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LOYOLA COLLEGE (AUTONOMOUS), CHENNAI – 600 034

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LOYOLA COLLEGE (AUTONOMOUS), CHENNAI – 600 034
LOYOLA COLLEGE (AUTONOMOUS), CHENNAI – 600 034
M.Sc. DEGREE EXAMINATION - BIO TECHNOLOGY
SECOND SEMESTER – APRIL 2008
KL 30
BT 2813 - GENETIC ENGINEERING
Date : 17/04/2008
Time : 1:00 - 4:00
Dept. No.
SECTION – A
Answer all the questions
Max. : 100 Marks
(20 marks)
I. Choose the best answer.
(5 X 1 = 5)
1. The concentration of each dNTPs used in PCR should be around ____ µM.
a) 50
b) 100
c) 150
d) 200
2. Which of the following is not true about the non-radioactive probes?
a) better stability
b) safety assured
c) costly equipment needed d) complicated detection
3. Which enzyme is a rare cutter?
a) Not I
b) Pst I
c) EcoR I
d) Hind III
4. Chromogenic agent used for bacterial transformation is
a) X-gal
b) IPTG
c) Glucose
d) Sucrose
5. ddNTPs (dideoxy NTPs) are used in
a) PCR
b) Sequencing c) Transcription
d) Translation
II. Say True or False; if false, state reasons.
(5 X 1 = 5)
6. Satellite DNA regions are significant candidates of choice in molecular probing.
7. The recombinant colonies are of blue colour in blue-white screening.
8. Shuttle vector has two origins of replication.
9. Transposons cannot be used for insertional mutagenesis.
10. Microarray is a technique exclusively used for DNA analysis.
III. Complete the following.
(5 X 1 = 5)
11. The formula for calculating Tm is ________________.
12. The standard primers used in PCR mutagenesis, for pGEM vector, are ____ and ____.
13. For bacterial transformation, heat shock is given at ________ temperature.
14. T-DNA stands for ____________.
15. Chemical sequencing was discovered by ________ and ___________.
IV. Answer the following in one or two sentences, each in about 50 words. (5 X 1 =5)
16. Dicer
17. WIPO
18. Subcloning
19. Spheroplast
20. In situ hybridization
SECTION – B
V. Answer any five questions, each in about 350 words only.
(5 X 8 = 40)
21. How is foot-printing technique advantageous over gel retardation assay?
22. What are the major topics of concern for ICGEB? Add a note on the Intellectual
Property Rights.
23. Explain the following: a) Insertional inactivation and b) Alpha complementation.
24. Enumerate the applications of RNA interference in various fields.
25. Differentiate between direct and indirect methods of gene transfer, and cite at least
two examples of each kind.
26. Explain insertional mutagenesis, with neat sketch.
27. Give an account on chain termination sequencing.
28. Distinguish between promoters and enhancers, with appropriate diagrams.
SECTION – C
VI. Answer the following, each in about 1500 words. (2 x 20 = 40)
29. a) Explain the principle and component parts of a PCR. Differentiate between real time and reverse
transcriptase PCR. How is nested-multiplexing used as a confirmatory diagnostic tool in medical field?
( 4 + 6 + 6 + 4)
.
(or)
b) ‘DNA microarray is a recent advancement in the field of molecular diagnostics, that combines
molecular biology and instrumentation science.’ – Justify this statement focusing on the construction,
principle of working and applications of DNA chips, with special reference to gene expression profiling.
( 6 + 6 + 8)
30. a) Explain and show diagrammatically the production of recombinant human insulin.
Add a note on pharma products produced by Genetic Engineering and their uses.
(or)
b) What are the methods employed for gene transfer in animal cells and explain them.
Give short note on patenting of GMOs.
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