Student Handbook (www.palmbeachstate.edu/current for link to the student
Classroom Etiquette and Student Behavior Guidelines
Students will demonstrate respect for professors and fellow students. Behavior that is disruptive to a positive learning environment
reported by the professor will result in a warning on the first instance; the second instance might result in expulsion from the course
or campus.
Computer Competency Component
Each student will, to the satisfaction of the professor, demonstrate a fundamental understanding of basic computer operations
through various professor-determined exercises and/or assignments.
Disability Support Services
Students with disabilities are advised, in compliance with federal and state laws, that accommodations and services are available
through the office of Disability Support Services (DSS). It is the student's responsibility to contact Disabled Student Services Advisors
and to submit appropriate documentation prior to receiving services. Please see the website at www.palmbeachstate.edu/current
for web address for Disability Support Services.
Eating, Drinking and Smoking
Eating and drinking are confined to areas designated on the campus. Smoking is not permitted in any College building and only in
areas designated at each campus.
Final Course Grade Appeal
The evaluation of academic work is the responsibility of the faculty member/instructor. The method for assigning the final course
grade is established by the faculty member/instructor. Per Board Rule 6Hx-18-3.191, faculty/instructors shall communicate the
grading policy of the course to their students in writing. If this policy changes during the term, students should be notified of any
changes in writing. The method to determine final course grades must be given to students in a course syllabus at the beginning of
the class. Should a student have a question about a final course grade, please follow the procedures as outlined in the student
handbook: www.palmbeachstate.edu/current.
Mid-Term Grade Reporting
Within one week after the close of the mid-term grading period, mid-term grades will be made available to all students for each
class by either written notification or individual conference.
Student Responsibility Policy
When a student attends the College, s/he becomes subject to its jurisdiction. Students are expected to conduct themselves in a
responsible manner, in all areas of campus life. By enrolling, they pledge to obey the rules and regulations of the College and are
responsible for observing all College policies and procedures as published in the student handbook, the College catalog and other
College publications. The student will be responsible for preparing for class, participating in class, and completing assignments on
Template Date – July 2013
Palm Beach State College
Course Syllabus – Classroom – Internet Component Class
Palm Beach State Websites of Interest
Please see this web page (www.palmbeachstate.edu/current) for a list of web addresses for students.
Withdrawal Policy for Individual Courses
The last day to withdraw from a College course with a "W" grade in this course is November 4, 2016 It is the responsibility of the
student to use the PantherWeb system or visit a campus Registrar’s office to withdraw. An official withdrawal entitles the student
to a grade of "W" in the class.
Other Information from your Professor:
*Tentative* Syllabus (Subject to Change)
Lab / Activity
The lab protocols in this course are taken directly from the
“Biotechnology Laboratory Manual” by Daugherty).
These protocols are also found on Blackboard (Bb).
Introduction to Laboratory. Policies on grading, and attendance
will be provided. Safety rules including waste disposal, lab-coats,
safety glasses, eye-wash, and shower.
Discuss Pre-Labs and Lab Report Format
Practice Lab Activity: Strawberry DNA Isolation: Discuss lab
report preparation & “Pre-labs”
Biology/Chemistry/Biotech Assessment/Quiz (not graded)
Ex #1: Aseptic Technique. This lab will show you about working
aseptically. It is easy to contaminate reagents and your work area.
Finish Ex #1. Take photos of plates (=Data); record observations
in lab notebook. Write Conclusions at home.
Discuss Lab Report Requirements.
Ex#2 (3a): Pipet Use: Measuring Small Volumes (3a). Compare
your tubes a-d absorbance & color with key.
T 9/6
Ex#3 (3b): Pipet Use: Measuring Very Small Volumes (3b)
Quiz #1
Ex# 4 (3c): Using Balances: Measuring Mass (3c) AND
Ex# 5 (3d): Checking Accuracy of Micropipets Using a Balance
Finish Ex Ex#5 (3d). Discuss Homework.
Quiz #2
Ex #6 (3e): Solutions and Spectrophotometer
This experiment runs long.
Ex#7 (3f): Do 3f (Part III gelatin step, p51) first because it takes a
long time for gelatin to dissolve.
Quiz #3
Ex #8 (3g): Making Solutions of Differing Molarity
Ex#9 (3h): Making Dilutions of Concentrated Solutions (3h)
Quiz #4
Ex #10 (7c): Measuring the pH of Solutions (7c).
Template Date – July 2013
What is DUE:
*Your pre-lab is always due at the start of class.
*TLAB = Thinking Like A Biotechnician
Due at the start of Lab:
Sign in, find your seat, introduce yourself.
Pay attention. No cell phones please.
Read pp 1-5 on Lab Notebook & Safety (we will NOT do the cheese lab).
Due at start of lab:
Pre-lab for Strawberry DNA Activity.
Have read and printed “Lab Practice Activity (Strawberry DNA)”
Have read Biology & Chemistry review (Bb).
Due at start of lab:
Lab Report for Strawberry DNA Activity (Practice)
Aseptic Technique pre-lab (see 1 page protocol on Bb). NOTE: use
saliva as (+) control, NOT bread mold.
Due at start of lab:
Lab Math handout to be given in class. Work on this at home.
Due at start of lab:
Lab Report for Ex#1
Pre-lab for Ex#2 (3a, p. 32-35).
Due at start of lab:
Lab Reports for Ex#2 (3a)
Pre-lab for 3b (p. 35)
Due at start of lab:
Lab Reports for Ex#3 (3b)
Pre-lab for Ex#4 (3c, p 40-41)
Pre-lab for Ex#5 (3d, p. 43-44)
Due at start of lab:
Lab Report for Ex#4 (3c)
Lab Report for Ex#5 (3d)
HW#1: TLAB Questions (1-4, p42) & Conversion Review Sheet (p
Have read: pp.45-48 about Solutions
Due at start of lab:
Pre-lab for Ex#6 (3e, P. 45-49)
Have Read “Using the Spectrophotometer to Check CuSO4.5H2O
Samples” (P47).
Due at start of lab:
Lab Report for Ex#6 (3e).
Pre-lab for Ex#7 (3f, P. 50-54)
HW#2: Review Sheet Page 54.
Due at the start of lab:
Pre-lab for Ex#8 (3g) (p. 55-57)
Lab Report for Ex#7 (3f).
Due at the start of lab:
Lab Report for Ex #8 (3g).
Pre-lab for Ex#9 (3h, p59-61).
Due at the beginning of lab:
Lab Report for Ex #9 (3h).
HW#3: Review Sheet Page 62.
Pre-lab for Ex#10: 7c (P. 134-136)
Palm Beach State College
Course Syllabus – Classroom – Internet Component Class
Lab / Activity
The lab protocols in this course are taken directly from the
“Biotechnology Laboratory Manual” by Daugherty).
These protocols are also found on Blackboard (Bb).
Ex #11 (Strawberry DNA Handout): Isolate strawberry DNA
again and read the absorbance using the NanoDrop. Save DNA in
a labeled Eppendorf tube in TE at 4C!
Ex #11 (NanoDrop): Use the NanoDrop “Nucleic Acids” to
determine the concentration and purity of the DNA for gel loading.
Quiz #5
Finish Ex# 11 if needed.
Ex #12 (4i): Making Agarose Gels for Separating and Analyzing
DNA Fragments. Proper preparation of Agarose gels is an
essential skill for scientists to learn.
Ex 12 (4j): Using Agarose Gel Electrophoresis to Study DNA
Molecules. You will load your strawberry DNA from Ex#11 into
your agarose gels.
Discuss how to determine volume and mass of DNA (how many
nanograms of your strawberry DNA are in 20uL? You will need
this skill for the next lab).
Ex #13 (4h): Genomic DNA Extraction from bacteria (4h). P. 7982. Use NanoDrop to determine concentration & purity. Prepare
agarose gels and load bacterial DNA.
Repeat making of Agarose Gels (4i) P. 82-87.
What is DUE:
*Your pre-lab is always due at the start of class.
*TLAB = Thinking Like A Biotechnician
Due at the beginning of lab:
Lab Report for Ex#10 (7c)
Pre-lab for Ex #11 (see Bb handout on NanoDrop)
Have read p 146-150 (Spec readings for DNA analysis).
Due at the beginning of lab:
Lab Report for Ex #11 (if experiment was completed last lab).
Pre-lab for Ex 12 (4i & 4j, P82-84 & P85-87): Note changes to
We will NOT use salmon or yeast DNA.
Make sure to set a water bath to 65C
Step 1 (P83). Make 50mL (not 100mL) of agarose.
Step 11 (P84): Add 1uL of Ethidium bromide stock
(10mg/ml) to your gel once it has cooled to ~65C.
Well 1 (Vf = 15uL): Load 15uL 1X DNA Loading dye (dilute 1:10 in
Well 2 (Vf = 15uL): Load 15uL Lambda DNA cut with HindIII
enzyme (= ladder)
Well 3 (Vf = 10uL): Load 10uL O’GeneRuler DNA ladder (ready to
Well 4 (Vf = 10uL): Load 10uL Bio-Rad 1kb Molecular Ruler (DNA
Well 5 (Vf = 20uL): Premix 5uL strawberry DNA (Ex #12) + 2uL 10X
Loading dye + 13uL dH2O.
Well 6 (Vf = 20uL): Premix 10uL strawberry DNA (Ex #8) + 2uL 10X
Loading dye + 8uL dH2O.
Well 7 (Vf = 20uL): Premix 20uL strawberry DNA (Ex #8) + 2.2uL
10X Loading dye
Due at the beginning of lab:
Lab Report for Ex #12 (making & running agarose gels)
Pre-Lab for Ex #13 (4h, P. 79, bacteria DNA extraction). Once you’ve
extracted bacteria DNA, follow protocols for NanoDrop (Ex 10) &
agarose gel electrophoresis (Ex9) to analyze your bacteria DNA. Load
gel as follows:
Hopefully you can extract the DNA, analyze it using the
NanoDrop, and make and run your gel in one class period!
During lab today, you must calculate the volume (uL) and mass
(ng) equivalencies of your DNA. For example, you need to load
250 ng DNA in Well 5. This is ng, not uL. So you must calculate
many uL of your DNA do you use if your stock DNA concentration
is 50ng/uL. This calculation can’t be done at home since you need
the NanoDrop data to calculate it. Please leave space in your prelab for the calculations.
Quiz #6
Reminder: Midterm is next Tuesday Oct 18, (2:30 – 4PM)
Midterm Tuesday Oct 18, (2:30 – 4PM)
Due at the beginning of lab:
Lab Report for Ex #13.
Review Midterm results; Exploring Science Careers Day
Ex #15 (8c, 8d): Bacteria Cell Culture & Transformation. Streak
pAmylase transformed E. coli DH5α competent cells onto plates.
Edison will refrigerate plates after 12-16hrs. Edison will pick
colonies for liquid cultures for next class.
-Prepare solutions for Ex #16-18.
Quiz #7
Ex #15 (8c, 8d): Finish Transformation Observations.
Practice picking a colony for liquid culture (inoculate LB broth
with Ampicillin).
(Dr. Mercer will review midterm material 2-2:30pm.)
Ex #14 (4e,f,g, and Making LB Agar Plates Containing Ampicillin)
4e: Making Liquid media (Part 1) & Agar plates (Part II)
4f: Pouring agar in to Plates
4g: Growing a Plate culture
Template Date – July 2013
Well 1 (Vf = 15uL): Load 15uL 1X DNA Loading dye (dilute 1:10 in dH2O).
Well 2 (Vf = 15uL): Load 15uL Lambda DNA cut with HindIII enzyme (=
Well 3 (Vf = 10uL): Load 10uL O’GeneRuler DNA ladder (ready to use)
Well 4 (Vf = 10uL): Load 10uL Bio-Rad 1kb Molecular Ruler (DNA ladder)
Well 5 (Vf = ?uL): Premix 250ng bacteria DNA + 10X Loading dye + dH2O
(you will have to calculate the volumes needed in your Protocol section of your
lab report). 250ng = x uL?
Well 6 (Vf = ?uL): Mix 500ng bacteria DNA + 10X Loading dye + dH2O in tube,
then load in gel. 500ng = x uL?
Well 7 (Vf = ?uL): Mix 1000ng bacteria DNA + 10X Loading dye + dH2O in
tube, then load in gel. 1000ng = x uL?
Well 8 (Vf = ?uL): Mix 2000ng bacteria DNA + 10X Loading dye + dH2O in
tube, then load in gel. 2000ng = x uL?
Today we will hear about some of the research being done here and locally,
by your fellow students, and watch some videos about careers in biotech.
Due at the beginning of lab:
Pre-Lab for Ex#14 (p71-79, 4e,f,g and Making LB Agar Plates
Containing Ampicillin)
Have read P.71-79 and Bb.
Have watched Bb “Making Microbiological Media” Video
Due at the beginning of lab:
You do not need to turn in a lab report for Ex#14. But you will use the
liquid media and agar plates you prepared to grow bacteria for Ex#15.
Pre-Lab for Ex #15 (8c, 8d, P. 159-165)
Have read P. 159-165.
Due at the beginning of lab:
Have read 8e (P166-7, FYI only). 8d and 8e are background. Do not
perform 8e.
Palm Beach State College
Course Syllabus – Classroom – Internet Component Class
Lab / Activity
The lab protocols in this course are taken directly from the
“Biotechnology Laboratory Manual” by Daugherty).
These protocols are also found on Blackboard (Bb).
Ex #16 (8f): Alkaline lysis Mini-prep of pAmylase plasmid DNA
(P. 167-170). “DIY” Plasmid Prep.
Ex #17: Qiagen mini-prep of plasmid DNA.
NanoDrop-Measure DNA quantity and quality from both the
Qiagen and 8f protocols.
Quiz #8
Ex#18: Restriction Digest of plasmid DNA. Use 8b protocol. P.
Prepare agarose gels during incubation step.
Ex #19: BioRad PV92 Kit: Cheek cell DNA isolation for PCR
using InstaGene Matrix. Setup and run PCR
Ex#19 (con’t): Make, load, and run agarose gels of PCR products
Quiz #10
Tuesday, November 22nd: Mandatory Field Trip: Students
must arrive on time, dressed professionally. No headphones, jeans,
or ripped T-shirts; as if on a job interview). No food or drink. Be
prepared to take notes on equipment and science presented. There
will be a quiz during (Quiz #11).
Thanksgiving. NO CLASS. Enjoy.
Due at the beginning of lab:
Lab Report for Ex#16 (8f)
Pre-lab for Ex#17 (Qiagen mini-prep).
Due at the beginning of lab:
Lab report for Ex#17
Pre-lab for Ex#18
Have read p155-158.
Ex# 18 continue: Run agarose gel from restriction digest of
transformed bacteria. Visualize gels. Discuss results.
Quiz #9
What is DUE:
*Your pre-lab is always due at the start of class.
*TLAB = Thinking Like A Biotechnician
Pre-lab for Ex#16 (8f), P167.
Ex #20: The Action of Different Enzymes on Apple Juice
Production (5b). P. 94-96 This is your last lab!
Lab wrap up. Review concepts. Students bring questions for
Final Exam.
Biotech Event (not during class, but important): Monday, Dec5
Poster Symposium (SC127, 1-3PM) The Biotech Program hosts its
Student Poster Symposium every semester. Biotech 2 students will
present their projects. Attendance at this event is highly
FINAL EXAM (last day of lab)
Due at the beginning of lab:
Lab Report for Ex#18
Pre-lab for Ex#19: BioRad PV92 PCR Kit (cheek cell)
Due at the beginning of lab:
Have read all Bio-Rad slides (Bb) on PCR and Hardy Weinberg.
Meet at 2pm at Scripps Florida, not at PBSC. Address: 630 Scripps Way,
Jupiter FL 33458 (Meet in lobby of Building B.)
Due at the beginning of lab:
Lab Report for Ex#19
Pre-Lab for Ex#20 (5b, p 94)
Due at the beginning of lab:
Lab Report for Ex#20
Department Contact Information
Name: Robert Van Der Velde, esq.
Telephone: 561-207-5416
Job Title: Associate Dean
Template Date – July 2013
Office Location: SC 160
Email: [email protected]
Fly UP