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LOYOLA COLLEGE (AUTONOMOUS), CHENNAI – 600 034

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LOYOLA COLLEGE (AUTONOMOUS), CHENNAI – 600 034
LOYOLA COLLEGE (AUTONOMOUS), CHENNAI – 600 034
M.Sc. DEGREE EXAMINATION - BIO TECHNOLOGY
SECOND SEMESTER – APRIL 2011
BT 2954 - MICROBIAL & BIOPROCESS TECHNOLOGY
Date : 07-04-2011
Time : 1:00 - 4:00
Dept. No.
Max. : 100 Marks
Section A
Answer all questions
I Choose the correct answer
1. ___________ grows between the pH ranges 5.8 to 8.
a) acidophiles
b) neutrophiles
c) basophiles
2. During fermentation the boiling appearance is due to the production of
a) oxygen
b) hydrogen
c) water
3. An example of an impeller is ____________.
a) rustom disc
b) simple brush
c) orifice
4. Destruction of spores requires _________ temperature for long periods.
a) 10°C
b) 40°C
c) 80°C
5. _____________ is used for wine production.
a) S.cerevisiae
b) S.uvarum
c) S.utilis
(20marks)
(5 x 1 = 5)
d) psychrophiles
d) carbondioxide
d) drive seal
d) 60°C
d) L.lactis
II Say whether the following statements are True or False; if false give reason
(5 x 1 = 5)
6. Organic compounds act as both electron donors & electron acceptors.
7. Hydroxylamine is used as base analogue.
8. Two-stage air lift reactor is used for temperature dependent formation of products.
9. Carboxy-methylcellulose is a common cationic exchange resins.
10. Kefir is produced from Candida sp.
.
III Complete the following
(5 x 1 = 5)
11._____________ is used universally for sterilization of media.
12._____________ is the cheapest source of carbohydrates.
13. _____________ is a role of computers in bioprocess.
14. _____________ membrane is used in absolute filters.
15. _____________ is a type of blue cheese.
IV Answer in one or two sentences, each in about 50 words
16. What are the different components of a fermentation process?
17. Explain the term scale-up process.
18. List five ideal features of a bioreactor.
19. Give an overview of effluent treatment protocols.
20. What is the role of economics and labor cost in fermentation technology?
(5 x 1 = 5)
Section B
V Answer the following, each answer within 500 words, draw diagrams wherever necessary
(5 x 8 = 40)
21. (a) Write notes on
(i) Sterilization of fermenter
(ii) Sterilization of liquid wastes
OR
(b) Briefly explain the range of fermentation process.
22. (a) Give a brief account on preservation of microorganisms.
OR
(b) Briefly explain strain improvement through mutagenesis.
23. (a) Give a detailed description on the body construction of a bioreactor.
OR
(b) Write notes on:
(i) Aeration and Agitation, Valves and Steam Traps
(ii) Photo bioreactor
24. (a) Explain liquid-liquid extraction.
OR
(b) Define cell disruption. Explain any four mechanical means of disruption.
25. (a) Explain the production and processing of sausages along with a schematic flowchart.
OR
(b) Explain the production of yogurt.
Section C
VI Answer any two of the following, each within 1200 words; draw diagrams wherever necessary
(2 x 20 = 40)
26. Describe the various environmental factors suitable for microbial growth.
27. Explain batch, continuous & fed-batch culture.
28. Explain in detail the different types of a bioreactor.
29. Give a detailed description on the production of gluconic acid and lactic acid.
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