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LOYOLA COLLEGE (AUTONOMOUS), CHENNAI – 600 034 Section-A

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LOYOLA COLLEGE (AUTONOMOUS), CHENNAI – 600 034 Section-A
LOYOLA COLLEGE (AUTONOMOUS), CHENNAI – 600 034
M.Sc. DEGREE EXAMINATION – BIO MED. INSTRU. SCIENCE
FIRST SEMESTER – NOVEMBER 2010
BI 1808 - TOOLS & TECHNIQUES IN CELL BIOLOGY
Date : 30-10-10
Time : 1:00 - 4:00
Dept. No.
Max. : 100 Marks
Section-A
Answer all the questions
(10x2=20)
1. Differentiate freeze-etching from fractioning of frozen tissue.
2. Define X-ray diffraction.
3. What are proto –oncogenes?
4. What are microtubules and associated proteins?
5. Mention any four properties of stains.
6. Define cell lines with examples.
7. Define in situ hybridization.
8. What is biothesiometry?
9. Give the principle of autoanalyzer.
10. Explain the nucleolar cycle.
Section-B
Answer any four questions
(4x10=40)
11. DNA fingerprinting is a significant tool in genotype analysis-Justify.
12. Describe Poly Acrylamide Gel Electrophoresis and its applications in cellular studies.
13. Elaborate the sequence of events and process leading to cell reproduction.
14. Explain the special features of ultra centrifugation and its applications.
15. Explain the ultra structure and functions of mitochondria.
16. Write notes on a) Spherical aberration b) Scanning Electron Microscope.
Section-C
Answer any two questions
(2x20=40)
17. Give an account on polymerase chain reaction and its applications in biomedical research.
18. Explain the different media used in tissue culture. What are the advantages of each media?
19. Explain the nucleic acid hybridization technology in context of diseases diagnosis.
20. Give a detailed account on High Performance Liquid Chromatography.
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